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Citations for Roche :
4501 - 4550 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation and cell death were assessed using the WST-1 and cytotoxicity Detection KitPLUS (LDH) (Roche) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
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bioRxiv - Immunology 2023Quote: ... Washed bladders were incubated for 1 h at 37 °C in 0.34 U/mL of Liberase (Roche) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Bead-bound cells were resuspended in 50 mL buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM Spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... mechanically disrupted by a tissue homogenizer (Ika) in 1 ml of PBS with protease inhibitor cocktail (Roche) and spun at 10,000 x g for 10 min to pellet debris ...
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bioRxiv - Biochemistry 2022Quote: ... then resuspended in lysis buffer supplemented with complete protease inhibitor cocktail tablets (Roche, 1 tablet per 50ml) at 0.3 x pellet volume ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... in deionized phosphate-buffered saline (PBS)] supplemented with 1:7 proteases inhibitors cocktail (Roche Diagnostics GmBH, Germany) for 10 min ...
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bioRxiv - Genetics 2023Quote: ... 1 Complete EDTA-free Protease Inhibitor cocktail tablet per 12 mL lysis buffer (Roche Applied Science, #1873580)] and drop frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The SARS-CoV-2 spike primary antibody was of rabbit origin ...
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bioRxiv - Immunology 2023Quote: ... were lysed in 100 µL or 300 mL RIPA buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1% IGEPAL CA-630, 0.25% Na-deoxycholate, 2 mM EDTA, 0.1% SDS, Roche cOmplete™ Mini protease Inhibitor Cocktail) ...
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bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) containing protease and phosphatase inhibitors (cOmplete™ and PhosTOP™, Roche/Merck, Dorset, UK), 1 mM sodium orthovanadate and 1 mM phenylmethylsulfonyl fluoride and incubated for 30 min on ice.
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bioRxiv - Cell Biology 2023Quote: ... or Nunc Glass Base Dishes (Thermo Fischer Scientific) coated with sterile 10 µg ml−1 fibronectin (Roche). Supplier and identifier for all reagents are listed in Table 4 ...
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES pH 7.6) supplemented with 1% SDS and protease inhibitors (EDTA-free protease inhibitor cocktail; Roche) and transferred to 15 mL polystyrene Falcon tubes compatible with sonication ...
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bioRxiv - Neuroscience 2023Quote: ... in lysis buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, complete protease inhibitors, Roche) and 4 U per ml Turbo-DNase (Thermofisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Bead-bound cells were resuspended in 50 ml buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 3 hours with Anti-Digoxigenin-AP Fab fragments (dilution 1:3000) (Roche, 11093274910) at room temperature ...
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bioRxiv - Genomics 2023Quote: ... The blocking solution was then replaced with antibody solution (buffer B2 containing DIG-antibody 1:26,000 (Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... washed with PBS and permeabilised at 1 × 107 cells/ml in MELB with cOmplete protease inhibitors (Roche) and 0.025% digitonin (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using a Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... all tissue sections were stained with DAPI (4′, 6-Diamidine-2′-phenylindole dihydrochloride, #10236276001, Roche, 1:3000). For image generation an Axio Observer ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in lysis buffer containing 1% Nonidet P-40 and protease inhibitor cocktail (Roche) (Cao ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue fragments were then digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein blots were probed with anti-HA antibodies conjugated to horseradish peroxidase (Roche, Switzerland, 12013819001, 1:4000). Immunoblots were visualised with Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and digested for 45 to 60 minutes in a solution of collagenase A (1 mg/mL) (Roche) and DNAse I ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ml TLES buffer (TLE + 0.1% (w/v) SDS) with 100 μg/ml proteinase K (Roche, #03115879001) was added and the suspension was incubated at 37°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5) supplemented with 1 mM PMSF and cOmplete protease inhibitor cocktail (EDTA-free) (Roche, cat. # 11836170001). One gram of glass beads with a diameter of 0.5 mm (YASUI KIKAI ...
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bioRxiv - Neuroscience 2024Quote: ... and 100,000 worms were collected in 1 ml of RIPA buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... 1ml of CDP-Star® Chemiluminescent Substrate (Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate) (Roche, Cat No. 11685627001) was added to 9ml of DIG-detection buffer and membranes were then incubated with the substrate for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... The dissected tumor samples were placed in RPMI with 1.5 U mL-1 Liberase TM (Roche 5401119001), 0.2 mg mL-1 DNase I (Worthington Biochemical LS002006 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed twice more in PBS and stained with 1 μg/mL DAPI (Roche, 10236276001) in PBS for 5 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: IPSC-derived dopaminergic neurons were lysed in 1% SDS supplemented with protease inhibitors (Roche, cat no. 11697498001) and immediately boiled at 95°C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM KCl, 0.1% NP-40, 0.2 mM PMSF, 0.2mM benzamidine, 1× EDTA-free protease inhibitor cocktail [Roche]). Following clarification by two rounds of centrifugation ...
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bioRxiv - Immunology 2024Quote: ... intestinal pieces were digested in 5% FBS medium (RPMI) supplemented with 1 mg/ml collagenase D (Roche) and 0.5 mg/ml DNase I (Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 mM Tris(2-carboxyethyl)phosphine (TCEP) and protease inhibitor (Complete EDTA-free Protease Inhibitor Cocktail, Roche)) for purification ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was probed using the primary antibodies mAb rat α-HA (1:2,000, Roche Diagnostics #11867423001) or mAb mouse α-PfGAPDH (1:20,000 ...
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bioRxiv - Genetics 2024Quote: ... The dried pellets were resuspended in 200 µl of lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 2.75 mM DTT, 1.1 mM PMSF, Roche protease inhibitor at 2x working concentration (Roche 04693132001)) ...
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bioRxiv - Microbiology 2024Quote: ... 1% triton 100X) supplemented with 0.1 mg/mL lysozyme and complete EDTA-free Protease Inhibitor Cocktail (Roche) for 1 hour on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated into single cells by treatment with 1 mg/ml Dispase II (Roche, 04942 078001) and 2.5 mg/ml collagenase I (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... Suspended membrane vesicles were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... The trypsin digestion was quenched by adding 4 μL of 1× EDTA-free protease inhibitor cocktail (Roche 11873580001). The supernatants were collected and the beads washed (50μL ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.65% Nonidet P-40 and 1 mM phenylmethylsulfonyl fluoride) supplemented with proteinase inhibitor mixture (cOmpleteTM EDTA-free; Roche). The nuclei were harvested by scraping into a 1.5-ml microcentrifuge tube and collected by centrifugation at 500 × g for 5 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the membrane was first probed with an anti-GFP antibody (Cat.No. 11814460001, Roche, Basel, Switzerland; 1:3’000 dilution) followed by a secondary anti-mouse-IR800 (R-05061 ...
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bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated at room-temperature with a 1:500 dilution of anti-Dioxigenin-AP Fab fragments (Roche, #11093274910) in 1% BSA/0.3 % Triton-X100/TBS for 1hour ...