Labshake search
Citations for Roche :
3951 - 4000 of 4072 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: TUNEL staining was performed according to the manufacturer’s protocol with minor modifications (In Situ Cell Death Detection Kit, TMR red; catalogue number 12156792910; Roche, Mannheim, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Pathology 2023Quote: Cells or tissues were homogenized in RIPA buffer supplemented with protease inhibitors and phosphatase inhibitor cocktails (Roche Diagnostics, Indianapolis, IN, USA). Western blot analysis was performed according to the routine procedure ...
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bioRxiv - Neuroscience 2023Quote: ... 300,000 cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 2X with cold DPBS++ and RIPA buffer (Pierce #89900) containing complete EDTA-free protease inhibitor cocktail (Roche #11873580001) was added to wells ...
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bioRxiv - Cell Biology 2023Quote: ... Infected red blood cells were lysed 10 min on ice with 0.1% saponin in PBS complemented with 1x Protease Inhibitors Cocktail (Roche; PBS+PI). Parasites were pelleted and washed with PBS+PI to remove haemoglobin ...
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bioRxiv - Cell Biology 2023Quote: The tissues from treated mice and HFs were used for TUNEL apoptotic detection (In Situ Cell Death Detection Kit, TMR Red, Roche 12156792910) After fixation and antigen retrieval procedures ...
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bioRxiv - Genomics 2023Quote: ... sgRNA- or cDNA-expressing vectors and lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293T cells using XTremeGene 9 transfection reagent (Roche #636478700). The supernatant containing virus was collected 48 h after transfection and passed through a 0.45-µm filter ...
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bioRxiv - Cancer Biology 2024Quote: ... HSJD-DIPG-012 and HSJD-DIPG-014 cell lines by real-time quantitative QRT-PCR on a LightCycler® 480 instrument (Roche) using a RT² Profiler PCR Array (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (used only for CDKN1A ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in mHBSS at a density of 107 cells/ml and pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM in a BSA-coated centrifugation tube ...
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bioRxiv - Cell Biology 2024Quote: ... HL-60s were differentiated into a neutrophil-like state by taking an aliquot of cells in their culturing medium and supplementing with an equal volume of Nutridoma-CS (Roche #11363743001) and DMSO diluted in RPMI such that that the final concentrations were 0.2 million/mL HL-60 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed and lysed in radioimmunoprecipitation assay buffer (RIPA) buffer supplemented with Complete® protease inhibitor cocktail (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail II (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: Cellular proteins were obtained by extracting whole-cell lysates using RIPA lysis buffer enhanced by addition of phosphatase and protease inhibitors (Roche 11836153001). To remove debris ...
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bioRxiv - Cell Biology 2024Quote: The cells were lysed in cold Lysis buffer supplemented with cOmplete mini EDTA-free protease inhibitor cocktail (Cat. No. 11836170001, Roche, Switzerland) and phosphatases inhibitor PhosSTOP™ (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... Fixed embryos were homogenized in 2 ml cell lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.3% NP-40, 1x Roche Complete protease inhibitors cocktail) with a Dounce Homogenizer on ice and centrifuged 5 min 2,300g at 4°C ...
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bioRxiv - Pathology 2019Quote: ... and plasma HIV-1 RNA levels were determined in HIV-1-infected persons at the Makerere University-John Hopkins University laboratory using standard cell sorting techniques and the Amplicor HIV-1 monitor test (Roche, version 1.5), respectively ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was obtained from astrocytoma cell line 1321N1 using TRIzol and reverse-transcribed with Transcriptor Reverse Transcriptase according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). Full-length human PFKFB3-4 was generated by standard PCR using primers PFKFB3-4 reverse and PFKFB3-4 forward (Table S2) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed with 4% SDS lysis buffer (4% SDS, 150 mM NaCl, 50 mM triethanolamine pH 7.4, Roche protease inhibitor, benzonase). Protein concentrations were determined by the BCA assay (Pierce) ...
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bioRxiv - Microbiology 2019Quote: ... The PBMCs were fused with cells from the mouse–human fusion partner cell line SPYMEG (Medical & Biological Laboratories, Nagoya, Japan) at a ratio of 10:1 with polyethylene glycol (Roche, Basel, Switzerland). Fused cells were cultured in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Cell Biology 2021Quote: Two days after transfection, cells were lysed with RIPA Lysis Buffer (P0013C, Beyotime) supplemented with 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min ...
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bioRxiv - Physiology 2022Quote: ... n=3/genotype) were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to visualize cell apoptosis/death (Roche, Basel, Switzerland). Sections were counterstained with DAPI and visualized using epifluorescence (Nikon Eclipse Ni E800) ...
