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Citations for Roche :
4451 - 4500 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the beads were collected on a magnetic stand for 2 min and washed four times with lysis buffer and four times with wash buffer (50mM Tris pH 7.6, 300mM NaCl, 1% Triton X-100, + complete protease inhibitor/ Roche Cat# 11836153001). The beads were resuspended in 1x Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in 100 μl cold RIPA buffer (Pierce, 8990) supplemented with cOmplete mini EDTA-free protease inhibitor cocktail (Roche, 11836170001). Protein abundance was measured by Qubit ...
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bioRxiv - Microbiology 2022Quote: ... Nuclei from 600,000 cells were isolated by hypotonic lysis in 1 mL buffer NE1 (20 mM HEPES-KOH pH 7.9; 10 mM KCl; 0.5 mM spermidine; 0.1% Triton X-100; 20% Glycerol; Roche EDTA-free protease inhibitor) for 10 minutes on ice followed by centrifugation at 1300 xg for 4 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA cassettes were amplified using 22 cycles of PCR amplification from approximately 300 µg genomic DNA (10 µg DNA/100 µL reaction) using 2X KAPA HiFi HotStart ReadyMix (Roche KK2602), a combination of 8 staggered P5 primers ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were then isolated by incubating with NE1 buffer (10 mM HEPES pH7.9, 10 mM KCl, 0.1% Triton X-100, 20% glycerol, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 10 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM NaCl, 2 mM MgCl2, 10 mM NaF, 1 mM PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 45 min on ice and centrifuged at 20000g for 10 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: Cell harvesting and disruption were performed with the multi shot cell disruptor at 20,000 psi using buffer A (100 mM Tris-HCl, pH 8.0) with cOmplete™ Protease inhibitor (Roche, Basel, Switzerland) for cell resuspension and disruption ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad laboratories GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Triton X-100, 0.2% Nonidet P-40, 0.6 mM PMSF, and 20 µM MG132 in a Roche protease inhibitor cocktail) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in lysis buffer (100 mM HEPES pH 7.5, 200 mM NaCl, 10% glycerol) supplemented with EDTA-free Protease inhibitor cocktail (Roche, Cat#05056489001), 1 mM phenylmethylsulfonylfluoride (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... extract was prepared using Co-IP buffer (50 mM Tris/Cl (pH=7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001), 0.5 mM PMSF) ...
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bioRxiv - Physiology 2023Quote: ... of flash−frozen liver tissue were lysed and homogenized with sucrose lysis buffer (50 mM HEPES, 150nM NaCl, 0.25M Sucrose, 0.4nM AEBSF, 1:100 Igepal) with complete protease inhibitor (Roche Diagnostics, Mannheim, Germany). Lysate samples were centrifuged at 15,000 g for 10 min ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 100 mg of tobacco leaf tissue using the TriPure reagent (Roche Sigma-Aldrich, St. Louis, MO), according to the manufacturer’s instructions ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Cells were scrapped into 100 µl of RIPA lysis buffer (Astral Scientific 786-490) supplemented with protease inhibitor cocktail (Roche 11697498001) and transferred to microfuge tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared by incubation in Onyx lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Molecular Biochemicals]) at 4 °C for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... a 6mm section of the infarcted brains was dissected and homogenised to 300mg wet weight of tissue per 1ml of Lysis Buffer (TBS +1% Triton X-100; Roche Australia).
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... The discs were dissected in pre-cooled PBS and pipetted in PBT (PBS, 0.1 % Triton X-100) containing 1x proteases inhibitors (cOmplete™ Protease Inhibitor Cocktail tablets, Roche, 11873580001). The disks were incubated in PBT with 1x protease inhibitors and 0.4 % IGEPAL CO-630 (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... Seeds were selected on germination medium containing 15 µg ml-1 Hygromycin B (Roche) or 50 µg ml-1 Kanamycin (ProGPT1-GPT1_N-long mat ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) containing cOmplete EDTA-free protein inhibitor cocktail (Roche, 1 tablet/10 ml), lysozyme (1.0 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... although the pancreas was injected with 1 mg/mL collagenase V (Roche, Indianapolis, USA), then digested for 16 min and centrifuged twice with Hanks Balanced Salt Solution ...
