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Citations for Roche :
4001 - 4050 of 6260 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 10% [v/v] glycerol) supplemented with 1× cOmplete EDTA-free protease inhibitor cocktail tablets (Roche). The resuspended cells were incubated on ice for 30 min with 1 mg/mL egg lysozyme and sonicated (50% amplitude ...
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bioRxiv - Plant Biology 2024Quote: ... the non-specific sites were blocked with 1% Blocking Reagent (Roche, Cat No./ID: 11096176001). The slides were incubated with Anti-Digoxigenin-AP Fab fragment (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... constructs in 6:3:1 weight ratios by X-Treme Gene HP Transfection Reagent (Roche) or the calcium phosphate method ...
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bioRxiv - Pathology 2024Quote: ... the DRGs are incubated at 37°C with 1 mg/mL collagenase A (10103578001, Roche) for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... briefly, cells were then resuspended in lysis buffer (50μg/ml lysozyme, 1×protease inhibitor(Roche), 250 U/ml benzonase ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910). For in situ detection ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used in this study for immunoblot detection were rat anti-HA (1:1000, Roche), mouse anti-myc (1:100 ...
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bioRxiv - Immunology 2024Quote: ... followed by staining of CD3 and PD-1 w with a BenchMark Ultra autostainer (Roche). Tim-3 was stained manually after heat-induced epitope retrieval using the “high pressure” program on an electric pressure cooker (Cuisinart CPC-600 ...
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bioRxiv - Immunology 2024Quote: ... The slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml; Roche, Switzerland) for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were homogenized in EBC-lysis buffer containing 1 mM Complete protease inhibitors cocktail (Roche Diagnostics) and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cultures were pelleted at 1500 g and resuspended in 1 ml Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, 1 × Roche cOmplete EDTA-free mini protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM PMSF and 1 tablet/80 ml of complete EDTA-free protease inhibitor cocktail (Roche)] ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM imidazole) supplemented with 1 mM PMSF and one tablet of complete EDTA free (Roche). After sonication at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3) 2hr RT incubation in blocking solution (5% Sheep Serum, 1% Roche Blocking Buffer in PBST); 4 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were re-probed using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10,000 dilution) to detect ZMPSTE24 and visualized using goat anti-rat IRDye 680RD.
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bioRxiv - Cell Biology 2020Quote: ... 1 μg RNA samples were reverse transcribed using a Transcriptor First Strand cDNA Synthesis Kit (Roche). RT-PCR was performed using a CFX Connect Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then treated with DNase-free RNase (Roche; 5 μg.ml-1; 37°C; 30 min) and proteinase K (250 μg.ml-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were re-suspended in PBS containing 50 μg ml-1 RNase A (Roche Diagnostics) for 30 minutes at 4°C and stained with 50 μg ml-1 PI (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-peroxidase antibodies (1:500, Roche) and revealed using the TSA-Plus Cyanine-3 kit (Perkin-Elmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... for 30 min at 37°C and additionally with 1 mg/ml of collagenase solution (Roche) for 12-18 hr at 37°C with agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA (1 µg) was reversed transcribed with the Transcriptor First Strand cDNA Synthesis kit (Roche). Fragments of fz3 and wg were amplified and used for the templates for synthesizing antisense probes ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed in PBS and the nuclei were counterstained with DAPI(1 μg/ml, Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM TCEP] supplemented with 1 mM PMSF and 1X cOmplete EDTA-free protease inhibitor (Roche). Sonication was performed on ice for 5 cycles with 1 s on ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked for 1 hour at room temperature by x1 blocking reagent (Roche, Germany) dissolved in TBS-T buffer ...
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bioRxiv - Immunology 2019Quote: ... skin samples were digested for 1h at 37°C using 1 U/mL Liberase TM (Roche) and 5 µg/mL DNAse I (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... and adjusted to 1 x 108 tachyzoites/ml with PBS containing protease inhibitors (cOmplete mini, Roche). Parasites were lysed by five freeze-thaw cycles (liquid nitrogen/37°C) ...
