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Citations for Roche :
401 - 450 of 3695 citations for ssc mir 487b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... then precipitated and cleaned before resuspension in hybridization buffer (65% formamide, 5X SSC, 5X Denhardts with 1% blocking reagent (Roche), 0.5 mg/mL salmon sperm DNA added fresh) ...
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bioRxiv - Genetics 2022Quote: ... Samples were blocked for 30 minutes in a solution of 1% BSA in 2x SSC with 0.1% tween at room temperature and then rhodamine-conjugated anti-digoxigenin (Roche, #11207750910) or FITC-conjugated anti-biotin (AbCam ...
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bioRxiv - Developmental Biology 2021Quote: ... 5X SSC, 100 µg/ml heparin, 100 µg/ml yeast RNA, 0.1% TritonX-100, 0.1% CHAPS, 2% Roche blocking reagent) for more than an hour at 60–65 °C ...
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bioRxiv - Genomics 2020Quote: ... Sections were then washed several times with SSC of increasing stringency and then incubated with alkaline phosphatase-conjugated anti-digoxigenin antibodies (Roche). Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with high stringency (0.5× SSC, 70°C) and developed with alkaline phosphatase-conjugated anti-DIG antibody (Roche, Switzerland) with CDP-Star substrate in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
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bioRxiv - Genetics 2021Quote: ... The RT-PCR reaction was carried out in a Light Cycler 480 fluorescence quantitative meter (Fritz Hoffmann-La Roche Co. Ltd., Basel, Switzerland).
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bioRxiv - Plant Biology 2019Quote: ... were used as template for quantitative real time RT-PCRs which were performed in 384-well plates using a LightCycler 480 II detection system (Roche Diagnostics, Mannheim, Germany). GoTaq PCR Mastermix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping was conducted by real-time polymerase chain reaction (RT-PCR) and subsequent high resolution high-resolution melting on a Cobas Z480 Light Cycler (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x) Kit (Kapa Biosystems, Cape Town, South Africa) in LightCycler 480® (Roche ...
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bioRxiv - Genomics 2020Quote: ... and the DIG Wash and Block Buffer Set (Roche 11585762001), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and the DIG Wash and Block Buffer Set (Roche 11585762001). 5 to 10 ug of genomic DNA (roughly genomic DNA extracted from about 15 adult flies ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2 μM of both reverse and forward primers and the PCRs were run on a Roche Lightcycler 480 thermocycler (Roche Applied Science, Basel, Switzerland). Each sample and primer pair was run in triplicates ...
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bioRxiv - Genetics 2022Quote: ... and amplifying the exon regions of targeted genes by using the primers with SP6 sequence (ATTTAGGTGACACTATAGAAGNG) according to the product description of KAPA HiFi PCR Kit (Roche Diagnostics, cat. no. 07958927001). Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche ...
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bioRxiv - Immunology 2022Quote: ... with oligo-dT primers and then amplified them by 22 cycles of PCR using the KAPA HiFi Hot Start Ready Mix (Kapa Biosystems, Wilmington, MA, USA) with 5Me-isodC-TSO and ISPCR primers ...
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bioRxiv - Developmental Biology 2022Quote: ... 5x SSC, 0.05mg/ml Heparin, 0.25% Tween20, 1% SDS, 1 mg/ml Salmon Sperm DNA, 1 mg/ml Roche blocking buffer powder) containing oligo probes at a concentration of 1μg/120μl and hybridized for two days at 43°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were gradually washed into SSC buffer and then into PBT before overnight incubation with an anti-DIG primary antibody at 1:5000 (Roche #11093274910). Embryos were washed in PBT and then staining buffer before developing in BM Purple staining solution (Roche #11442074001) ...
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bioRxiv - Immunology 2019Quote: ... MicroRNA RT-qPCR was performed with ExiLENT SYBR® Green master mix (Exiqon) using LightCycler ® 96 Real-Time PCR System (Roche Life Science). Primers used in this study are ...
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bioRxiv - Immunology 2019Quote: ... RT-PCR was performed on the LightCycler® 480 instrument using the LightCycler® 480 SYBR Green I Master mix (Roche Diagnostics, Mannheim, Germany) and oligonucleotides listed in Table 1 ...
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bioRxiv - Genomics 2021Quote: ... Quantification of final library before loading on MiSeq sequencer was performed using the Kapa quantification kit (RT-PCR) for next generation sequencing with the Illumina platform (Kapa Biosystems, Wilmington, MA USA). Sequence reads were quality filtered and assembled using CLC Genomics Workbench 8.0.1 (CLCbio ...