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bioRxiv - Cell Biology 2020Quote: ... and digested in HBSS medium containing 1.07 Wünsch units/ml of Liberase DH (Roche) and 600 IU of Hyaluronidase (Calbiochem ...
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bioRxiv - Cell Biology 2020Quote: ... the bile duct was cannulated and perfused with 2◻mL collagenase P (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated with DAPI (1 μg/mL) (Roche, cat # 10 236 276 001). Following multiple washes in PBS ...
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bioRxiv - Developmental Biology 2021Quote: Embryos were dissociated in 0.9x ice-cold PBS using 0.5mg per mL Liberase (Roche) then resuspended in 0.9x PBS/1% FCS ...
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bioRxiv - Microbiology 2021Quote: ... Magnetic beads were removed by magnet and 1mg/ml of anti-HA antibody (Roche) was added to the proteins for incubation overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and supernatant was treated with 1 μg/mL DNase I (Roche, Cat. No. 4716728001) for 2 hours at 37°C before sterilization by vacuum filtration through a 0.22 μM filter ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM NaF with 1 tablet/10 ml EDTA-free protease inhibitor cocktail tablets (Roche). Changes in global phosphorylation were analyzed as previously described (61) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1% sodium deoxycholate) with 10 µl/ mL of protease inhibitors prepared from Roche-cOmplete ...
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bioRxiv - Cell Biology 2022Quote: ... and 5% glycerol with protease inhibitors (Roche, 1 tablet per 10 mL lysis buffer). Worm slurry was frozen in liquid nitrogen and stored at -80 °C until lysis ...
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bioRxiv - Immunology 2021Quote: ... Lung was inflated with a digestion solution containing 1.5mg/ml of Collagenase A (Roche) and 0.4mg/ml DNaseI (Roche ...
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bioRxiv - Immunology 2021Quote: ... placed in DMEM with 30 µg/mL Liberase TM Research Grade (Roche Applied Science) and incubated at 37°C for 90 min (shaking).
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bioRxiv - Immunology 2019Quote: ... Skin was finely minced and then digested with 1 U/mL Liberase TL (Roche) + 0.5 mg/mL Hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... 500,000 cells from limb buds were then dissociated with 100ug/mL Liberase (Roche 05401119001), resuspended in CSKT buffer (10mM PIPES pH6.8 ...
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bioRxiv - Immunology 2020Quote: ... The cells were plated in pMCPECs media on 50ug/ml laminin (Roche, Mannheim, Germany) coated inverted filters of 5μm pore size and 6mm diameter (Corning ...
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bioRxiv - Pathology 2019Quote: ... and 200 μL of 20 mg/mL Proteinase K (Roche FFPET Isolation Kit # 06650775001) was added to each tube ...
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bioRxiv - Immunology 2020Quote: ... with the latter additionally subjected to digestion with 1 mg/mL Collagenase D (Roche) for 30 min at 37°C while shaken at 125 rpm ...
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bioRxiv - Molecular Biology 2019Quote: ... and washed with 50 mL 1 × PBS with complete™ Protease Inhibitor Cocktail (Roche). The cell pellet was snap frozen in liquid nitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Deparaffinized and rehydrated sections were treated with 8 μg/mL of Proteinase K (Roche) in PBS for 10 min ...
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bioRxiv - Genomics 2019Quote: ... Sections were placed in 4,6-diamidino-2-phenylindole (DAPI) (0.2 g /ml; 236276; Roche) for 10 minutes and then mounted on plus coated slides and coverslipped using Vectashield (H-1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with rat anti-HA antibodies (Roche, 3F10; 0.2 μg/ml) for 1 hr ...
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bioRxiv - Cell Biology 2019Quote: ... 1 cOmpleteTM mini EDTA-free protease inhibitor cocktail tab per 5 ml (Roche, 0463159001), 40 U RNaseOUTTM (Thermo Fisher 10777019 ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with cOmplete™ Protease Inhibitor Cocktail tablets (1/10 mL buffer; Roche, 11697498001). The suspended HLCs were placed on ice for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... the tissue was minced finely and digested with 0.28 units/ml Liberase TM (Roche) and 0.1 mg/ml of Deoxyribonuclease I (Worthington ...