Labshake search
Citations for Roche :
4101 - 4150 of 5469 citations for Rat BMP7 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was fragmented to 100-300bp and libraries were constructed using the KAPA HTP Library Kits (KAPA Biosystems). A single low input sample used the Swift Accel-NGS 2S DNA Library Kit (Swift BioSciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative RT-PCR for library quantification was performed using the Kapa Library Quantification Kit (Roche Sequencing, Pleasanton, CA). The libraries were sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate about 40 million read pairs per sample.
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bioRxiv - Genomics 2019Quote: All 5’ fragments were amplified off the array using HSSF-ATGC and DO_15R_PU (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) and stopped before plateauing ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared using a miniaturized version of the Kapa HyperPlus Illumina-compatible library prep kit (Kapa Biosystems). DNA extracts were normalized to 5 ng total input per sample using an Echo 550 acoustic liquid handling robot (Labcyte Inc) ...
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bioRxiv - Microbiology 2021Quote: ... shotgun library construction was performed for the extracted DNA using a KAPA Hyper Prep kit for Illumina (KAPA Biosystems), and sequencing of the library was performed on an Illumina MiSeq platform (MiSeq PE300) ...
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bioRxiv - Genetics 2020Quote: ... 400ng of total RNA (RIN >6) was used to generate polyA-selected libraries with Kapa mRNA HyperPrep kits (Roche) with indexed adaptors ...
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bioRxiv - Molecular Biology 2020Quote: ... The digested RNA samples were then analyzed by electrophoresis (5.5% PAGE with 8 M urea) and northern blotting using the DIG Northern Starter Kit (Roche). Poly(A ...
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bioRxiv - Cancer Biology 2019Quote: ... Libraries were pooled at equimolar concentrations then the pool was quantitated using the KAPA library quantification kit (KAPA Biosystems). Libraries were sequenced single end 75 base pairs using NextSeq500 (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... the libraries were prepared from 500 pg of immunoprecipitated DNA fragments using the KAPA Hyper Prep Kit (KAPA Biosystems). The libraries were applied to Single-end sequencing for 93 cycles on HiSeq2500 (Illumina) ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from swab samples or homogenized tissues using a High Pure Viral RNA Kit (Roche, Germany). Reverse transcription and TaqMan quantitative PCR were performed using a probe one-step real-time quantitative PCR kit (TOYOBO ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of each RNA sample was used for library construction using the KAPA mRNA HyperPrep Kit (KAPA Biosystems). Eighteen libraries differentially indexed using the FastGene Adaptor kit (Nippon Genetics ...
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bioRxiv - Immunology 2020Quote: ... The library was constructed following the manufacturer protocol of the KAPA LTP Library Preparation Kit (KAPA Biosystems, Roche, Switzerland). ChIP DNA libraries were ligated with the Bioo scientific barcoded adaptors (BIOO Scientific ...
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bioRxiv - Immunology 2020Quote: ... The library was constructed following the manufacturer protocol of the KAPA LTP Library Preparation Kit (KAPA Biosystems, Roche, Switzerland). ChIP DNA libraries were ligated with the Bioo scientific barcoded adaptors (BIOO Scientific ...
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bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared from high-quality RNA samples (RIN scores >9.0) with KAPA Stranded RNA-seq Kit with RiboErase (Roche). Paired 150-bp reads (21.2 - 118.8 million reads per sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Detection of the respective bands was carried out by the Dig DNA labeling and detection kit (Roche, Penzberg, Germany) according to the manufacturers instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... Antisense Digoxygenin-UTP riboprobes were synthesized using SP6 or T7 RNA polymerases and the DIG RNA Labeling kit (Roche).
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bioRxiv - Cell Biology 2021Quote: TUNEL assay [47] was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq libraries were prepared from 100 ng of total RNA using RNA HyerPrep kit with RiboErase (Kapa Biosystems). Sequencing was performed at the MD Anderson core facility equipped with an Illumina HiSeq 4000 instrument ...
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bioRxiv - Genomics 2021Quote: ... We performed capture-based target enrichment using the SeqCap EZ Hybridization and Wash Kit (Roche NimbleGen, Madison, WI, U.S.). We then used the sequencing platform ...
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bioRxiv - Genomics 2021Quote: ... Pre-capture libraries were prepared from up to 100 ng input genomic DNA using the Kapa Hyperplus kit (Roche), using a fragmentation time of 8 minutes and standard-chemistry end repair/A-tailing ...
