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Citations for Roche :
401 - 450 of 4733 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 1μg of total RNA was subjected to reverse transcription with random primers using Takera’s TranscriptorFirst Strand cDNA Synthesis Kit and cDNA was subjected to real-time PCR analyses using LightCycler 480 SYBR Green I Master Mix (Roche) in a LightCycler 480 Instrument(BD ...
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bioRxiv - Immunology 2020Quote: The reaction mix per sample for quantitative real-rime PCR (qPCR) contained: 5 µl of the LightCycler 480 SYBR Green I Master Mix (04707516001, Roche), 2.5 µl cDNA and 2.5 µl primers in a total reaction volume of 10 ul ...
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bioRxiv - Zoology 2020Quote: ... A 25 μl PCR master mix was prepared in a UV-sterilized hood and consisted of 3.125 μl Buffer (Kapa Biosystems), 1.25 μl dNTP (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... The final PCR reactions to produce Illumina-compatible sequencing libraries were prepared from the entire amplified cDNA using KAPA HiFi PCR Master mix (Roche) at 98 C for 2 min ...
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bioRxiv - Plant Biology 2021Quote: ... Expression levels of germination-associated genes were measured by qRT-PCR analysis using LightCycler 480 SYBR Green I Master mix (Roche) in a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Physiology 2021Quote: ... gDNA regions were amplified via PCR using 1/150th gDNA and 300nM forward and reverse primers in a 50 μL FastStart PCR Master Mix reaction (Roche). PCR products were purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... was performed with NZY Speedy qPCR Green Master mix (NZY tech) and in a LightCycler 480 Real-Time PCR System (Roche). Primer sequences are detailed in the Supplementary Table S8 ...
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bioRxiv - Plant Biology 2021Quote: ... Two µL of diluted cDNA was used as a template for the qRT-PCR with Fast SYBR Green Master Mix (Roche) on a Light-Cycler 96 instrument (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... the cDNA samples were amplified using LightCycler® 480 SYBR® Green 2x PCR Master Mix I (Roche, Cat# 04887352001) and 0.3 or 0.6 μM of forward and reverse primer respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-Time qRT-PCR was performed using the Real-Time 2xHS-PCR Master Mix Sybr B (A&A Biotechnology) on a LightCycler 480 II qPCR System (Roche) with custom-designed primers for Gli1 (Fwd ...
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bioRxiv - Microbiology 2022Quote: ... the V4 region of the 16S rRNA gene was amplified with barcode primers containing the index sequences using a KAPA HiFi HotStart Real-time PCR Master Mix (Roche). We monitored PCR product amplification and concentration on a Bio-Rad CFT Connect Real-Time PCR system ...
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bioRxiv - Microbiology 2022Quote: ... 33.75 µL cells were mixed with a PCR master mix containing 37.5 µL KAPA HiFi HotStart ReadyMix (Roche, CN KK2602), 2.25 µL second strand synthesis primer SMRT_dT (10 µM ...
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bioRxiv - Plant Biology 2022Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using qPCR primer pairs listed in Table S2 and LightCycler480 SYBR Green I Master Mix (Roche) in a LightCycler480 instrument ...
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bioRxiv - Neuroscience 2023Quote: ... The vectors containing pegRNA and PE3/PE3b guides were used as template for PCR using KAPA HiFi HotStart DNA Polymerase with 2x Master Mix (Roche) to amplify parts containing the U6 promoter and either the pegRNA or PE3/PE3b guide using primers with BsaI recognition sites ...
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bioRxiv - Developmental Biology 2023Quote: ... The real-time PCR was performed with Roche LightCycler 480 SYBR GREEN I Master mix on a LightCycler 480 system (Roche). Relative gene expression abundance between samples was calculated with the CT method using Gapdh as an internal control ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Cell Biology 2022Quote: ... Two different HRMA-compatible platforms were used (Applied Biosystems ViiA 7 Real-Time PCR System, using the MeltDoctor HRM Master Mix, and Roche LightCycler 480 System ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the cDNA samples were amplified using LightCycler® 480 SYBR® Green 2x PCR Master Mix I (Roche, Cat# 04887352001) and 0.6 μM of forward and reverse primers with a LightCycler® 480 (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... The qPCR reactions were performed in a 20 μL reaction mixture using SYBR Green PCR Master Mix (Roche, Madison, WI, USA). The primers were as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... All PCR reactions were performed using SYBR-containing master mix from the KAPA Real-Time Library Amplification Kit (Kapa Biosystems #KK2702) and terminated in the mid-exponential phase to limit over-amplification ...
