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Citations for Roche :
1 - 50 of 754 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... from tissue lysates and assayed for Gli1 gene expression with SYBR-Green (Roche) on a LightCycler 480 (Roche).
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bioRxiv - Molecular Biology 2020Quote: Whole tissue or cell lysates were produced in RIPA buffer supplemented with PhosSTOP (Roche Diagnostics Corporation ...
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bioRxiv - Microbiology 2022Quote: ... Porcine trypsin and the Protease Inhibitors Set (Roche) were purchased from Sigma ...
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bioRxiv - Pathology 2022Quote: Tissue and cell lysates were produced in RIPA buffer supplemented with PhosSTOP (Roche Diagnostics, Indianapolis, IN) and protease inhibitor cocktail (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2023Quote: Tissue homogenates or cell lysates were prepared in RIPA lysis buffer supplemented with protease inhibitors (Roche) and subjected to Western blotting as previously described30 ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
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bioRxiv - Genomics 2020Quote: ... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
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bioRxiv - Neuroscience 2021Quote: Proteins from brain tissue lysates or primary cortical neurons were extracted using RIPA buffer with protease and phosphatase inhibitor cocktails (Roche). Total lysates were obtained by 30 sec ...
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bioRxiv - Cancer Biology 2021Quote: Lysates were prepared from tumor tissue or cultured cells using RIPA buffer supplemented with fresh protease and phosphatase inhibitors (Roche). Protein quantification was performed using the BCA Protein Assay (G Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: Lysates were incubated with GFP (Roche), Muskelin (Boster) ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis of cytokine related porcine gene expression was performed using the KAPA SYBR-FAST qPCR Kit (KAPA Biosystems), using the primers shown in Table 1 ...
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bioRxiv - Biophysics 2019Quote: ... Lysates were incubated with Ni-NTA beads (Roche). After washing with the buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysate was bound to IgG-Sepharose beads (Roche) and washed by centrifugation with 100 mM NaCl ...
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bioRxiv - Genetics 2020Quote: ... The lysate pretreated with RNase (RNase+ [10μg/ml, Roche]) was used as negative control (RNase+ control) ...
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bioRxiv - Cell Biology 2022Quote: ... the lysates were incubated with G-agarose beads (Roche) and rotated for 4 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Bacteria lysates were incubated with Ni+ resin (Roche cOmplete) for 2h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... All tissue samples were processed on the Ventana BenchMark ULTRA system (Roche Tissue Diagnostics). Information on antibodies used are in Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was incubated with cOmplete Ni-NTA resin (Roche) for 2h at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Lysates were treated with 600 U of RNase S7 (Roche) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was affinity purified by Ni-NTA beads (Roche) and the eluted protein was further purified by size-exclusion chromatography on HiLoad Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Roche Tissues Diagnostics) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat #760-159) ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissue was dissociated using Liberase (Roche) and cells were harvested by FACS using a BD FACSAria II (BD Biosciences) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... High Pure RNA Tissue Kit (Roche) or NZY Total RNA isolation kit (NZYtech) ...
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bioRxiv - Genetics 2020Quote: ... 400 µL Tissue Lysis Buffer (Roche) and 50 µL Proteinase K (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were incubated with Liberase (Roche) (0.3 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... DAPI (Roche Tissue Diagnostics, ref. 05268826001) was used as a nuclear counterstain ...
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bioRxiv - Cancer Biology 2024Quote: ... (Roche tissue Diagnostics, ref.760-1029) and Negative Control Mouse Monoclonal Antibody (MOPC-211) ...
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bioRxiv - Cancer Biology 2024Quote: ... (Roche tissue Diagnostics, ref. 760-2014) and used on each multiplex staining run.
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bioRxiv - Developmental Biology 2021Quote: ... Whole brain lysate was precleared with Protein G agarose (Roche, 11243233001) and the supernatant was immunoprecipitated with CB1R antibody overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The lysate was pre-cleared with protein G-agarose beads (Roche) and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell lysate was first purified using Ni-NTA beads (Roche) and then subjected to size-exclusion chromatography on HiLoad Superdex 200 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Lysate was resuspended in 2x protease inhibitor solution (cOmplete mini, Roche) and centrifuged for 10 min at 14,000 rpm and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were pre-cleared with protein A-agarose beads (Roche) for 3 h and then incubated with anti-β1-integrin antibodies (MAB2000 from Millipore ...
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bioRxiv - Zoology 2021Quote: ... was determined from the cell lysate using an ELISA kit (Roche) in accordance with the company instructions ...
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bioRxiv - Cancer Biology 2021Quote: RLT Plus lysates with Complete Protease Inhibitor (Roche, catalog no. 11836170001) homogenized as described in the ‘RNA sequencing’ section were passed through the RNA binding column from the AllPrep DNA/RNA Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate was incubated for 30 min on ice with DNAseI (Roche) and ultracentrifuged at 120 000 g / 4°C / 40 min ...
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bioRxiv - Microbiology 2023Quote: ... phage lysates were treated with RNase A (Roche; 1 μg/mL), DNase I (NEB ...
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bioRxiv - Immunology 2023Quote: ... except cleared lysates were incubated with anti-HA matrix (11815016001, Roche) (pre-blocked with 1% BSA-PBS for 1h ...
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bioRxiv - Cancer Biology 2019Quote: ... First the tissue was mechanically dissociated using a tissue grinder while submerged in 1 ml of TriPure (Roche). 200 µl of chloroform (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to cOmplete His-Tag purification resin (Roche), pre-equilibrated in lysis buffer and incubated at 4 °C for 6 h on a tube roller ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was incubated with RNAse T1 (20 U/μL, Roche), RNase A (20 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Cleared lysates were subjected to immunoprecipitation using anti-GFP antibody (Roche 11814460001) conjugated to protein G magnetic beads (Thermofisher ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were treated with RNase-free DNase I (10 U, Roche) for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were prepared in lysis buffer supplemented with protease inhibitors (Roche) as described previously ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were prepared in RTK lysis buffer with protease (11836153001, Roche) and phosphatase (04906837001 ...
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bioRxiv - Immunology 2022Quote: Cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Roche) and PMSF ...