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bioRxiv - Genomics 2020Quote: ... The fragmented DNA was then ligated with dual-indices using a KAPA Hyper prep PCR-free library kit (Roche) according to the manufacturer’s directions ...
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bioRxiv - Zoology 2021Quote: ... nasal and tonsil swab) to a final elution volume of 80ul using MagNA Pure LC RNA isolation kit (Roche) and the KingsFisher Flex 96 robot (Thermofisher) ...
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bioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing libraries were loaded on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was carried out with 500 ng sheared DNA using Kapa Hyper library prep kit (Roche, Basel, Switzerland). Barcodes were added using the Illumina UD 96 index kit (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... The isotypes of anti-Drosophila antibodies were determined by the IsoStrip™ Antibody Isotyping Kit (11493027001 Roche, Basel, Switzerland) according to the instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of total RNA was reverse transcribed using a Transcriptor First Strand cDNA synthesis kit (Roche Applied Science) and the provided random hexamer primers for the mix preparation ...
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bioRxiv - Genomics 2020Quote: ... An Illumina compatible NGS library was then prepared with 50ng of genomic DNA using the KAPA HyperPlus kit (Roche). Sequencing was performed on a NextSeq500 instrument (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... Stranded RNA libraries were prepared with 100ng total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche). RNA sequencing was performed on Illumina NovaSeq 6000 system (Illumina) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from BHI broth cultures using the High Pure PCR template preparation kit (Roche, Potsdam, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... The library concentration was determined by qPCR using the Library Quantification Kit Illumina Platforms (Kapa Biosystems, Boston, MA, USA). An equimolar pool of the barcoded libraries was made and the pool was sequencing on a HiSeq 2500 system (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... stranded RNA libraries were prepared with 100ng total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche). RNA sequencing was performed on Illumina NovaSeq 6000 system (Illumina).
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bioRxiv - Genomics 2020Quote: ... the total DNA was sheared to ~500 bp size fragments and processed using KAPA LTP Library Preparation Kit (Roche Sequencing Solutions ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared from these diluted samples using a miniaturized version of the KAPA HyperPlus kit (Roche, KK8514) with Illumina-compatible KAPA Unique Dual-Indexed Adapters (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... antisense and sense digoxogenin (DIG)-labeled RNA probes were generated using the DIG RNA labeling kit (Roche Diagnostics, Germany), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using the Transcriptor First-Strand cDNA Synthesis Kit with anchored oligo (dT)18 primers (Roche; 04379012001). Gene expression levels were analyzed by real-time PCR on an Applied Biosystems model 7500 Software (version 2.0.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... The TUNEL method was used to quantify apoptosis following the manufacturer’s instructions (In Situ Cell Death Detection kit; Roche). The percentage of TUNEL-positive cells in each kidney was quantified as described above ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed following citrate buffer antigen retrieval using an in situ cell death detection kit (Roche, 11684795910). Positive control slide was treated with deoxyribonuclease to induce DNA damage (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, KK4610). cDNA was synthesized from human cells using SuperPrep II Cell Lysis & RT Kit (TOYOBO ...
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bioRxiv - Immunology 2022Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
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bioRxiv - Genomics 2022Quote: A DNA library was prepared from the WGA sample using the Kapa LTP Library Preparation Kit (KAPA Biosystems, Roche7961880001) and sequenced on the Illumina MiSeq platform with the MiSeq Reagent Kit v3 to generate 2×150 bp paired-end reads ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared by the Bauer Core Facility at Harvard University using a KAPA stranded RNA HyperPrep Kit (Roche Sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction were carried out using MagNA Pure LC total Nucleic acid isolation kit on the automated MagNA Pure LC2.0 platform (Roche) from two to five colonies picked up from fresh cultured plates.
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bioRxiv - Microbiology 2022Quote: ... Library preparation was performed using ∼ 1ug of total RNA processed using RiboErase kits to remove ribosomal RNA (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 °C for 8 min ...
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bioRxiv - Microbiology 2022Quote: Quantitative Real-time PCR assays were performed with the KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) on a LightCycler 1.5 (Roche ...
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bioRxiv - Microbiology 2022Quote: ... the water phase was used for RNA isolation with High Pure RNA Isolation Kit (Roche Diagnostics GmbH, Mannheim, Germany), yielding >3 μg total RNA per sample ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction was carried out using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche, Diagnostics) as per the manufacturer’s instructions ...