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Citations for Roche :
4601 - 4650 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... minced with razor blade and digested enzymatically with 3 mg/mL collagenase/dispase (Roche) at 37 °C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... the stripped endothelium–Descemet’s layer was incubated with 2 mg/ml collagenase A (Roche) solution in human endothelial serum free media (SFM ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05 mg/mL of DNaseI (Cat. No. 10104159001, Roche/Sigma-Aldrich, 100mg from bovine), and 1% BSA (Sigma-Aldrich Pty Ltd ...
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bioRxiv - Microbiology 2023Quote: ... 5% Glycerol) complemented with lysozyme (0.25 mg/ml; Merk) and cOmplete Protease Inhibitor (Roche). The samples were sonicated and centrifuged at 15,000g for 45 min at 4°C to pellet cell debris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 20 µg mL-1 luciferase reagent (ATP Bioluminescence Assay Kit CLS-II, Roche). The reaction was initiated with 200 µM NADH ...
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bioRxiv - Cell Biology 2023Quote: ... Minced adipose tissue was incubated with 0.28 U/ml of liberase TM (Roche #05401119001) in DPBS with calcium and magnesium (Gibco #14040091 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 ml per 2.5×107 cells) supplemented by EDTA-free complete protease inhibitor (Roche). Lysate was prepared by three cycles of freeze-thawing ...
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bioRxiv - Neuroscience 2024Quote: ... and sections were permeabilized using proteinase K (10 µg/mL in PBS-DEPC, Roche) for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and protease inhibitor (1 pill per 10 ml; cOmplete Mini EDTA-free, Roche Diagnostics). The lysates were sonicated and then centrifuged at 14,000 x g for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was treated with 2 mg/mL of pronase from Streptomyces griseus (Roche) in 50 mM Tris-HCl pH 7.0 for 1.5 h at 60°C ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mg/mL DNaseI (Gold Biochem) and a single “Complete” protease inhibitor tablet (Roche). Cells were lysed by sonication and the lysate clarified by centrifugation and applied to glutathione agarose (1 mL bed volume ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumors were digested by incubation in liberase TM (0.05 mg/ml; Roche, #05401119001)+DNAse (0.2 mg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Lumenal Act PBMCs were activated with Phytohemagglutinin-M 10 µg ml−1 (Roche, Indiana), with cell concentration maintained at 2,000 cells μl−1.
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bioRxiv - Synthetic Biology 2023Quote: ... 1 ml per 2.5×107 cells) supplemented by EDTA-free complete protease inhibitor (Roche). Lysate was prepared by three cycles of freeze-thawing ...
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bioRxiv - Microbiology 2023Quote: ... filtered through 0.45 μm filter and treated with 10 U/ml DNase I (Roche) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 0.25 units mL-1 of benzonase and protease inhibitor mix (cOmplete EDTA free, Roche). Cells were lysed using the EmulsiFlex-C5 (15,000 psi ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos were treated with 10 μg/mL proteinase K (Roche, catalog no. 3115887001) in PBST for a duration of 10 to 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The tissues were then digested with 50 μg/mL DNase I (Roche, Cat#: 10104159001) and 300[U/mL collagenase VIII (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.8mM CaCl2) supplemented with 250uL of 5mg/mL Liberase (Roche, 05 401 127 001) and incubated for 2h30-3h at room temperature (RT ...
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bioRxiv - Developmental Biology 2024Quote: ... then 500 μL of a dissociation solution containing 0.1 mg/mL Liberase TM (Roche) and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... were purchased from Lonza and cultured in coated flasks with 50 μg/ml rat tail collagen I (Roche), with Vasculife (LL-0003 ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed in L-15 medium with 40 μg/ml DNaseI (Roche) and transfected with 2 μg plasmid DNA using the 4D- Nucleofector (Lonza) ...
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bioRxiv - Physiology 2024Quote: ... The final pellets were suspended in 200 μL of 0.3 mg/ml amyloglucosidase (Roche) in 0.2 M sodium acetate (pH 4.8) ...
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bioRxiv - Immunology 2024Quote: ... the cells (108/ml) were pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM ...
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bioRxiv - Biochemistry 2024Quote: We diced lung tissue samples and digested them with 0.25 mg/ml Liberase (Roche) and 1 mg/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently adjusted 105 cells/ml cell densities using a CASY cell counter (Roche). Three µl of fungal cell suspensions were applied topically to the center of the biopsies ...
