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Citations for Roche :
4651 - 4700 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 2 mM MgCl2, 0.3 % Triton X-100, 0.25 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by sonication using Diagenode Bioruptor for 10 min (30 sec ON ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100, and 1 mM EDTA) containing a phosphatase inhibitor cocktail (Nacalai Tesque, Kyoto, Japan) and protease inhibitor cocktail (Roche, Basel, Switzerland). After centrifugation at 20,380×g for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 5 mM imidazole, 0.4% Triton X-100, supplemented with Roche cOmplete Protease Inhibitor Cocktail) and lysed by sonication ...
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bioRxiv - Biochemistry 2021Quote: ... washed twice with PBS and subsequently resuspended in assay buffer (20 mM HEPES pH 7.5, 150 mM NaCl, and 0.1% Triton X-100 supplemented with Roche cOmplete Protease Inhibitor Cocktail). The cells were lysed by sonication and cleared by centrifugation for 5 min at 14’000 rpm and 4°C ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into HeLa cells growing on 100 mm dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols and processed for TEM at 18 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: Frozen yeast lysate powder was resuspended in ice-cold lysis buffer (50M Tris-HCl, pH 7.4, 150mM NaCl, 0.05% Triton X-100, 1x Roche cOmplete Proteinase inhibitor cocktail) to a concentration of 6µg/µl and rotated for 10 min at 4°C before centrifugation at 3000 x g for 10min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... 140 mM NaCl, 1mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.1% Na-Deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 200 µL Roche cOmplete protease inhibitors). 0.5 mm diameter glass beads were added to 1 mm below the meniscus ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl2, 1% Triton X-100, 10% Glycerol, 1 mM DTT, Roche complete protease inhibitor) with 0.5 mg/mL Lysozyme and placed on a roller for 45 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were collected and lysed in lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Diagnostics, Mannheim, Germany]) at 4°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... or 48 h after seeding in a Triton X-100-based buffer (150 mM NaCl, 20 mM Tris-HCl pH 7.5, 5 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). A linear sucrose density gradient was prepared in 13x51 mm centrifuge tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1mM EGTA, 1% Triton X-100, 0.4% SDS, 1% NP-40, 1× Roche protease inhibitor cocktail), one time with wash buffer B (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... tubes and homogenized for 1.5 minutes in 50μL of TBS containing 1% Triton X-100 (Table 1) and 1X protease and 1X phosphatase inhibitor cocktails (Roche, 04693124001 and 04906837001). The homogenates were then probe-sonicated with a Branson Sonifier® SFX150 (Emerson ...
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bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 1x cOmplete protease inhibitor cocktail [Roche-11697498001], 1 mM PMSF), incubated on ice for 10 minutes and centrifuged for 10 minutes at 21100 x g at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... Cells were resuspended in ½ volume ice-cold NE1 buffer (20 mM HEPES-KOH pH 7.9, 10 mM KCl, 0.5mM spermidine, 0.1% Triton-X-100, 20% glycerol with Roche Complete Protease Inhibitor EDTA-free) and lysed on ice for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in immunoprecipitation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% TritonX-100 supplemented with Roche protease inhibitor cocktail). Lysates were centrifuged (5,000x g ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 0.1 mM EGTA, 100 mM NaCl, 1% Nonidet P-40, 10 mM Imidazole, supplemented with Roche protease inhibitor cocktail). Cell lysates were clarified by centrifugation at 10,000× g for 5 min at 4 °C and then incubated at 4 °C under constant rotation with His-ΔFIP1c-RCP559-649 fusion protein ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA, 1% SDS, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride, and 1× Roche Protease Inhibitor Cocktail) and sonicated to reduce the average DNA fragment size to ∼500 bp ...
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bioRxiv - Microbiology 2023Quote: ... Purified PARP10 was diluted to 20 µM using MARylation buffer (SEC buffer supplemented with 0.02% Triton X-100 and 200 µM β-NAD+ (Roche, NAD100-RO)) ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Neuroscience 2023Quote: Lysates from cultured neurons were collected in NP40 lysis buffer (150 mM NaCl, 100 mM Tris-HCl, 1% NP40 in PBS) containing 1X protease inhibitors (cOmplete ULTRA tablets, Roche, cat# 05892970001). Samples were run on mini-protean TGX gels (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Developmental Biology 2024Quote: ... the extirpated tongues from non- transgenic and Lgr5-EGFP-IRES-CreERT2;R26-mTmG reporter mice were injected with approximately 100-200 μl 1% Dispase II (neutral protease; Roche, Indianapolis, IN) dissolved in Balanced Salt Solution (BSS ...