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bioRxiv - Genomics 2020Quote: ... Junction PCR was performed in quintuplicate for each replicate with 20 uL cDNA and 10 uM input Forward and Reverse Junction Primers (Table S16) using KAPA HiFi 2x PCR Master Mix (KAPA Biosystems) with the following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μg of gDNA from each sample was amplified in 20 individual PCR reactions using Kapa HiFi Master Mix (Kapa Biosystems) using TKOv3 library primers (forward ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We performed qRT-PCR with SYBR I Green Master Mix and a Roche LightCycler 480 (Roche Applied Science, Indianapolis, IN, USA) following the manufacturer’s protocol with triple technical replicates of 10 μl reactions (n = 20 for each parental context ...
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bioRxiv - Molecular Biology 2023Quote: ... The same proportion of cDNA products of each sample was used as template for the quantitative RT-PCR reaction with Light Cycler 480 SYBR Green I Master Mix (Roche, 04707516001). The Ct value was used to represent the absolute amount of EU-RNAs in each sample ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in technical duplicate or triplicate using SYBR FAST qPCR Master Mix (Kapa Biosystems, Cape Town, South Africa) on a LightCycler 480 (Roche Applied Science ...
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bioRxiv - Neuroscience 2024Quote: ... titer was determined at a concentration of 1.1013 gcp/ml by quantitative real-time PCR using the Light Cycler 480 SYBR green master mix (Roche, cat # 04887352001) with primers specific for the AAV2 ITRs (fwd 50-GGAACCCCTAGTGATG GAGTT-3’ ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR was performed by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system ...
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bioRxiv - Microbiology 2021Quote: ... 18 µL of qPCR master mix containing 10 µL of SYBR Green I Master (04707516001, Roche), 7.6 µL of nuclease-free PCR grade water ...
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bioRxiv - Immunology 2021Quote: ... using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Bioengineering 2022Quote: ... cDNA was prepared together with SYBR Green Master mix (Roche) using Bravo liquid handling platform (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was done using SYBR Green I Master Mix (Roche) on a Roche Light Cycler 480 with forward and reverse oligonucleotide primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Molecular Biology 2020Quote: ... and qPCR (KAPA SYBR FAST qPCR Master Mix, Kapa Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... using the Fast Start Universal SYBR Green Master Mix (Roche). ABI 7500 platform (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The LightCycler SYBR Green I Master reaction mix (Roche®) was used with the following volumes for each sample analyzed ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA and LightCycler 480 SYBR Green I Master mix (Roche) in a final volume of 10 μl ...
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bioRxiv - Microbiology 2021Quote: ... 12.5 µL of HiFi Hot Start Master Mix (Kapa Biosystems) and 9 µL of nuclease-free water ...
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bioRxiv - Plant Biology 2021Quote: ... Reactions were performed using SYBR Green I Master Mix (Roche) and the manufacturer’s recommended protocol (pre-incubation at 95°C for 5m ...
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bioRxiv - Molecular Biology 2021Quote: ... and Roche SybrGreen Master Mix reagent (SybrGreen, Roche, Grenzacherstrasse, Switzerland). For each gene ...
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bioRxiv - Developmental Biology 2020Quote: ... 10 µl SYBR Green I Master Mix (Roche Applied Science) and 200 nM of each gene-specific primer ...
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bioRxiv - Cell Biology 2022Quote: ... using the LightCycler 480 SYBR Green I Master mix (Roche). LMNA gene expression was probed using the primer sequences in Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... with Universal SYBR Green Master Mix (4913914001; Roche, Shanghai, China). β-actin was used as an internal control ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed using FastStart SYBR green master mix (Roche) in a Viia 7 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed by using SYBR Green master mix (Roche) in Light-cycler 480 (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... with the polymerase ready mix (SYBR Green I Master, Roche). Relative quantification was performed using the comparative CT method (Schmittgen and Livak ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was prepared with SYBR Green Master Mix (Roche, #04887352001) using the Bravo instrument (Agilent ...