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bioRxiv - Pathology 2022Quote: We detected DNA fragmentation resulting from apoptotic signaling cascades with the In Situ Cell Death Detection Kit Fluorescein (Roche, 11684795910, Basel, Switzerland) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
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bioRxiv - Microbiology 2020Quote: α-GDV1-GFP-DD and α-Pfs16 IFAs were performed with methanol-fixed cells using mouse mAbs α-GFP (1:200) (Roche, #11814460001) and α-Pfs16 (1:500 ...
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bioRxiv - Biochemistry 2021Quote: Cell-free histone levels were assessed in plasma from healthy controls and COVID-19 patients using a photometric enzyme immunoassay (Cell Death Detection ELISAPLUS kit, Roche Applied Science) that measures histone-associated DNA fragments according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Nucleic acids from these 200μL mosquito pools and from 100μL venous and finger prick whole blood samples in RNAprotect Cell Reagent were isolated using the bead-based MagNAPure LC automatic extractor (Total Nucleic Acid Isolation Kit—High Performance, Roche Applied Science) and eluted in 50μL of water ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were transfected into HeLa cells growing on 35 mm glass bottom dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Immunology 2020Quote: ... Ice cold 1.5mM EDTA was slowly poured into the lysozyme-treated cells and 1x protease inhibitor was added (Roche cat no. 04693159001). The suspensions were sonicated in an ice/water slurry for eight minutes total with the following parameters ...
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bioRxiv - Neuroscience 2020Quote: The extent of cell death following MPP+ treatment with and without LMX1B overexpression was assessed by using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) method (In situ cell death detection kit, TMR red, Roche, Cat# 12156792910), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: The cell lysates were extracted with RIPA lysis buffer that contained a protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Basel, Switzerland). Equivalent concentrations of protein were separated using gel electrophoresis and were then transferred to a PVDF membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... a debris removal step was performed (Miltenyi #130-109-398) and blood cells were removed using a blood lysis buffer (Roche 11814 389001).
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were harvested 20 hours post-infection (hpi) and suspended in RIPA buffer with protease and phosphatase inhibitors (Roche, Thermo Fisher). Samples containing 10-20 µg protein were boiled for 20 min in sample buffer ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... Ganglia were mechanically disrupted with a pipette tip until obtaining a single cell suspension in culture media supplemented with DNAse I grade II at final concentration 3mg/ml (Roche, Cat# 104159), which was centrifuged at 1300 rpm (302 rcf ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were treated with the oligonucleotide mixed with DOTAP liposomal transfection agent (35 μg/ml) (#11811177001, Roche Diagnostics GmbH, Mannheim, Germany) for 3 to 6 hrs (16) ...
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bioRxiv - Genomics 2021Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) per gram of cells in the presence of protease inhibitors (Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Suspensions were flash frozen in droplets and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Cell Biology 2021Quote: A reference sample was generated by lysing TK6 cells in DPBS with 2% SDS and cOMPLETE protease inhibitors without EDTA (Roche, 1x concentration) at 70 °C ...
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bioRxiv - Microbiology 2020Quote: ... These mutant viruses were made using HEK-293/17 cells that were transfected with 6 μg of viral DNA using Xtreme-GENE HP (Roche, Basel, Switzerland) Transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... an equimolar amount of DMSO was used to stimulate the cells in triplicate wells and as positive controls phytohemagglutinin (PHA, Roche, 1μg/ml) and a cytomegalovirus MP (CMV ...
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bioRxiv - Immunology 2021Quote: ... it was added 50 µL of fresh TUNEL reaction mixture composed of 5 µL of enzyme solution mixed with 45 µL of labeling solution (In Situ Cell Death Detection kit, POD, ROCHE, version 15.0) for 1 hour at 37 °C ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into HeLa cells growing on 100 mm dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols and processed for TEM at 18 hours post transfection ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Neuroscience 2021Quote: The culture medium was collected and cells were lysed in RIPA buffer supplemented with 1x cOMPLETE protease inhibitor cocktail (Roche, Basel, Switzerland). Cell lysates and medium for phosphorylated tau and total tau Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Developmental Biology 2022Quote: ... in situ terminal deoxynucleotidyl transferase deoxyuridin triphosphate nick end labeling (TUNEL) assay was performed using in situ cell death kit from Roche (Basel, Switzerland).
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bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
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bioRxiv - Microbiology 2022Quote: ... genes was performed using reagents and equipment supplied by Advanced Cell Diagnostics (ACDBio, Hayward, CA) and Ventana Medical Systems (Roche, Tuscon, AZ). The in situ hybridization RNAscope® probes where designed based on the simian HAV Macaca/Germany/2021 ...