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Citations for Roche :
4051 - 4100 of 6260 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-GFP (1:2’000; Roche), anti-HA (clone 16B12 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100] containing a protease inhibitor cocktail (cOmplete ULTRA Tablets, # 05892791001, Roche, Basel, Switzerland) and 0.3 mg/ml phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Plant Biology 2021Quote: ... The transgene expression was maintained by cultivation on media supplemented with 40 μg.ml-1 hygromycin (Roche) and 100 μg.ml-1 cefotaxime (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM of phosphatase inhibitors (β-glycerophosphate, NaF and NEM) and 1 tablet of PhosStop (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... then lysed in cold PBS/1% NP40 including the cOmplete EDTA-free protease inhibitor cocktail (Roche) or virus was released by freeze-thawing and ultrasonic treatment for analysis in viral plaque assay (see above).
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used in this study were: rat anti-HA (1:250; Roche 3F10, RRID: AB_2314622), rabbit anti-cleaved Caspase-3 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT and complete EDTA-free protease inhibitor tablets (one tablet/50 mL buffer) (Roche)) ...
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bioRxiv - Microbiology 2020Quote: ... Amplicon PCRs were conducted in 25 μL reactions each containing 1 X Buffer (KAPA Biosystems, USA), 1.5 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... pH 8) supplemented with protease inhibitors (1 tablet of complete protease inhibitor per 20 mL; Roche, Mannheim ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended in 1 ml staining buffer containing 0.1 mg/ml RNaseA (Roche Life Sciences) and incubated at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP) using X-treme GENE HP (Roche) (for details on cell culture and transfection see Amin et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Sample were resolved via SDS-PAGE and immunoblotted for GFP (Roche AG, Basel, Switzerland, 1:1,000) and Pgk1 (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed twice in PBST then incubated with DAPI (1 μg/ml in PBS; Roche) for 5 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and washed with 1 mL 1X Wash Buffer (1 mL HEPES pH 7.5, 1.5 m 5M NaCl, 12.5 uL 2M spermidine and 47.5 mL dH2O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Neuroscience 2022Quote: ... or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5, 1.5 m 5M NaCl, 12.5 uL 2M spermidine and 47.5 mL dH2O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) with the addition of two EDTA-free cOmplete protease inhibitor cocktail (Roche, 11836170001). Supernatant from lysed bacteria was collected by centrifugation at 16000 g for 10 minutes at 4°C ...
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bioRxiv - Physiology 2022Quote: ... the spleen was additionally digested in HBSS medium containing 1 mg/ml Collagenase D (Roche, 11088882001) and 50 U/ml DNase I for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated overnight at 4 °C with rat anti-HA Abs at 1:200 (Roche) and guinea-pig anti-red fluorescent protein (RFP ...
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue pellet was resuspended in a solution of HBSS with 1 mg/ml collagenase A (Roche) and 1 mg/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: The following primary antibodies were used in this study: anti-HA (rat, 1:200, 11867423001, Roche), anti-V5 (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... Cell membranes were solubilized in the lysis buffer plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Triton X-100 and 10% glycerol in PBS) supplemented with a protease inhibitor cocktail (Roche) and 50 µM Palmostatin B (for SwissKASH-derived samples) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Hybridization signals were detected by Anti-Digoxigenin-FITC (Roche; dilution 1:10 in 0.5% BSA/PBS) and Streptavidin-Cy3 (Invitrogen Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated for 1h in 1% milk prepared in TBST with anti-GFP antibody (Roche). The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... ATP-induced dissociation was tested with an addition of running buffer containing ATP (1 mM, Roche) following binding of the MSH homolog.
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bioRxiv - Cancer Biology 2021Quote: ... 1%IGEPAL CA-630) supplemented with PhosSTOP phosphatase inhibitors and cOmplete protease inhibitor cocktail (both Roche). BCA protein assay (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysates (1 μg/μL) were enzymatically digested with 50 μg/mL pronase E (Roche) at 37°C for 1 hour followed quenching with protease inhibitors and SDS-PAGE loading buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% n-dodecyl β-D-maltoside [DDM] and 1X cOmplete™ EDTA-free protease inhibitor (Roche)) and incubated at 4°C for 30 mins ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of RIPA lysis buffer supplemented with complete protease inhibitor cocktail (cOmplete, EDTA-free [Roche] and phosphatase inhibitor cocktail (PhosSTOP [Roche] ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM MgCl2) supplemented with 1% Triton-X100 and a mini complete protease inhibitor pill (Roche). Lysates were centrifuged at 20,000 × g for 15 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was harvested and protease inhibitors (1 mM PMSF + 1X complete tabs from Roche) were added ...
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bioRxiv - Molecular Biology 2022Quote: ... Cat 15555799) and homogenized at 7000 g for 1 min using a MagNA Lyser (Roche diagnostic) at 4ºC ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 mg/ml phenylmethyl sulfonyl fluoride (PMSF)) supplemented with 1% cOmpleteTM protease inhibitor cocktail (Roche Diagnostics). The tissue lysates were harvested and centrifuged at 12500×g for 15 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM EDTA) supplemented with protease inhibitor cocktail (cOmplete™, EDTA-free, Roche Cat. Nr. 11873580001) and phosphatase inhibitors for 30 min at 4 °C with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 mM TCEP) supplemented with Protease Inhibitor Cocktail (1 tablet/30 ml; complete, EDTA-free, Roche) and sonicated on ice ...
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bioRxiv - Immunology 2022Quote: ... disrupted in 10 ml of RPMI (1%FBS) in the presence of Collagenase D (1X) (Roche) using a MACs dissociator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibodies (1:2000, Roche, 11175041910) in 1% blocking buffer for 4 h at room temperature ...
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bioRxiv - Pathology 2019Quote: ... and 1 μg RNA was used for cDNA synthesis (Transcriptor first strand cDNA synthesis kit, Roche). INF2 was then PCR amplified from cDNA using isoform-specific primers (Supplementary Figure 1 ...
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bioRxiv - Physiology 2020Quote: ... 1%v/v Triton X-100) with protease inhibitors (cOmplete Tablets EDTA-free, EASYpack, Roche, 04693132001). Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled probes were detected using mouse monoclonal anti-DIG primary antibody (Roche; 1:100 dilution) and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... and then washed three times with 1 ml Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, 1 × Roche cOmplete EDTA-free mini protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... PfAP2-G and PfAP2-I were detected with 1 μg/mL rat α HA (Roche 3F10) and 2 μg/mL rabbit α GFP (Abcam ab290) ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissue fragments were digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... Single cell suspensions were then prepared by incubating minced tissue in 1 mg/ml collagenase (Roche) and 50 U/ml DNase I (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to a new tube and combined with 1 μl glycogen (Roche, 10901393001), 40 μl 3M sodium acetate ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA] supplemented with cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche). Clarified cell lysates were incubated with Streptactin Sepharose beads (IBA ...
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bioRxiv - Neuroscience 2019Quote: ... TREM2 ICDs were detected using an anti-HA HRP-coupled antibody (clone 3F10, Roche, 1:250). As a positive control ...
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bioRxiv - Neuroscience 2019Quote: ... Probes were detected using a 1h incubation at RT with anti-fluorescein-POD (1:2500, Roche) and anti-DIG-POD antibodies (1:2500 ...