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bioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA synthesis and RT-qPCR was carried out using One step RT QPCR reagent (Roche). Resulting signal was detected on Applied Biosystems Real Time PCR System ...
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bioRxiv - Neuroscience 2019Quote: ... with random hexamer primers (Roche Diagnostics, Germany) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... using random primers p(dN)6 (Roche). Quantitative PCR reactions were performed in a BioRad CFX96 system employing the SYBRgreen fluorescent reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... All probes and primers were from Roche or Thermofisher.
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bioRxiv - Neuroscience 2020Quote: ... using random primers p(dN)6 (Roche). Quantitative PCR reactions were performed using standard protocols using EvaGreenTM in the Stratagene Mx3000P system (Agilent Technologies) ...
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bioRxiv - Pathology 2022Quote: ... All probes and primers were from Roche. Primer sequences:
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bioRxiv - Developmental Biology 2023Quote: ... and 500 ng of random primer (Roche) at 25°C for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µM random hexamer primers (Roche # 11034731001) and 1 μl RNAsin Plus Inhibitor (Promega #N2615 ...
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bioRxiv - Microbiology 2023Quote: ... using random primers p(dN)6 (Roche). Quantitative real-time PCR was performed on a real-time PCR system (Quant Studio 6 Flex ...
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bioRxiv - Cell Biology 2024Quote: ... using random primers p(dN)6 (Roche). Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Microbiology 2020Quote: ... Thermocycling conditions were set on a LightCycler® 96 Instrument (Roche), as follows ...
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bioRxiv - Molecular Biology 2019Quote: ... and ligated to KAPA single indexed adapters Set B (KK8702, Roche). Samples were processed according to manufacturer’s instructions with one exception ...
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bioRxiv - Developmental Biology 2020Quote: ... Reaction was set up with KAPA SYBR® FAST Universal (Roche) in a total volume of 10 µl ...
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bioRxiv - Microbiology 2021Quote: ... and detected with DIG wash and block buffer set (Roche-11585762001).
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bioRxiv - Bioengineering 2023Quote: ... of the VH gene was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene-specific primers (primer No. 23–32, 7.5 μl) and a KAPA Biosystems kit (Roche). The reaction conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... separated RNA was transferred to positively charged nylon membranes using overnight capillary transfer in 20X saline-sodium citrate (SSC) buffer as described in the DIG Northern Starter Kit manual (Roche, Cat. 12039672910).
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bioRxiv - Neuroscience 2019Quote: ... samples were pretreated with hybridization buffer (50% formaldehyde, 5 × SSC, 0.1% tween, 100 ug/ml tRNA [Roche], 50 ug/ml heparin [Sigma]) for 2 hr at 65°C ...
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bioRxiv - Microbiology 2023Quote: ... where SARS-CoV-2 RNA was detected by real time reverse transcriptase real time polymerase chain reaction (RT-PCR) one either a Cobas® 6800 (Roche Diagnostics GmbH (Mannheim, Germany)) ...
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bioRxiv - Cell Biology 2019Quote: ... 4 μl 5xTranscriptor RT reaction buffer (Roche), 0.5 μl RNase OUT (Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR reactions on a LightCycler480 (Roche) to probe for the genes of interest (see Supplementary Table 1 for primer sequences) ...
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bioRxiv - Microbiology 2023Quote: ... no-chloroplast amplifying primers 335F (CADACTCCTACGGGAGGC) and 769R (ATCCTGTTTGMTMCCCVCRC) (Dorn-In et al. 2015) were used together with KAPA2G Robust PCR Kits (Kapa Biosystems, Cat. No. KK5005, Wilmington, USA). In protocol 2 (P2) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences are shown in Table S3) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences shown in table S2) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Probe was prepared by PCR amplification using forward primers LL/F 5′-TACGGACACAGGTCGAATCCCCTACTACC-3′ and reverse primer LL/R 5′-ACAGAGAAGAGGCTAATGTGTGCAC-3′ in the presence of DIG (Roche). PCR-products were resolved in a 1.2 % agarose Ethidium bromide-stained gel ...
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bioRxiv - Genomics 2023Quote: ... 100ng of library were amplified using an upstream (US) forward primer and a universal reverse primer using the KAPA2G Robust HotStart ReadyMix (Roche). The resulting product was amplified using a downstream (DS ...
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bioRxiv - Cancer Biology 2019Quote: ... and specific primers on a LightCycler 480 (Roche). The reactions were performed in triplicates ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using random primers (Roche, #11034731001). Samples were amplified for 35 cycles using the 7500 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was produced with random hexameric primers (Roche) using M-MLV (Invitrogen ...