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Citations for Roche :
3851 - 3900 of 6260 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1% NP40 detergent and supplemented with a protease inhibitor cocktail mini tablet (complete from Roche) and a phosphatase inhibitor mini tablet (Pierce from Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... The fins were then incubated with anti-dig antibody in blocking solution (1:4000, Roche) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) supplemented with protease inhibitors (cOmplete™, EDTA-free Protease inhibitor cocktail, Roche). For efficient lysis ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were blocked in 1% blocking solution (10 min at RT; Roche Applied Sciences) and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000 ...
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bioRxiv - Cancer Biology 2019Quote: ... media was discarded and cells were incubated with 10 μL of WST-1 reagent (Roche) and 90 μL of growth medium for 4 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibody incubations were performed at room temperature with 1:2,000 diluted anti-DIG Fab (Roche). The embryos were extensively washed before staining reaction ...
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bioRxiv - Microbiology 2019Quote: ... frozen pellets were thawed and resuspended in 1 ml of 3 mg/ml lysozyme (Roche) and 0.4 mg/ml proteinase K (Ambion ...
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bioRxiv - Microbiology 2019Quote: ... 75 U benzonase and 1 Complete Mini EDTA-free protease inhibitor tablet (Roche Applied Science). The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology) ...
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bioRxiv - Developmental Biology 2020Quote: ... Washed samples were incubated with alkaline phosphate-conjugated DIG antibodies (1:4000) (Roche, Mannheim, Germany) for overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... col-LCRM-moeGFP embryos were immunostained with a primary mouse anti-GFP (1/500) (Roche) and secondary biotinylated goat anti-mouse (1/2000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed using a WST-1 cell proliferation assay (Roche, Nonnenwald, Penzberg, Germany). See Supplementary information for details.
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP-40 and 0.5% sodium deoxycholate) with protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) and kept on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... resuspended in 10 mL digestive media (HBSS + 1% HEPES) with Liberase TM (Roche, Cat: 5401119001) or 1000 U/mL collagenase type IV (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM EDTA) and freshly added PMSF (1 mM) and Protease Inhibitor Cocktail (Roche #11836170001). Equal amounts of protein (30 μg ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured as normal and incubated in 100 ng ml−1 Colcemid (Roche, MA) for 2 hours prior to harvest ...
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bioRxiv - Developmental Biology 2021Quote: ... gastruloids were washed once in PBS and digested in 4mg ml−1 dispase I (Roche), 3mg ml−1 collagenase IV (Gibco ...
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bioRxiv - Cell Biology 2020Quote: The following primary antibodies were used: anti-GFP mouse monoclonal at 1:250 (Roche, 11814460001), anti-Cnn Rabbit polyclonal raised against first 660aa of Cnn-P1 (which includes amino acids 35-632 of Cnn-P2 ...
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bioRxiv - Microbiology 2020Quote: ... at a 1:250 dilution and prediluted CD3 rabbit monoclonal antibody (2GV6, Roche Tissue Diagnostics), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% protease inhibitor cocktail set III ethylenediaminetetraacetic acid(EDTA)-free (Roche, Shanghai, China). Protein concentrations were determined using a Pierce BCA Protein Assay Kit (Lineage Cell Therapeutics ...
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bioRxiv - Physiology 2020Quote: Islets were isolated by locally injecting the pancreas with 1 Wünsch unit/ml Liberase (Roche) in HANKS buffer as previously described (22) ...
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bioRxiv - Cell Biology 2020Quote: ... The anti-GFP mouse monoclonal antibody mix (ref. 11814460001, 1:1000) was obtained from Roche. The rabbit anti-PKCζ (ref ...
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bioRxiv - Genomics 2021Quote: ... rRNA was depleted from 1 μg of total RNA with the RiboErase kit (KAPA Biosystems). Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... RNA (1 µg) libraries were constructed using 1llumina TruSeq Stranded mRNA Library Prep Kit (ROCHE) followed by bead size selection (~280 bp ...
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bioRxiv - Neuroscience 2019Quote: ... Mice PSD-95 western-blot PSD-95 monoclonal antibody (1:1000, Roche Group, Nutley, USA), and Trizol ...
