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Citations for Roche :
4051 - 4100 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Developmental Biology 2023Quote: ... we dissected 10 testes from one-day-old adults or 10 imaginal wing discs from 3rd instar larvae in PBS buffer supplemented with cOmplete protease inhibitor (Roche 11697498001). Dissected tissues were permeabilized with 0.1% Triton/PBS for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Immunology 2023Quote: ... 1% NP-40, 150 mM NaCl, and 10 mM Tris-HCl [pH 8] supplemented with phosphatase and protease inhibitor cocktail tablets [Roche Diagnostics]). The cell lysates were cleared by centrifugation for 15 min at 40C ...
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bioRxiv - Microbiology 2023Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Immunology 2023Quote: ... The upper aqueous phase was collected and mixed with 700 µl chloroform (Roth, 7331) and glycogen (Roche, 10 901 393 001). The RNA was then precipitated at -20°C for 16 h and pelleted by centrifugation at 4600×g for 33 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in lysis buffer (100 mM HEPES pH 7.5, 200 mM NaCl, 10% glycerol) supplemented with EDTA-free Protease inhibitor cocktail (Roche, Cat#05056489001), 1 mM phenylmethylsulfonylfluoride (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, cOmpleteTM Mini Protease Inhibitor (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µL of cold buffer D (50 mM Tris/HCl, pH = 8.0, 10 mM EDTA, 1% SDS 1xPIC (cOmplete™, Protease Inhibitor Cocktail; Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µl cold buffer D (50 mM Tris/HCl (pH=8.0), 10 mM EDTA, 1% SDS, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Molecular Biology 2023Quote: ... extract was prepared using Co-IP buffer (50 mM Tris/Cl (pH=7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001), 0.5 mM PMSF) ...
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bioRxiv - Immunology 2023Quote: ... coated with 2.5ug/mL aCD3 and re-stimulated for an additional 3 days in complete IMDM-10 supplemented with 50U/mL IL-2 (Roche 11011456001). For Th17 polarizations ...
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bioRxiv - Immunology 2023Quote: ... then subject to gentle cold digestion on ice using 10% FBS-supplemented RPMI media containing 100mg/mL trypsin inhibitor from soybean (Roche, 10109886001), 10mg/mL collagenase A from clostridium histolyticum (Roche ...
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bioRxiv - Physiology 2023Quote: ... The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler ...
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bioRxiv - Cell Biology 2023Quote: ... Crypts were isolated from the distal half of the small intestine as described above and dissociated in 10% FBS in 1X PBS supplemented with 20 μg/mL Liberase TH (Roche 05401135001), and 35 μg/ml DNaseI (Roche 10104159001 ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor). The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... mycelia were resuspended in lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg/mL lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany)) and incubated at 37°C for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in lysis buffer (50 mM NaH2PO4; 300 mM NaCl; 10 mM imidazole; pH 8: supplemented with protease inhibitor cocktail (cOmplete, Roche, Switzerland), DNaseI (ITW REAGENTS ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 20 ml of S100 Lysis Buffer (10 mM KHPO4 pH 7.5, 1x Mini protease inhibitor cocktail tablet (Roche, Basel, Switzerland)) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Infected red blood cells were lysed 10 min on ice with 0.1% saponin in PBS complemented with 1x Protease Inhibitors Cocktail (Roche; PBS+PI). Parasites were pelleted and washed with PBS+PI to remove haemoglobin ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8) supplemented with Proteinase Inhibitor Cocktail (Roche, 11697498001) and chromatin was sonicated using the Bioruptor pico (Diagenode ...
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM NaCl, 2 mM MgCl2, 10 mM NaF, 1 mM PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 45 min on ice and centrifuged at 20000g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Cryosections were then washed 3 times for 5 minutes in PBT before being incubated with 1:10 TUNEL enzyme buffer (Roche #12156792910) at 37°C in the dark for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... This was done by using cDNA from 10 ng total RNA with 2x FastStart Universal Probe Master ROX (Roche, Basel, CHE) and the corresponding TaqMan Assay in a StepOnePlus Real-Time PCR Cycler (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM imidazole) supplemented with DNase and one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche #11 873 580 001). Cells were lysed using a French press (2x ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Cells were grown to ∼80% confluency, washed in PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, and protease inhibitors (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: Probes were prepared by PCR amplification using a PCR-DIG labelling kit (Roche) as directed by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... We made Lhk and CK2ßtes-Y probes using PCR Nick Translation kits (Roche) and ordered oligo probes from IDT ...
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bioRxiv - Cancer Biology 2021Quote: ... Q-PCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche). PCR amplification was performed as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared using KAPA mRNA (PolyA) HyperPrep Kit (Roche Holding AG, KK8581) using 500 ng RNA per sample according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... DNA library preparation was performed with the Kapa HyperPrep kit (Roche, Basel, Switzerland), followed by exome target capturing with the SeqCap EZ exome probes V3 kit (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using the Transcriptor first strand cDNA synthesis kit (Roche, 04379012001). Gene expression levels were analyzed by real-time PCR on an Applied Biosystems model 7500 (software v2.0.5 ...
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bioRxiv - Immunology 2021Quote: ... and the final library was quantified using the KAPA Library Quantification Kit (Roche). Pools were sequenced on an Illumina HiSeq 2500 machine using the rapid run setting with single end reads.
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using a High Pure PCR Product Purification Kit (Roche). Customized oligonucleotide primers (S1 Table ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA samples were isolated with an RNA isolation kit (Roche Product No: 11828665001). Purity and concentration measurements were taken with nanodrop at 260-280 nanometers ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was conducted using a SYBR Green Master qPCR Kit (Roche Diagnostics, USA). The relative expression levels were calculated by the ΔΔCT method (Schmittgen & Livak ...
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bioRxiv - Cell Biology 2020Quote: ... Messenger RNAs were reverse-transcribed with Transcriptor First Strand cDNA Synthesis Kit (Roche). qPCR were performed using the FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... QPCR was performed using LightCycler 480 SYBR Green I Master kit (Roche, Switzerland) on LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2019Quote: ... reverse transcription was performed using the Transcriptor first strand cDNA synthesis kit (Roche) and cDNA analysed on custom real-time ready 384 well custom PCR arrays (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified using the High Pure PCR product purification kit (Roche). Libraries were quantified with Qubit high sensitivity assays ...
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bioRxiv - Developmental Biology 2021Quote: ... They were quantified with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturer’s library quantification protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... eurysternus GA72 sample using the Hyper Library construction kit from Kapa Biosystems (Roche). The library was sequenced in a multiplexed mode on one SP lane of NovaSeq 6000 for 251 cycles from both ends of the fragments ...
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bioRxiv - Developmental Biology 2021Quote: ... Barcoded sequencing libraries were generated using a KAPA HyperPrep Kit (Roche, No. 07137923001) following the Nimblegen SeqCap EZ Library protocol (Version 4.3 ...
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bioRxiv - Genomics 2020Quote: ... The library was prepared with KAPA Stranded RNA-seq Kit with RiboErase (Roche). RNA-seq reads (120 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was then amplified with the SYBR Green Master kit (Roche, Mannheim, Germany) using a Light Cycler 480 apparatus ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were purified with the High Pure PCR product purification kit (Roche) and digested with SacI and XbaI enzymes in M buffer (TaKaRa) ...
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bioRxiv - Genetics 2022Quote: ... and constructed Illumina libraries using KAPA LTP Library Preparation Kits (Roche, Basel, Switzerland). We sequenced both libraries on an Illumina HiSeq4000 at Novogene (Beijing ...