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bioRxiv - Microbiology 2024Quote: ... Samples were lysed in 1% Triton X-100 (20 mM Tris [pH 7], 150 mM NaCl, 5 mM MgCl2) containing protease inhibitor (Roche, Cat# 11697498001) with end-over-end rotation for 20 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 5% Sarkosyl) and samples were treated with proteinase K (50μg/mL) and Ribonuclease A (Roche) for 30 minutes at 37°C to remove contaminating proteins and RNA ...
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bioRxiv - Microbiology 2020Quote: Fresh cultures were inoculated from glycerol stocks into 400μl 7H9 with kanamycin (Roche, 20ug/ml) and grown until saturated ...
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bioRxiv - Microbiology 2020Quote: ... Exponential phase cultures were inoculated 1:40 into 7H9 supplemented with kanamycin (Roche, 20ug/ml) and ATc (Sigma 100ng/ml ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed in 2X PIC (Roche mini-tabs, 1 tab in 5 ml = 2X) and stored at -80°C until needed.
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bioRxiv - Neuroscience 2021Quote: ... 0.3mg or 1mg/ml/kg midazolam (Hypnovel, Roche, diluted with 0/9% w/v saline) fifteen minutes prior to a standard punishment session ...
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bioRxiv - Molecular Biology 2021Quote: ... The tissue was minced and transferred to Enzyme cocktail [0.25 mg/mL Liberase TH (Roche), 20 U/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with labeled probe and 250 µg/mL salmon sperm DNA (Roche) at 65°C overnight in Perfecthyb Plus Hybridization Buffer (Millipore) ...
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bioRxiv - Immunology 2019Quote: ... The lungs were removed and infiltrated with 2 mg/ml collagenase D (Roche, Indianapolis, Indiana) and 0.2 mg/ml DNase I (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM DTT) containing Complete™ Protease Inhibitor Cocktail (one tablet per 50 mL) (Roche) and disrupted by sonication (Branson Digital Sonifier ...
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bioRxiv - Cell Biology 2019Quote: ... Two ml of ice-cold lysis buffer supplemented with protease inhibitors (Roche Applied Science, 11836145001), phosphatase inhibitors (Calbiochem ...
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bioRxiv - Genetics 2021Quote: ... Pellets were thawed on ice and 750 ml of FA buffer containing protease inhibitors (Roche Cat#11697498001 Complete Protease Inhibitor Cocktail Tablet ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 30 μl of 20 mg/ml proteinase K (Roche 03 115 828 001). The samples were incubated overnight at 55 °C ...
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bioRxiv - Immunology 2021Quote: ... Then fragments were digested in RPMI 1640 medium containing Liberase™ (Roche, 0.05 U/mL) plus DNase I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with one EDTA-free protease inhibitor mini tablet / 5 ml of buffer (Roche #4693159001). Lysates were incubated on ice for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... 1 mg/mL DNAse and one complete mini EDTA-free protease inhibitor cocktail tablet (Roche), by passing the sample three times through a pressurised cell disruptor (M110-L ...
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bioRxiv - Bioengineering 2020Quote: ... antigens were retrieved using 1 mg/ml pronase/PBS (11459643001; Roche life science, Indiana, USA) and 10 mg/ml hyaluronidase/PBS (H2126 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was reconstituted in 10 mL PBS containing protease and phosphatase inhibitors (Roche) and centrifuged again at 1100 RPM for 4 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The heads or brains were then digested with 10ug/mL Proteinase K (Roche, RPROTK-RO) for 10-20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then digested by addition of 2 μL 20 mg/mL Proteinase K (Roche) and incubation for 2 h at 55° C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was reconstituted in 10 mL PBS containing protease and phosphatase inhibitors (Roche) and centrifuged again at 1100 RPM for 4 minutes ...