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Citations for Roche :
3951 - 4000 of 5370 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted by combining phenol-chloroform–isopropanol treatment using the High Pure RNA Isolation Kit (Roche, Germany) (101) ...
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bioRxiv - Genomics 2020Quote: ... The sheared DNA was used for paired-end library preparation with a KAPA LTP Library Preparation Kit (KAPA Biosystems). The optimal numbers of PCR cycles for individual libraries were determined with a Real-Time Library Amplification Kit (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems; Cat#KK4835). Libraries were then pooled and sequenced (1×75bp ...
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bioRxiv - Genomics 2019Quote: We constructed dual indexed libraries using 100-250ng of genomic DNA and the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: One microgram of total RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with the oligonucleotide (dT)18 primer according to the manufacturer’s instructions (30,34) ...
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bioRxiv - Physiology 2019Quote: ... Free fatty acid and triglyceride levels were measured in serum using the colorimetric quantification kits Half-micro test (Roche) and Infinity (ThermoScientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed by the Dana-Farber Molecular Biology Core Facility using the KAPA mRNA HyperPrep Kit (Roche). Nucleic acid was sequenced using an Illumina NextSeq 500 instrument (PE75 kit).
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bioRxiv - Microbiology 2019Quote: ... and qPCR based library quantification was performed using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems, KK4824) on a LightCycler480 (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: ... To generate the dsRNAs for the rescreen cDNA was generated using the Transcriptor First Strand cDNA Synthesis Kit (Roche). The cDNA was then used to amplify fragments by PCR with the same primers as used by Eupheria Biotec GmbH (Dresden ...
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bioRxiv - Genetics 2020Quote: ... Staining for apoptotic cells was performed using the AP-In situ Cell Death Detection Kit (Roche Diagnostics, Penzberg, Germany) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... The digoxigenin-labeled RNA probes were prepared using a DIG RNA labeling kit according to the manufacturer’s protocol (Roche) using each cDNA clone as the template ...
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bioRxiv - Neuroscience 2019Quote: ... Antisense and sense digoxigenin (DIG)-labelled RNA probes were generated using the DIG RNA labelling kit (Roche Diagnostics, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 40 ng of DNA per sample was subjected to qPCR analysis using LightCycler 480 Probe Master-Mix kit (Roche) and primer sets for γHV68 gB and host NFAT5 (Table S3) ...
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bioRxiv - Genomics 2019Quote: K111 insertions were amplified by PCR using the Expand Long Range dNTPack PCR kit (Roche Applied Science, Indianapolis, IN) as described ...
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bioRxiv - Neuroscience 2019Quote: ... barcoded and enriched using the NimbleGen SeqCap EZ Human Exome Library v2.0 enrichment kit (Roche NimbleGen, Madison, WI, USA). Purified and quantified library pool was subsequently sequenced on an Illumina HiSeq 2000 sequencing instrument (Illumina ...
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bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR was performed with the LIght Cycler DNA Fast Start Master SYBR green I kit (Roche) in a Light Cycler 480 Instrument II at Tm=60°C ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-sequencing libraries were then prepared either manually with the KAPA Stranded mRNA-seq library prep kit (KAPA Biosystems), or with automation ...
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bioRxiv - Genomics 2019Quote: ... Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal) (Kapa Biosystems, Woburn, MA). The libraries were pooled and sequenced on a 100 bp paired-end Illumina HiSeq 2500 or 4000 run (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Total RNA sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase for Illumina Platforms (KAPA Biosystems). Briefly ...
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bioRxiv - Genomics 2021Quote: ... RNA-sequencing (RNA-seq) libraries were generated from 300ng RNA using the KAPA RNA HyperPrep Kit with RiboErase (Roche) and sequenced with NovaSeq 6000 (2×150 bp ...
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bioRxiv - Cell Biology 2021Quote: ... A cDNA library was made from Oligo(dT)12–18 as described in the manufacturer’s protocol (Transcriptor First Strand cDNA Synthesis Kit, Roche). Quantitative PCR was performed using Quantifast SYBR Green PCR Kit (204052 ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using 50 ng of DNA with the KAPA HyperPlus Kit as per the manufacturer’s protocol (Roche). The libraries were sequenced with a paired-end read length of 150bp ...
