Labshake search
Citations for Roche :
4301 - 4350 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation and resuspended in 0.3 ml PBS supplemented with complete mini protease Inhibitor Cocktail (Roche). Whole cell protein extracts were obtained as described above and protein concentrations determined using Bradford reagent (Thermo Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... Washed bladders were incubated for 1 h at 37 °C in 0.34 U/mL of Liberase (Roche) in PBS ...
-
bioRxiv - Microbiology 2023Quote: ... and the cell suspension were digested in HBSS containg 25 μg /ml of DNAse I (#11284932001, Roche) and 50 μg/ml Liberase (#5401119001 ...
-
bioRxiv - Molecular Biology 2023Quote: ... in the smallest volume of ice-cold 1X PBS - 0,6% D-glucose - 5mg/ml DNaseI (Roche #10104159001) solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bead-bound cells were resuspended in 50 mL buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM Spermidine, 1× Roche complete protease inhibitors ...
-
bioRxiv - Microbiology 2023Quote: ... mechanically disrupted by a tissue homogenizer (Ika) in 1 ml of PBS with protease inhibitor cocktail (Roche) and spun at 10,000 x g for 10 min to pellet debris ...
-
bioRxiv - Genomics 2023Quote: ... 1 M sodium phosphate (pH 7.2) and 0.5 ml of sonicated fish sperm solution (Roche Diagnostics, Switzerland). Probes were labeled with 32P by nick translation (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: Adult rat ventricular cardiomyocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in modified Krebs-Henseleit buffer (135mM NaCl ...
-
bioRxiv - Genetics 2023Quote: ... 1 Complete EDTA-free Protease Inhibitor cocktail tablet per 12 mL lysis buffer (Roche Applied Science, #1873580)] and drop frozen in liquid nitrogen ...
-
bioRxiv - Immunology 2023Quote: ... The pellet was resuspended in 3.0 ml of PBS containing a complete Mini Protease Inhibitor tablet (Roche), split into two eppendorf tubes prefilled with 150 μl of 0.5-mm glass beads (Scientific Industries ...
-
bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
-
bioRxiv - Cancer Biology 2023Quote: ... + 4% fetal bovine serum (FBS, Hyclone) and 0.01 mg/mL insulin-transferrin-sodium-selenite solution (ITSS; Roche) as previously described 28,29 ...
-
bioRxiv - Cell Biology 2023Quote: ... or Nunc Glass Base Dishes (Thermo Fischer Scientific) coated with sterile 10 µg ml−1 fibronectin (Roche). Supplier and identifier for all reagents are listed in Table 4 ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.5 ml ice-cold 8 % (v/v) paraformaldehyde solution in vPBS containing EDTA-free protease inhibitor (Roche) or ice-cold 8% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: ... Bead-bound cells were resuspended in 50 ml buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1× Roche complete protease inhibitors ...
-
bioRxiv - Cell Biology 2023Quote: ... washed with PBS and permeabilised at 1 × 107 cells/ml in MELB with cOmplete protease inhibitors (Roche) and 0.025% digitonin (w/v ...
-
bioRxiv - Immunology 2023Quote: ... The tissue was minced finely in the digest containing RPMI with 0.1 mg/ml liberase CI (Roche) and 1mg/ml DNase I (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... after cutting SVF into small pieces and incubation in a 2 mg/ml collagenase D solution (Roche) at 37°C in a water bath for 45 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and digested for 45 to 60 minutes in a solution of collagenase A (1 mg/mL) (Roche) and DNAse I ...
-
bioRxiv - Immunology 2024Quote: Lymph nodes were mechanically disrupted with tweezers and incubated in RPMI with 0.8mg/mL Dispase II (Roche), 0.2mg/mL Collagenase P (Roche) ...
-
bioRxiv - Microbiology 2024Quote: ... 1% triton 100X) supplemented with 0.1 mg/mL lysozyme and complete EDTA-free Protease Inhibitor Cocktail (Roche) for 1 hour on ice ...
-
bioRxiv - Cell Biology 2024Quote: ... Oocytes were isolated from the ovary and defolliculated with 1.0 to 1.5 mg/mL collagenase A (Roche) in OR2 buffer (82 mM NaCl ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then washed twice more in PBS and stained with 1 μg/mL DAPI (Roche, 10236276001) in PBS for 5 min at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Lungs were homogenized with 1 mm silica beads in 1 mL PBS using a MagNA Lyser (Roche). Homogenization was carried out at 6,000 rpm for 60 s and chilled on ice for 5 min in-between runs ...
