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Citations for Roche :
4251 - 4300 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Contaminating DNA was digested by DNase I (Roche; 1 U/µg RNA, 60 min, 37°C) in the presence of RNase inhibitor (RNaseOUT ...
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bioRxiv - Developmental Biology 2019Quote: ... Probes were detected by over-night incubation with anti-Digoxigenin-AP Fab fragments (1:5000) (Roche) and stained with standard Nitro Blue Tetrazolium (NBT ...
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bioRxiv - Genomics 2021Quote: ... Second-stage digestion of SUMOylated peptides was performed with 1 μg of Endoproteinase Asp-N (Roche). Digestion was performed overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 6xHis-HA-RGL2-6His and its ubiquitinated conjugates were detected using anti-HA (1:1000; Roche) antibody.
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 and 2 mM phenylmethylsulfonyl fluoride and Complete Protease Inhibitor cocktail (Roche Diagnostics). 2 µg of the extracted proteins were separated by SDS-PAGE gel and stained with CBB R-250 ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM KCl containing 1% (w/v) digitonin and 1X Protease Inhibitor mix (Roche, EDTA-free). After centrifugation (15 min ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM KCl containing 1% (w/v) digitonin and 1X Protease Inhibitor mix (Roche, EDTA-free), or by sonication on ice (3x 10s ...
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bioRxiv - Cell Biology 2021Quote: ... On Day 0 (start of differentiation) hPSCs were treated with 1 mg/ml Collagenase B (Roche) for one hour ...
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bioRxiv - Immunology 2021Quote: ... washed and rested for 1 h in 0.25 µL/mL DNase I (Roche Diagnostics, Indianapolis, IN)-containing R-10 complete medium (RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... skeletal muscle tissue was enzymatically digested with 1.5 U mL−1 collagenase D (Roche, Ref. 11088882001), 2.4 U mL−1 dispase II (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and digested in 2.5 ml of culture media containing 1 mg/mL of Collagenase IV (Roche) and 50 unit/mL DNase (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-POD antibodies (1:500, Roche) and revealed using the kit TSA-Plus Cyanin-3 (Perkin Elmer).
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bioRxiv - Immunology 2020Quote: ... The bacterial pellet was resuspended in 1 ml of PBS containing protease inhibitors (cOmplete Mini, Roche; 1 tablet per 10 ml of PBS following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Digoxigenin FISH signals were amplified using sheep anti-digoxigenin fluorescein fab fragments (1:50, Roche 11207741910) and fluorescein rabbit anti-sheep antibodies (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS)) supplemented with 1X protease inhibitor cocktail (Complete; Roche), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Immunology 2020Quote: ... 10% glycerol and 1% Triton X-100) supplemented with a protease and phosphatase inhibitor mixture (Roche) for 20min on ice ...
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bioRxiv - Genetics 2019Quote: ... Cells were scraped in PBS supplemented with protease inhibitor cocktail (1:100, 11836145001, Roche applied science) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM AEBSF and 100U/mL of RNasin) supplemented with protease cocktail inhibitor EDTA-free (Roche), on ice for 20 min ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Cell lysates (1 mg) were pre-cleared with Protein-G–agarose beads (Roche, catalog number 11719416001) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween20) before color was developed via addition of 1:100 NBT/BCIP solution (Roche, 11681451001) in staining solution ...
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bioRxiv - Cell Biology 2022Quote: ... The levels of IR were analyzed by western blotting (Anti-Myc; WB, 1:2000; 9E10, Roche).
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bioRxiv - Genomics 2022Quote: Commercially available or published antibodies used for western blotting: rat anti- HA (1:2000, Roche 11867423001) (Figure 9A,B) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% sodium deoxycholate) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitor cocktail (Roche)) on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... The ARS305 probe was generated by PCR using primers ARS305_probe_F and ARS305_probe_R (Table 1) and the PCR DIG Labeling Mix (Roche). Membranes were imaged using an Azure c600 chemiluminescence Imaging System.
