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Citations for Roche :
3951 - 4000 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml vPBS (PBS, 46 mM sucrose, 10 mM glucose) containing EDTA-free protease inhibitors (Roche). The washed cells were pelleted by centrifugation (750xg ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 mL dissociation buffer (RPMI-1640 (Cytiva) with 25 mM HEPES and 10 µg/mL gentamicin sulfate) supplemented with 2 mg/mL Collagenase-D (Roche) and 100 U DNaseI (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear pellets were resuspended in shearing buffer (0.1% SDS, 1 mM EDTA, 10 mM Tris-HCl pH 7.5, 1X Roche protease inhibitor, and 1X Roche phosphatase inhibitor) and transferred into 130 μl Covaris tubes (Covaris ...
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bioRxiv - Biochemistry 2023Quote: ... An on-column DNA digestion was performed to remove the residual genomic DNA in total RNA using DNase I (10 U/μL, Roche) and RDD buffer (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged for 10 min at 16000 xg and resuspended in 300 µl PBS containing protease inhibitors (cOmplete, Roche). A 16 µl sample was added to 5 µl of 5x SDS sample buffer (300mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 7000 xg for 15 minutes and were lysed in 10 ml PBS supplemented with protease inhibitor (cOmplete, Mini, EDTA-free, Roche). NatT was purified by immunoprecipitation using Anti-FLAG magnetic beads (Merk ...
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bioRxiv - Plant Biology 2024Quote: ... 100 mM NaCl, 80 mM KCl, 1% glycerol, 0.1 % Triton, 10 mM DTT, plus 1 mini protease inhibitor cocktail tablet, Roche, Switzerland) per 20 ml of extraction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... uteri were harvested from pregnant mice at 4.5 days post coitus and washed with cold swelling buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 1X Protease Inhibitor Cocktail (PIC, Roche, 11836170001)) immediately after collection ...
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bioRxiv - Cancer Biology 2024Quote: ... in buffer (10 mM Tris-Cl pH8.0, .25 mM EDTA, 100mM NaCl, 0.5% SDS 1mg/ml proteinase K) (Roche Cat #03508838103) at 55 °C for 4 hours to remove proteins ...
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bioRxiv - Neuroscience 2024Quote: ... The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche) in the presence of primers at 500 nM ...
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bioRxiv - Biophysics 2024Quote: ... 2.0 × 106 cells/mL of WT Neuro2a or OptoTau KI cells were incubated in 10 mM of HEPES buffer supplemented with 1% protease inhibitor (cOmplete, Roche), 1 µM of okadaic acid (Cayman Chemical) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed 3x 15 minutes in room temperature TBST (Tris-buffered saline with 1% Tween 20) and blocked at room temperature for one hour in 10% DIG buffer (Roche) in TBST.
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bioRxiv - Genomics 2024Quote: ... in nuclear lysis buffer (50 mM Tris-HCl [pH 7.5], 10 mM NaCl, 0.2% NP-40, 1x Roche protease inhibitors) at 4
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bioRxiv - Molecular Biology 2024Quote: ... samples were washed three times with 10 ml of ice-cold PBS supplemented with Complete Protease Inhibitor (Roche, Basel, Switzerland), and the cells were resuspended in 1 ml of the same buffer ...
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bioRxiv - Genomics 2024Quote: ... Nuclei were isolated by carefully transferring the extracted parasite mixture on top of a 0.25 M to 0.1 M sucrose gradient in cell lysis buffer (10 mM Tris pH 8, 3 mM MgCl2, 0.2% NP-40, 1x EDTA free Protease inhibitor (Roche, 04693132001); 15 mL 0.25 M Sucrose and 17.5 mL 0.1M Sucrose for 50 mL tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Pathology 2024Quote: Volumes of EV lysates containing approximately 110 µg of proteins were treated with 2.5 μl of DNAse I recombinant (10 Units/µl) (Roche Diagnostics), 5 μl of 10X DNase buffer ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by discarding 100 ul from each well and adding 100 ul of solution containing DMEM with 10% FBS and WST-1 reagent (Roche Diagnostics) diluted 1:10 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
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bioRxiv - Cell Biology 2020Quote: ... cells were collected via centrifugation and resuspended in lysis buffer (40mM Tris pH 8, 300mM NaCl, 10% glycerol, 10mM imidazole, 1mM DTT, 1mM PMSF and 1x Roche PIC). Cells were lysed by sonication and after clearing the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR reactions were conducted in a 10-µL final volume with 5 µL of 2X FastStar Universal SyBR green Master (Roche, Switzerland), 1.5 µL of forward/reverse primer mix (Table S1 ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.1 mM EDTA) supplemented with 10 mM 2-mercaptoethanol (BME) and four EDTA-free cOmplete protease inhibitor tablets (Roche Applied Science) prior to lysis using the Nano DeBEE High Pressure Homogenizer (BEE International) ...
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bioRxiv - Physiology 2022Quote: ... 2 mmol/l MgCl2, 10 mmol/l NaF, 1 mmol/l PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 30 min on ice and centrifuged at 20,000 g for 10 min at 4ºC ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was stopped by the addition of 500 ng of plasmid (size > 10 kb) and 0.1 pmol of ATP-γ-S tetralithium salt (10102342001-Roche, Sigma) followed by incubation for 30 min at 30°C ...
