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Citations for Roche :
4451 - 4500 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... cells were lysed in 1x RIPA buffer (Cell Signalling) with 1% PMSF and protease inhibitor cocktail (Roche). For gel-shift assays54 ...
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bioRxiv - Molecular Biology 2019Quote: ... The Bar amplified fragment (Table 1) labeled by DIG high primer DNA labeling (Roche, Cat. No. 11585614910) and purified using a high pure PCR product purification kit (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... and detected using an anti-DIG antibody coupled to alkaline phosphatase (1:5000, Roche, cat no. 11093274910) and NBT/BCIP staining (Roche ...
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bioRxiv - Cell Biology 2020Quote: Western blots were performed using the following antibodies: anti-myc antibody (Roche 0.4 mg/ml, 1/1000) followed by incubation with goat anti mouse HRP (BioRad 1/10000) ...
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bioRxiv - Microbiology 2020Quote: ... Pellets containing virions and EVs were resuspended in 1 ml PBS containing a protease cocktail inhibitor (Roche) during 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: A subcutaneous dose (1 or 2 mg/kg of weight) of diazepam (DZPM, Valium ®, Roche; México) or saline solution (SAL ...
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bioRxiv - Immunology 2021Quote: ... composed of 1 mg/mL collagenase D and 0.1 mg/mL DNase I (both from Roche, Switzerland) in complete RPMI medium (Gibco ...
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bioRxiv - Plant Biology 2021Quote: Tissues incubated with anti-KN1 were incubated in a 1:50 dilution of NBT/BCIP (Roche, #11681451001) in 0.05M MgCl2/TBS ...
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bioRxiv - Genomics 2021Quote: ... The supernatant was removed and the pellet was gently resolved in 200 μl Tween-Wash (0.1% Tween-20, 20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1 Roche Complete tablet-EDTA per 50 ml buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Slide were moved to a humid chamber and blocked with blocking solution (MAB 1×, blocking reagent (Roche) 2% ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.5) containing 10μg/ml of DNase 1 and complete Mini EDTA-free protease inhibitor tablets (Roche) (one tablet/10ml total volume) ...
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bioRxiv - Developmental Biology 2022Quote: ... or 1:4000 for anti-dig-alkaline phosphatase used in colorimetric in situ hybridizations (Roche, Basel Switzerland). For multiplex FISH ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM EDTA, 0.5 mM EGTA, 1% Triton x-100, 0.1% SDS, 0.1% sodium deoxycholate, 140 mM NaCl, Roche cOmplete Protease Inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: Mammary glands were dissected and incubated in a mixture of collagenase I (1 mg/ml, Roche Diagnostics) and hyaluronidase (50 μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated with 20 mU ml- 1 Vibrio cholerae-derived neuraminidase (sialidase) (#11080725001, Roche, Switzerland) for 1 h at 37°C on a cell shaker ...
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bioRxiv - Neuroscience 2022Quote: ... Following an overnight incubation at 4 °C with rat anti-HA (1:500, monoclonal, Roche, Cat # 11867423001) or mouse anti-GAPDH (1:25.000 ...
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bioRxiv - Microbiology 2022Quote: ... Detection was done by incubation with 1 ml of detection buffer containing 100 μL CDP-Star (Roche) and exposure in a Gel Doc.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then collected through centrifugation and resuspended in 1 mL PBS supplemented with complete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Plant Biology 2022Quote: ... For every biological replicate 500 μg protein was digested with sequencing grade trypsin (1:100 trypsin:protein; Roche) overnight at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were then resuspended in 1000 µL cold nucleoplasm extraction buffer (50 mM HEPES pH 7.9, 150 mM K Acetate, 1.5 mM MgCl2, 0.1% [vol/vol] Nonidet P40, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Molecular Biology 2022Quote: Radioimmunoprecipitation Assay (RIPA) buffer and cOmplete mini-proteinase inhibitors (1 tablet per 10 ml extraction solution, Roche) was used to homogenize striatal tissue and cells ...
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bioRxiv - Molecular Biology 2022Quote: ... the crushed liver was digested with a solution containing 1% DNase (10104159001, Roche Diagnostics GmbH, Mannheim, Germany), 0.5 mg/mL protease ...