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bioRxiv - Neuroscience 2019Quote: ... the samples were immersed into the goat anti-rabbit IgG (1:10000, Roche Group, USA) to incubate at room temperature for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... 1% Triton X-100) containing protease inhibitors (cOmplete protease inhibitor cocktail, EDTA-Free, Roche Diagnostics). Cells were then lysed mechanically with scrapers ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche). The next day the embryos were washed in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... S.p.a) and midazolam (0,5 mg kg −1) (Dormicum®, 5 mg/ml, Roche Pharma, Switzerland) for IM premedication before the start of the procedure ...
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bioRxiv - Developmental Biology 2020Quote: ... Animals were then incubated with an appropriate antibody: 1:1000 anti-DIG-POD (Roche 11207733910), 1:1000 anti-FITC-POD (Roche 11426346910 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MgCl2 pH 6.8) supplemented with protease inhibitor cocktail (Complete mini EDTA free, Roche) and 1 mM DTT ...
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bioRxiv - Developmental Biology 2020Quote: ... NP-40 1% and Na-deoxycholate 0.25 %) supplemented with protease inhibitor cocktail (Roche, Mannheim, Germany). For immunoprecipitations ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibodies used in this study include mouse αGFP (1:1000, 0.4 mg/ml, Roche), rabbit αGFP (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibodies used in this study were mouse αGFP (1:2000, 0.4 mg/ml Roche), rabbit αREX1-repeat (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 % Triton-X100) supplemented with EDTA-free protease inhibitor cocktail (c0mplete Mini EDTA-free, Roche) and phosphate inhibitors (60 mM β-glycerophosphate ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were detected using an HA-HRP antibody (12-013-819; 1:3,500; Roche).
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bioRxiv - Neuroscience 2021Quote: ... Cells were then permeabilized in 1 % bovine serum albumin (BSA; Roche Diagnostics GmbH, Germany, 10735078001) in PBS-Triton 0.1 % solution for 5 min and blocked in 1 % BSA in PBS at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were incubated with 1:4,000 diluted alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche, 11093274910) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... pH 8) containing 1 mM PMSF and Complete™ EDTA free protease inhibitor cocktail (Roche). Nuclei were removed by centrifugation for 1 min at 6,000 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol) supplemented with 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche) per 50 mL and lysed using a Dounce homogenizer (10 strokes with a loose plunger and 15 strokes with a tight plunger) ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol) supplemented with 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche) per 50 mL was used ...
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bioRxiv - Microbiology 2021Quote: ... and 25 Abs260 ribosome units (1 A260 = 12 μg/μL) were digested with MNase (Roche). Digested samples were carefully loaded onto sucrose gradients and centrifuged at 124,700 × g for 8 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and ethanol precipitated with human Cot-1 DNA (Roche, Germany) and herring sperm DNA (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Livers and lungs were treated with collagenase D (for lung digestion, 1 mg/ml, Roche) or collagenase Type 4 (for liver digestion ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 0.2 M HCl) freshly complemented with 1× Proteinase Inhibitor Cocktail (Roche) and 10 mM N-ethylmaleimide (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... 1% Igepal CA-630 and 150mM NaCl) supplemented with complete protease inhibitor cocktail (Roche; 11697498001) and PhosSTOP (Roche;4906845001) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL Turbo nuclease (Accelagen) and one tablet of Complete EDTA-free protease inhibitor (Roche) (Cormier et al. ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed in NP-40 lysis buffer containing 1× cOmplete Protease Inhibitor Cocktail (Roche) and 1× Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... diluted 2-fold with PBMC culture media and supplemented with 10% WST-1 reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Overt diabetes was confirmed by testing for fasting glycemia >250 mg.dL-1 (Accu-Check; Roche).
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bioRxiv - Immunology 2021Quote: ... and resulting amplicons were sequenced on a GS FLX 1 Sequencing system (Roche, Pleasanton, CA). Repertoire was done using IMGT/High-V-Quest and associated RStudio package scripts ...