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bioRxiv - Microbiology 2021Quote: ... The final pool was quantified by qPCR with Kapa Library Quant Kit for Illumina Platforms (KapaBiosystems, Roche, Cat. # KK4824) on an AriaMX Real Time PCR System (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from clinical isolates using the AMPLICOR® Respiratory Specimen Preparation Kit (Roche, Basel, Switzerland) and purified with 1.8X AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Samples were further processed using the Kapa LTP Library Preparation Kit for Illumina Platforms (Kapa Biosystems, Part Number KK8232) and indexed with the NEBNext Multiplex Oligos for Illumina 96 Unique Dual Index Primer Pairs (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... Prepared libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit (Kapa Biosystems / Roche AG, Basel, Switzerland) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Bioengineering 2020Quote: ... Prepared libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit (Kapa Biosystems / Roche AG, Basel, Switzerland) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Neuroscience 2021Quote: ... The eaat2a target site was PCR amplified with a fast-cycling polymerase (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) (primers ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 400 ng (melanoblasts) or 1µg (cell lines and tissues) input RNA using Transcriptor first-strand cDNA synthesis kit (04897030001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was performed with equal RNA input using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Biochemicals). Quantitative PCR was performed using C1000 Touch Thermal Cycler (BioRed ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from LOUCY cells using the High Pure RNA Isolation Kit (Roche Life Sciences, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... NPCs were washed by phosphate buffer saline (PBS) and stained by in situ Cell Death Detection Kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The complementary DNA (cDNA) was synthesized using a transcriptor high fidelity cDNA synthesis kit (Roche Applied Science, Penzberg, Germany). Each prepared sample used 20 µL of the SYBR green qPCR reaction kit (Roche Applied Science) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... pooled and quantified by qPCR with the KAPA Library quantification kit for Illumina platforms (Kapa Biosystems, Wilmington MA, USA) on a StepOnePlus qPCR system (Thermo-Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the culture supernatant after each passage using a High Pure Viral RNA kit (Roche Diagnostics). For deep sequencing of the S1/S2 cleavage site of the viral S gene ...
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bioRxiv - Neuroscience 2021Quote: ... The library amplification step included 6-7 PCR cycles and was performed using the KAPA Library Amplification kit (Roche). The final Hi-C library was purified using SPRIselect beads and the quality and size of the library (approximately 500 bp ...
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bioRxiv - Genetics 2021Quote: BAM files from previously published multi-region WES data (generated using the SeqCapEx Exome Enrichment Kit v3.0 (Nimblegen/Roche)) from nine sporadic fresh-frozen adenomas were available (Supplementary Table 2).11 Somatic variants were re-called using Mutect2 as above.
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bioRxiv - Microbiology 2021Quote: ... The 16S rRNA V3–V4 amplicon was amplified using a KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, USA). the amplicon PCR forward primer (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... 4 were purified according to the instructions of the manufacturer using the High Pure PCR Template Preparation Kit (Roche) followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
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bioRxiv - Plant Biology 2021Quote: ... Library preparation and sequencing were performed by the iGenSeq genotyping and sequencing core facility (ICM Institute - Hôpital Pitié-Salpêtrière AP-HP, Paris, France) using KAPA HyperPrep Kits (Roche, Basel, Switzerland) and the NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The qPCR was performed using KAPA SYBR® FAST qPCR Kit Master Mix (2X) Universal (Kapa Biosystems; Massachusetts, USA). Protein expression at day 10 post transfection was analyzed by immunocytochemistry using the following antibodies and dilutions ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL staining was performed according to respective manufacturer protocol using Terminal Deoxynucleotidyl Transferase kits (Invitrogen #10533065 or Roche #03333574001), dig-UTP (Roche ...
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bioRxiv - Genomics 2020Quote: A screenshot showing read visualization by IGV of the region were the Alu insertion occurred in the positive control sample (BRCA1) when using the SeqCap EZ Exome v3.0 kit from Roche for exome capture is presented in Figure 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100-500 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Biophysics 2021Quote: ... The troponin T detection kit (Elecsys® Troponin T Gen 5 STAT) and troponin T were purchased from Roche. 1X phosphate-buffered saline (PBS ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: RNA was converted into cDNA and made into sequence ready libraries with the KAPA RNA-Seq Kit (Kapa Biosystems). RNA-Seq libraries were sequenced with 150 bp single-end reads on a HiSeq 2500 ...
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bioRxiv - Cell Biology 2020Quote: RACE-cDNA was synthesized according to the manufacturer’s protocol for 5’/3’ RACE Kit 2nd generation (Roche; Cat.No. 03353621001). The 2648-bp cDNA was cloned into the NheI-XhoI site in pcDNA 3.1 plasmid to obtain pcDNA3.1-DR5-AS and was verified by sequencing ...