Labshake search
Citations for Roche :
4101 - 4150 of 5370 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
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bioRxiv - Plant Biology 2024Quote: ... and the library was quantified by qPCR against a standard curve with the KAPA Library Quantification Kit (Kapa Biosystems). Libraries were sequenced on a NovaSeq6000 Illumina platform in 150PE mode ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of the PCR product was used for RNA probe labeling with T7 RNA polymerase as per the DIG Northern Starter Kit’s Instruction Manual (Roche). Post-hybridization washes and immuno-chemiluminescent detection of the bound probe were conducted following the instructions provided in the DIG Northern Starter Kit manual (Roche) ...
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bioRxiv - Plant Biology 2024Quote: We fragmented 700 ng of high-molecular-weight DNA using a Covaris S220 sonicator and a WGS library was generated for both species using the KAPA Hyper Prep kit with a PCR-free protocol according to the manufacturer’s instructions (Roche). We applied final size selection using a 0.7-fold ratio of AMPureXP beads ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 1 µg of total RNA using the KAPA Stranded mRNA Hyperprep Kit (96rxn) (Roche, KK8581) according to manufacturer’s specifications ...
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries were prepared using 100 ng fragmented DNA with the KAPA HyperPrep Library Prep Kit (Roche, Indianapolis, IN) and NEXTFLEX UDI barcodes (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...
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bioRxiv - Molecular Biology 2024Quote: ... membrane hybridization and detection were performed using DIG-High Prime DNA Labelling and Detection Starter Kit II (Roche, #11585614910) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Molecular Biology 2024Quote: Analysis of ChIP-purified DNA by quantitative PCR (qPCR) was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and StepOnePlus real-time PCR systems (Life Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche, #11175025910). Probes were then cleaned with MegaClear Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... For library preparation the Kapa LTP Library Preparation Kit for Illumina Platforms was used (Kapa Biosystems, Part Number KK8232). The cDNA was filtered into the End Repair step of the method and the library preparation was followed as per the protocol through to Adapter Ligation ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA prepared using the KAPA Express reagent (a component of the KAPA Mouse Genotyping Kit from Roche, 07961804001) and analyzed for both CRISPR lesions and repeat size as described below.
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bioRxiv - Genetics 2024Quote: ... 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche) with random primer ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated from cells and reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Gene expression was quantified using FastStart Roche SybrGreen (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was performed in two technical replicates using KAPA Hifi Hotstart PCR kit (Kapa Biosystems, MA, USA) via a Bio-Rad CFX96 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We prepared RNA libraries using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adaptors (KAPA Biosystems; Massachusetts, USA), with 200 ng of RNA as input ...
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bioRxiv - Immunology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: The library of mtDNA was prepared from 500 ng mitochondrial DNA with a KAPA HyperPlus Kit PRC-free (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... two PCR reactions were performed with PCR anchor primer (included in the 2nd Generation 5’/3’ RACE Kit, Roche) and GB3 (oligo 61 ...
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bioRxiv - Genomics 2023Quote: TP pre-capture libraries were prepared as previously described [15] using the KAPA HyperPlus kit with UDI adapters (Roche). 17.5µL DNA was used as input for fragmentation for eight minutes ...
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bioRxiv - Microbiology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
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bioRxiv - Immunology 2023Quote: ... Poly(A)-enriched next-generation sequencing library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) with 100 ng of input total RNA and 15 amplification cycles according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 6ng of ChIP DNA was processed for library preparation for Next Generation Sequencing by using Kapa kit (Roche) following the manufacturer’s instruction with one modification ...
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bioRxiv - Genetics 2023Quote: ... The molar concentration of the pool was measured using the KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) and the pool was sequenced on one lane of the Illumina HiSeq 4000 sequencer with a 75 bp Paired-End SBS chemistry (Illumina).
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bioRxiv - Genomics 2023Quote: ... Library concentration was assessed using both Qubit fluorometric quantification and the qPCR-based KAPA Library Quantification Kit (Roche, KK4824). Appropriate fragment size distributions were confirmed by running each sample on an Agilent Bioanalyzer using the DNA High-Sensitivity Kit (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... was introduced by site directed mutagenesis on C-terminal SNAP-tagged H3.1 (HIST1H3C coding sequence cloned in pSNAPm) and H3.3 (H3F3B coding sequence cloned in pSNAPm) encoding plasmids with a PCR master kit (Roche) and the primers indicated in Supplementary table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Highly sensitive DNAprobes labelled with digoxigenin-dUTP were generated using the PCR digoxigenin probe synthesis kit (Roche Applied Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... Real time PCR was performed on the cDNA using KAPA SYBR FAST qPCR Master Mix (2X) kit (Roche, #KK4618) with intron spanning primers in a QuantStudio 3 Real-time PCR system ...
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (cat#: KK4824, KAPA Biosystems, Boston), using 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR ...
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bioRxiv - Neuroscience 2023Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). Sections were treated with Discovery CC1 cell conditioning solution at 95°C for 60 min followed by a low concentration of protease (Protease 3 ...
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bioRxiv - Genomics 2023Quote: ... Quantitative PCR was carried out on standards and test samples using the LightCycler 480 Probes Master kit (Roche, 04707494001) with inverted terminal repeat probe and primer sequences indicated in Table S4.
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bioRxiv - Genomics 2023Quote: ... Library construction was performed on the 5 ng of pooled PCR amplicons using the KAPA HyperPrep Kit (Roche 07962363001) and NEXTFLEX Unique Dual Index Barcodes (7.5µM final concentration ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the molarity of the library was measured using quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed from 10 ng purified DNA using the High Throughput Library Prep Kit from KAPA Biosystems (KK8234) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Sense and anti-sense riboprobes were generated using a digoxygenin RNA labelling kit and T3/T7 RNA polymerases (Roche). Images are representative of at least 3 embryos per condition.
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bioRxiv - Bioengineering 2023Quote: ... and RNA-Seq libraries were prepared using 120 ng of total RNA and Kapa mRNA HyperPrep Kit (Kapa Biosystems). Each library was indexed using NEXTflex DNA Barcodes-24 (BIOO Scientific ...
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bioRxiv - Genomics 2023Quote: DNA extraction for sequencing with Illumina was done using High Pure PCR Template Preparation Kit from Roche (Mannheim, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... all tissue samples were processed using the KAPA Stranded RNA-Seq kit with RiboErase (HMR) (KAPA, Roche, cat. # 08098140702). Libraries were constructed according to the manufacturer protocol and were then quantified using an Agilent 2100 Tapestation ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems, KK4601). The expression levels of the target genes were normalized against that of tomato ACTIN2 ...
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bioRxiv - Physiology 2023Quote: ... Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche, Cat. 12156792910). Frozen pancreata for histology were removed and fixed as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from the FFPE tissue by use of the High Pure FFPE RNA Micro Kit (Roche 04823125001). Slides were cut into 2 10 micron sections ...