-
bioRxiv - Immunology 2024Quote: ... intestinal pieces were digested in 5% FBS medium (RPMI) supplemented with 1 mg/ml collagenase D (Roche) and 0.5 mg/ml DNase I (Sigma ...
-
bioRxiv - Neuroscience 2024Quote: ... The minced tissues after the centrifuge were incubated with collagenase IV (2.5 mg/ml, ROCHE, Cat# 11088858001) at 37 °C with gentle rotation for 30 min in 2 ml of HBSS++ ...
-
bioRxiv - Microbiology 2024Quote: ... The dissected tumor samples were placed in RPMI with 1.5 U mL-1 Liberase TM (Roche 5401119001), 0.2 mg mL-1 DNase I (Worthington Biochemical LS002006 ...
-
bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated into single cells by treatment with 1 mg/ml Dispase II (Roche, 04942 078001) and 2.5 mg/ml collagenase I (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... Nematodes were resuspended in 8 M urea in 100 mM TRIS-HCl buffer (pH 8) containing cOmplete™ protease inhibitor cocktails (Roche Diagnostic Canada, Laval, QC, Canada) and aliquoted into reinforced 1.5 mL homogenizer tubes containing 500 μm glass bead homogenizer tubes with 25 mg of glass beads ...
-
bioRxiv - Developmental Biology 2019Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lavroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). The sample was then sonicated using a Covaris S220 at the following settings ...
-
bioRxiv - Developmental Biology 2022Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lauroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). For each IP experiment ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 mM MgCl2, 1% Triton X-100, 50 mM HEPES pH 7.4, 1 x Protease Inhibitor Cocktail [04693159001, Roche Diagnostics], 50 mM NaF, 0.2 mM Na3VO4). The protein concentration was determined by BCA assay (23225 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 2 mM MgCl2, 1% Triton X-100, 50 mM HEPES pH 7.4, 1 × Protease Inhibitor Cocktail [04693159001, Roche Diagnostics], 50 mM NaF, 0.2 mM Na3VO4). The remaining (87% ...
-
bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed using 0.4 mL lysis buffer containing 2x protease inhibitors (Complete, Mini, EDTA-free; Roche), 1x phosphatase inhibitors (PhosStop ...
-
bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The nuclei were resuspended at 1 million nuclei per mL in wash buffer (20mM HEPES pH 7.9, 150mM NaCl, 0.5mM Spermidine supplemented with 1x Roche cOmplete mini ...
-
bioRxiv - Physiology 2022Quote: ... Digestion was continued by adding 200 μL of LiberaseTM TL Research Grade (0.25 mg/mL; Hoffmann-La Roche) per 2 mL of cell isolation solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were washed three times on the magnet in 1 mL buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
-
bioRxiv - Immunology 2019Quote: ... 50 mM KCl and 0.45% Tergitol at pH 8.0) that was freshly supplemented with 1 μg/mL proteinase K (Roche). After deactivating proteinase K by incubating for 15min at 95 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to PVDF membranes and labelled overnight with anti-HA (0.01 μg/mL, Roche clone 3F10), anti-VE-Cadherin (0.5 μg.ml−1 ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were lysed with lysis buffer (final concentration: 50 µg/ml lysozyme, 0.8% NP40, 1X protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
-
bioRxiv - Genomics 2019Quote: ... Cell nuclei pellet was re-suspended in 2 mL FACS buffer (0.15 mM spermine, 0.5 mM spermidine, Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma ...
-
bioRxiv - Developmental Biology 2021Quote: ... Fixed embryos were placed into hybridization buffer (50% formamide, 5×SSC, 1 mg/ml yeast tRNA (Roche, 10109223001), 100 μg/ml heparin (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
-
bioRxiv - Immunology 2021Quote: ... Individual intact lung lobes were cut away and additionally kept in a dispase solution (50 U/ml, Roche) for 45 min at room temperature on a rocker at 150 rpm ...
-
bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche] ...
-
bioRxiv - Neuroscience 2021Quote: ... Trypsin was aspirated and the digestion was stopped by adding 0.5 mg/mL DNase I (Roche, Basel, Switzerland) in microglia medium [Dulbecco’s modified Eagle’s medium (DMEM ...