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bioRxiv - Neuroscience 2024Quote: ... containing 0.5% BSA and 3 mg/mL DNAse I grade II (Roche, Cat# 104159). Once resuspended ...
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bioRxiv - Biophysics 2024Quote: ... 70 μg/ml lysozyme and cOmplete mini EDTA-free protease inhibitor cocktail (Roche, 11836170001)] per liter of cultured E ...
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bioRxiv - Immunology 2020Quote: ... Lyzed cells were centrifuged at 20,000 x g for 30 min at 4°C and the supernatant was mixed with protease inhibitor (cOmplete ULTRA Tablets, Roche, Sigma-Aldrich, Cat: 05892953001). Protein concentration was determined using the Qubit Protein Assay Kit and Fluorometer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... expanded leaves of 7-week old hybrid aspen plants were sampled with a biopsy punch (4 mm diameter) and loaded into wells of 96-well plate (Roche LightCycler® 480 System) containing 100 μL of water (one sample per well) ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded in conditioned medium in triplicate in 6 cm plates and allowed to attach for 4 h before treatment with mitomycin C (Roche 10 107 409 001) or olaparib (Selleckchem AZD2281 ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes were hybridized at 56°C overnight and subsequently detected by anti-DIG at 4°C overnight (1:1000; Roche 11-207-733-910) with secondary tyramide signal amplification for 2h at room temperature in the dark (AKOYA Biosciences ...
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... Samples were pre-incubated for 2 h in blocking solution (25% deactivated goat serum in PBT) and incubated overnight at 4°C in blocking solution with anti-DIG antibody conjugated to alkaline phosphatase (Roche Diagnostics, Germany; 1:2000). After several washes in PBT ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole cell lysates were extracted from trans transfected 293T cells or infected LNCaP cells or PC-3 cells by IP lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). An aliquot of the cell lysate was kept as input for western blot analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Cell Biology 2022Quote: ... cells were first thawed on ice and resuspended in 1 mL FA buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate, with Roche cOmplete protease inhibitors (Sigma)) ...
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bioRxiv - Genetics 2019Quote: ... of each library was used for bar-coding amplification using dual-barcoded single-stranded library adapters44 as primers in the following 100 μl volume reaction: 10 μl 10x PCR Buffer + MgCl2 (Roche, Basel, Switzerland) 0.4 μM of each primer ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were harvested with 500 µl RIPA-buffer (Tris-HCl, NaCl, Triton X-100, sodium deoxycholate, SDS, EDTA, pH 7.4) with Complete protease inhibitor (Roche Diagnostics, Basel, Switzerland) and protein concentration was measured using a Bradford assay (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... vortexed in 200 µL ice-cold extraction buffer (20 mM Tris pH 7.5, 300 mM NaCl, 5 mM MgCl2, 0.1% Triton X-100, 1x complete Proteinase-Inhibitor Cocktail from Roche, 50 µM MG-132) and incubated for ten minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... the resulting cell pellets were re-suspended in 500 uL of lysis buffer (1X PBS, 0,5% Triton X-100, cOmplete EDTA-free protease inhibitor cocktail, Roche Diagnostics, Basel, Switzerland) and incubated for 10 min on ice ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were harvested by centrifugation, resuspended in lysis buffer (20 mM Tris, 100 mM NaCl, 20 mM imidazol, protease inhibitor (Roche, Mannheim, Germany)) and lysed by 3-4 passages through Emulsiflex B15 (Avestin ...
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bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in French Press buffer (40 mM HEPES, 100 mM KCl, 20 mM imidazole, 2 mM PMSF, EDTA-free cOmplete protease inhibitor [Roche], pH 7.5) for 1 hour followed by homogenization using tight douncer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in lysis buffer (25 mM HEPES pH 7.5, 400 mM NaCl, 10 mM 2-mercaptoethanol, 0.1% Triton X-100 supplemented with lysozyme and Roche cOmplete protease inhibitor tablet), lysed by sonication (50% duty cycle ...
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bioRxiv - Immunology 2019Quote: ... and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS, 1% Triton X-100, 2mM EDTA, 50mM Hepes-KOH [pH 7.5], 0.1% sodium deoxycholate, Roche 1X Complete Protease Inhibitor). Nuclei were lysed for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...