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bioRxiv - Bioengineering 2022Quote: ... by incubating for 30 minutes followed by incubating with DAPI (Roche, Cat. # 10236276001, 1:1000 dilution) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... and stored at least 1 hour at −20 °C before staining with Tdt reaction mix (Roche Diagnostics ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) supplemented with 0.3 mg.mL-1 lysozyme and EDTA-free protease inhibitor cocktail (Roche) prior to pressure-assisted lysis using a French press system ...
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bioRxiv - Biophysics 2022Quote: ... 25 mM EDTA and Complete Protease Inhibitor Cocktail tablets (Roche, 1 tablet per 50 ml buffer). The mixture was homogenized and then passed twice through a microfluidizer ...
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bioRxiv - Cell Biology 2022Quote: ... muscles were mechanically disaggregated and dissociated in DMEM 1% P/S media containing collagenase B (Roche) 0.2% and Calcium dichloride (CaCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were incubated with horseradish peroxidase (HRP) conjugated sheep anti-digoxigenin antibody (1:100, Roche) overnight at RT ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies used (at 1/1,000, unless specified) were rat anti-HA tag (clone 3F10, Roche), mouse anti-TY tag (27) ...
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bioRxiv - Microbiology 2022Quote: ... and 1% NP-40) supplemented with cOmplete protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail (Roche). After centrifugation at 20,000 × g for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: Tumor draining lymph nodes (tdLNs) were minced and digested with Collagenase D (1 mg/mL, Roche) and DNAse I (50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 1% SDS) supplemented with a protease inhibitor cocktail (Complete Mini-EDTA free from Roche, Penzberg, Germany), and then centrifuged ...
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bioRxiv - Neuroscience 2022Quote: ... Following 1 hour incubation in blocking buffer (10% lamb serum, 0.2% Roche blocking reagent in TBS), the slides were incubated overnight at 4°C in HRP-conjugated anti-DIG or anti-FL antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: rat anti HA (1:500, Roche, #11-867-423-001), chicken anti GFP (1:800 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche) and analyzed using western blots.
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease inhibitors (1 mM PMSF and 1x PIC - Roche). Cell lysates were passed through a 1 ml syringe for mechanical disruption and centrifuged for 10 min at 13.000 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked for 1 hour at room temperature with 5% blocking agent (Roche Diagnostic) in TBST (100 rpm shaker) ...
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bioRxiv - Neuroscience 2022Quote: ... Complete mini protease inhibitor mixture (1 tablet in 10 ml of distilled water; Roche Indianapolis, IN) and phosphatase inhibitors (20 mM NaF and 0.5 mM sodium orthovanadate) ...
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bioRxiv - Plant Biology 2022Quote: ... with Complete Ultra EDTA-free Protease Inhibitor Cocktail Tablets (Roche, Switzerland; 1 tablet per 10 mL)] ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated for 30 mins in 1:5000 Anti-Digoxigenin- AP Fab Fragment antibodies (Roche; 11093274910). After washing the membrane twice in buffer 1 for 15 mins ...
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bioRxiv - Microbiology 2022Quote: ... and 1% Igepal CA-630) containing a 1X protease inhibitor cocktail (cOmplete Mini EDTA-Free, Roche). We separated proteins by sodium dodecyl sulfate (SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween-20) and developed with a 1:50 dilution of NBT/BCIP solution (Roche #11681451001) in NTMT at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... lymph nodes were manually minced into pieces and digested with collagenase A (1 mg/mL; Roche) and DNAse (10 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... 300 mM NaCl and 1 mM cOmplete™ protease inhibitor cocktail (Roche Molecular Systems, Inc., USA). The obtained sample was loaded in a Ni-NTA affinity chromatography column (Sigma Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondrial dehydrogenase activity was assayed using the WST-1 reagent (Roche Applied Science Ltd, Hertfordshire, UK) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with a 1:5000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche 11093274910) in MABT/Block overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM imidazole] containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease inhibitor cocktail (Complete™; Roche), and recombinant proteins were purified by immobilized metal affinity chromatography (TALON ...