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bioRxiv - Immunology 2021Quote: ... membranes were washed three times in TBS-T followed by blocking in 10% (v/v) western blotting reagent (Roche, Basel, Switzerland) for 1–2 h at room temperature ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... 50 μg of cell extract from each group was separated on 10% Tris-HCl gel and then electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Roche, UK) o/n at +4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell pellet were thawed in 500 μl hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %, DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease) and incubated at room temperature for 15 mins (for nuclease activity) ...
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bioRxiv - Developmental Biology 2019Quote: Cell pellet were thawed in 300 μl hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %, DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease) and incubated at room temperature for 15 mins nucleic acid digestion ...
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bioRxiv - Cancer Biology 2019Quote: ... The nuclear pellet was brought up to 880 μL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1× Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Immunology 2019Quote: ... and each of the three nested PCR steps was carried out in a total volume of 10 µL using 1 µL cDNA/PCR template and KAPA HiFi HotStart ReadyMix (Kapa Biosystems). In the last PCR reaction ...
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bioRxiv - Genomics 2019Quote: ... The pellet was resuspended in sonication buffer (1% SDS, 50mM Tris pH8, 10 mM EDTA, 1x protease inhibitor (cOmplete™, Mini, EDTA-free, Roche)) and sheared for 25 minutes (30sec ON ...
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bioRxiv - Genomics 2020Quote: ... The oligonucleotide pool was PCR-amplified in 10 cycles (Primers stated in Supplemental Information) and KAPA® HiFi HotStart Polymerase (Roche) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The nuclear pellet was brought up to 880 µL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1x Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Genetics 2019Quote: ... which was diluted 1:10 to be used in RT-qPCR carried out using LightCycler 480 SYBR Green I Master (Roche, 4707516001) under the following conditions ...
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bioRxiv - Neuroscience 2019Quote: ... pH 8.0 and 10% sucrose) along with 1X PhosSTOP phosphatase and 1X cOmplete protease buffer (Roche Applied Science, Indianapolis, IN, USA). The homogenized samples were centrifuged at 4°C for 15 min at 18,000g ...
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bioRxiv - Neuroscience 2020Quote: ... the cDNA of our samples were diluted 10 fold and 4 µl were added to a mix of 5 µl FastStart Universal SYBR Green Master (Roche, Germany), 0.4 µl of nuclease-free water and 0.3 µl of forward and reverse primer (see primer sequences in Supplementary Table 1) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were homogenized in 2 mL co-IP lysis buffer (20 mM Tris-HCl, pH = 7.6; 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Nonidet P40 Substitute; protease inhibitor cocktail [Roche]; PhosSTOP [Roche]), using a Dounce homogenizer ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant MYC was eluted from the beads using SUMO elution buffer (250 mM imidazole, 10% glycerol, 1X PBS, 2 mM DTT) + PIC (Roche 05056489001).
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bioRxiv - Cancer Biology 2020Quote: ... 20 mM imidazole), and 1X with SUMO wash buffer (3 mM imidazole, 10% glycerol, 1X PBS, 2 mM DTT) + PIC (Roche 05056489001). Recombinant MYC was eluted from the beads using SUMO elution buffer (250 mM imidazole ...
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bioRxiv - Cancer Biology 2020Quote: ... washed twice in PBS and resuspended in 100 μL annexin V incubation buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 5 mM CaCl2) containing 1% annexin V FLOUS (Roche Molecular Biochemicals) and 500 μg/μl PI stain ...
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bioRxiv - Cell Biology 2021Quote: ... lysed by sonication in ice-cold Tris buffer (50 mM Tris pH 8.0, 150 mM NaCl, 10 mM imidazole, 2 mM βME, complete protease inhibitors (Roche, Basel, Switzerland), 1 μg/ml DNase ...
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bioRxiv - Neuroscience 2019Quote: ... containing 0.15M NaCl and 0.05% Tween and then blocked by 10% goat serum and 1% blocking reagent powder (Roche Diagnostics, Mannheim, Germany) in 0.1M Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... 300 mM sucrose, 150 mM NaCl, 10 mM potassium acetate, 5mM EDTA, and a protease inhibitor mixture [Complete EDTA-free; Roche Diagnostics]) using a mortar and pestle ...
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bioRxiv - Microbiology 2020Quote: ... The PCR was performed in 10 μl volume reactions with 0.2 μM primer concentration and using the Kapa HiFi HotStart Ready Mix (Roche, ref. KK2602). Cycling conditions were initial denaturation of 3 min at 95 °C followed by 20 cycles of 95 °C for 30 s ...
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bioRxiv - Microbiology 2020Quote: ... Pellet was resuspended in 40mL lysis buffer (20mM HEPES, pH 7.4, 10% glycerol, 0.2M MgSO4, 1M NaCl, 1mM EDTA, 1mM DTT, Roche protease inhibitor cocktail). Lysozyme was added at a final concentration of 1mg/ml and cells were incubated on ice for 30 minutes ...