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bioRxiv - Immunology 2019Quote: ... with both basic (Cell Conditioner 1) and mildly acidic (Cell Conditioner 2) antigen retrieval methods (Roche, Ventana Medical Systems ...
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bioRxiv - Pathology 2019Quote: ... Lysis buffer mix consisting of 100 μL Lysis buffer (Vial 1, Roche FFPET Isolation Kit # 06650775001), 100 μL dH2O ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with anti-HA-peroxidase antibody (1:5,000; Roche, Cat# 12013819001, clone 3F10) for 1 h at room temperature followed by washing with PBS-T for three times ...
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bioRxiv - Plant Biology 2019Quote: ... Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001, Roche) or anti-actin 1:2,000 (A0480 ...
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bioRxiv - Genomics 2019Quote: ... We ethanol precipitated the flow through by adding 1 μl glycogen (20 μg/μl; Roche, #10901393001) and 2.5 volumes 100% ice cold ethanol followed by incubation at - 20 °C for at least 30 min and then 20 min of centrifugation at 15,000 x g and 4 °C ...
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bioRxiv - Immunology 2019Quote: Spleens were injected with 1 ml of PBS supplemented with Liberase TL (0.125 mg/ml, Roche) and DNAse I (100 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1% Nonidet P-40) in the presence of a protease and phosphatase inhibitor cocktail (Roche) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche), anti-cMYC antibody 9E10 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... 0.5 μM of both forward and reverse primers and 1 × FastStart SYBR Green Master mix (Roche). Steady state levels of transcripts were quantified using primers listed in Supplemental Table 2 ...
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bioRxiv - Biochemistry 2019Quote: ... tissue extracts with 1% Nonidet P-40 in TBS containing protease inhibitors (Complete, Roche Molecular Biochemicals) were mixed with an equal volume of 0.2 M Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Fixed ovaries were lysed in 1 ml of RIPA Buffer (supplemented with complete protease inhibitors (Roche) and RNasin Plus 40U/ml ...
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bioRxiv - Cell Biology 2019Quote: ... left panel) supplemented with protease inhibitors (1 tablet of complete protease inhibitor per 25 ml, Roche, Mannheim ...
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bioRxiv - Genetics 2021Quote: ... transferred on iBlot PVDF Membranes that were probed with anti-GFP (1:1000, Roche ref:11814460001) and custom-made anti-Sir3 (1:10000;(Ruault et al ...
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bioRxiv - Genetics 2019Quote: ... Lysates were diluted in RIPA buffer with 1 µM PMSF and Complete Protease Inhibitor Cocktail (Roche) to a concentration of 1.68 mg/ml and 500 µl was used for capture of biotinylated proteins on streptavidin beads and subsequent mass spectrometry analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated in a solution of anti-Digoxigenin-POD Fab Fragments (Roche, Mannheim, 1:1000). The samples were rocked gently overnight at 4 □ ...
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bioRxiv - Microbiology 2021Quote: Cellular proliferation was evaluated by the colorimetric test WST-1 according to the manufacturer’s instructions (Roche). Cellular viability was assessed by staining the cells with the LIVE/DEAD Fixable Near-IR Dead Cell Stain (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with 1:2000 dilution of anti-DIG-alkaline phosphatase-coupled antibody (Roche, Switzerland) in 10% sheep serum overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... superoxide dismutase activity was measured using a water-soluble tetrazolium salt (WST-1) (purchased from Roche) after drug treatment for 3 days ...
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bioRxiv - Biophysics 2021Quote: ... The following primary antibodies were used at the indicated dilutions: mouse anti-Myc (Roche, 1:3000), goat anti-p75NTR (Neuromics ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blots were performed by standard methods using rat anti-HA (1:1000; clone 3F10, ROCHE), rabbit anti-GFP (1:2000 ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM TCEP containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:1000, 11093274910; Roche) at 4°C for two days ...