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bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed in blocking buffer with anti-HA-tag (rat, Roche, 11867423001, 1:250) or anti-CD9 (mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... RNaseA treated samples were then mixed with 1 μL of 19 mg/ml Proteinase K solution (Roche) and incubated at 55 °C for overnight ...
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bioRxiv - Biochemistry 2024Quote: ... 10% (v/v) glycerol and 1× Halt or 1x cOmpleteTM EDTA-free protease inhibitor cocktail (Roche #11873580001). Lysates were sonicated with a probe sonicator and cleared via centrifugation at 15,000x g for 5 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... all tissue sections were stained with DAPI (4’, 6-Diamidine-2’-phenylindole dihydrochloride, #10236276001, Roche, 1:3000). Images were taken with a confocal microscope from Zeiss (LSM 880 Airyscan).
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT, 0.15% NP40, 1 x Complete protease inhibitors, Roche) and rotated at 4 °C for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were incubated with 10 mL of ice-cold buffer A (10 mM Hepes-KOH pH 7.9, 1.5 mM MgCl2,10 mM KCl, 1 mM DTT, 1x Complete Protease inhibitors, Roche) for 1–2 min ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted using 1xPBS pH 7.4 with Protease Inhibitor Cocktail (1 tablet/10 mL) (Roche Diagnostics) and sonicated during 10 seconds at 10% power ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA produced was diluted 1:2 for use with LightCycler 480 SYBR Green I Master (Roche) with the primers listed in the section above ...
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bioRxiv - Developmental Biology 2024Quote: ... the antibody incubation was performed with 1:5000 diluted Anti-Dig-AP antibody (Roche, Cat. No. 11093274910) at 4°C overnight ...
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bioRxiv - Physiology 2024Quote: ... and incubated in the staining buffer containing VHH15-mCherry (1:30 dilution) and protease inhibitor cocktail (Roche) in Krebs buffer for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The MHCII primary was of mouse origin ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies used for IFA were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:200) (Roche); rabbit anti-GAP45 mAb (1:1000).
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bioRxiv - Cell Biology 2023Quote: ... all tissue sections were stained with DAPI (4′, 6-Diamidine-2′-phenylindole dihydrochloride, #10236276001, Roche, 1:3000). For image generation an Axio Observer ...
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bioRxiv - Genomics 2023Quote: ... The blocking solution was then replaced with antibody solution (buffer B2 containing DIG-antibody 1:26,000 (Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 400 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.4% [vol/vol] Nonidet P40, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... a portion of spleens was digested on a solution containing 0.2 mg ml−1 collagenase P (Roche), 0.1 mg ml−1 DNAase I in RPMI-1640 medium and enriched by depleting CD45+ cell and TER119+ cells using MACS microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of RNA was used to synthesize cDNA with First Strand cDNA Synthesis kit (Roche, IN). The reaction master mix contained EvaGreen polymerase (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2023Quote: ... Both kidneys were incubated in 10 ml enzymatic solution (1 mg/ml collagenase A (Roche Diagnostics, Germany), 2.5 mg/ml trypsin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% Triton X-100) with added 72 mM ß-mercaptoethanol and 1× complete protease inhibitor cocktail (Roche)45 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... For NBT/BCIP staining sections were incubated overnight with sheep anti-DIG-AP (1:1000, Roche 11093274910). After washing staining was performed using NBT/BCIP in NTMT until satisfactory intensity ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: • PCR #1: 10 μl reactions were prepared for each sample with KAPA HiFi HotStart ReadyMix (Roche, KK2602) containing 1 μl cDNA template and 1.5 μl of 10 μM PCR1 primer mix (U6 outer ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue fragments were then digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation and cell death were assessed using the WST-1 and cytotoxicity Detection KitPLUS (LDH) (Roche) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting pellet was resuspended into 1500 µl nuclei suspension buffer (NSB, 0.5 % BSA, 1:100 Roche protease inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Washed bladders were incubated for 1 h at 37 °C in 0.34 U/mL of Liberase (Roche) in PBS ...