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Citations for Roche :
351 - 400 of 451 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... pH 7.5) containing protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each 1 tablet per 15 mL, Roche, Basel, Switzerland), and diluted in 6×SDS-PAGE sample loading buffer (Beyotime ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Amplifications were performed using 15 cycles with Nextera-style dual indexing primers (Kircher et al., 2011) and KAPA HiFi Hotstart ReadyMix (Kapa Biosystems). Library concentrations were measured with Qubit dsDNA High Sensitivity Assay kit.
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
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bioRxiv - Microbiology 2021Quote: ... RBCs were lysed with 0.15% (w/v) saponin and parasite pellets washed three times with PBS and cOmplete™ mini EDTA-free protease inhibitor (Roche) at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were boiled 15 min at 95°C to gelatinize the starch and hydrolyzed with α-amylase and amyloglucosidase (Roche) to release the glucose blocks ...
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bioRxiv - Microbiology 2021Quote: The following agents have been used in the study: cotrimoxazole (Bactrim, sulfamethoxazole and trimethoprim 150/ 15 mg/ mL; Roche, Basel, Switzerland), artesunate (10 mg/ mL ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 0.5 mM PMSF, 1 mM DTT and 1×Complete inhibitor tabs, Roche, for 15 min), then treated with 10% NP40 and vortexed ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction contained a 5μl of a 1 in 10 dilution of genomic DNA and was carried out in quadruplicate in a volume of 15 μl in a 384-well LightCycler® 480 PCR plates (Roche), sealed with LightCycler® 480 sealing foil (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription products were then amplified by quantitative PCR (95 °C, 5 minutes; 95 °C, 15 seconds; 60 °C, 60 seconds; 40 cycles) (LightCycler® 480, Roche) using TaqMan Universal PCR Master Mix and specific probes for miRNAs ...
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bioRxiv - Plant Biology 2022Quote: ... RT was performed with 5 µg of total mRNA using Transcriptor Reverse Transcriptase and oligo (dT)15 primer (Roche, Mannheim, Germany). QRT-PCR was run on a LightCycler 480 (Roche ...
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bioRxiv - Genomics 2020Quote: ... The six STARR-Seq RNA replicate libraries and a STARR-Seq Plasmid DNA input library (amplified as before but with 20 ng DNA input and 15 PCR cycles) were normalized with the KAPA Library Kit (KAPA Biosystems) and sequenced on an Illumina NextSeq with 150-bp paired-end reads using standard protocols.
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bioRxiv - Microbiology 2022Quote: ... followed by 40 cycles of amplification (95°C for 15 sec, 60°C for 30 sec) (Roche LightCycler 96, Roche Diagnostics). For absolute quantification ...
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bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
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bioRxiv - Genomics 2023Quote: ... and Illumina PCR-free libraries were prepared from 700 ng DNA using the KAPA Hyper prep kit and unique dual-indexed adapters (5 µL of a 15 µM stock) according to the supplier’s protocol (Roche, Basel, Switzerland). The library concentration and size distribution were assessed on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... mycelia were resuspended in lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg/mL lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany)) and incubated at 37°C for 20 min ...
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bioRxiv - Genomics 2019Quote: The gDNA libraries were prepared using a SeqCap EZ Human Exome Library v2.0 (Roche, Basel, Switzerland). Sequencing was performed with 100-bp paired-end reads by the HiSeq2000 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal Probe Library (UPL) Probefinder software for human (Roche, version 2.53) or using previously published sequences and adjusted to be intron-spanning ...
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bioRxiv - Immunology 2019Quote: For the in vitro cell stimulation and maintenance reagents were as follows: human IL-2 (Roche); IL-21 (PeproTech) ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for the immunostaining include human ERα rabbit monoclonal antibody (Roche Diagnostics, 790-4325), human PR rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science) according to the manufacturer’s instructions and underwent 2 × 151 base-pair sequencing on Hiseq 4000 (Illumina ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were subsequently dual indexed and amplified for 15 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-µl reactions following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2019Quote: Each first strand cDNA reaction was supplied with 15 µl of PCR mix containing 1x KAPA HiFi HotStart Ready Mix (Kapa Biosystems, KK2601), 1 µM IS PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Genomics 2021Quote: ... For a 15 ul reaction 1 ul of undiluted cDNA was combined with 1x FastStart Universal Probe Master Mix with Rox (Roche Diagnostics, Australia), 50 nm of the forward and universal reverse primer ...
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bioRxiv - Immunology 2020Quote: ... pursued by 95°C for 3 minutes and then 45 cycles at 95°C for 15 seconds and 58°C for 30 seconds using a LightCycler 480 Real-Time PCR system (Roche, Rotkreuz, Switzerland). The primers and the probes were designed against the E gene (20).
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bioRxiv - Cancer Biology 2023Quote: All experimental cells were subjected to extraction of total proteins in a lysis buffer containing a protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each tablet per 15 mL buffer, Roche, Basel, Switzerland). The proteins in cell lysates were denatured at 100°C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: 15 ng of each patient’s cDNA library were amplified using the KAPA HiFi HotStart Uracil+ ReadyMix 2x (Kapa Biosystems, cat. no. KK2801) with 0.5 µM final concentration of custom-primers (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Immunology 2021Quote: Total RNA was retrotranscribed and cDNA was quantified using the Universal Human Probe Roche library (Roche Diagnostics). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µL egg extract was diluted in 16.5 µL reaction buffer (80 mM β-glycerophosphate, 20 mM EGTA, 15 mM MgCl2, 10 uM okadaic acid, 1x protease inhibitor (Roche, cOmplete #11873580001), 100 µM ATP ...
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bioRxiv - Molecular Biology 2021Quote: ... sections were fixed with 4% PFA (PierceTM, 28908) in PBS for 15 minutes at room temperature and permeabilized with PCR grade recombinant Proteinase K (Roche Applied Science, 3115887001) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR reactions were run for 50 cycles (95 °C for 15 s and 60 °C for 45 s) on a LightCycler 480 (Roche Diagnostics, Mannheim, Germany). Each sample was examined in three technical replicates and dissociation curves were analysed to verify the specificity of each amplification reaction ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then dried and cut into small pieces before the pronase treatment (15 mg pronase per hemisphere, (Roche, cat. no. 11459643001) in 0.1 M Trizma hydrochloride (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Real-time quantitative PCR (qPCR) was performed in accordance with MIQE-guidelines (15) using a LightCycler® 480 Real-Time PCR Instrument (Roche, Mannheim, Germany) in a 384-well plate format ...
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... freshly resected human samples were cut into small fragments and digested with 0.1 mg/ml Liberase TL (Roche) and 0.1 mg/ml DNase (Roche ...
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bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... human vimentin immunohistochemical staining of the paraffin sections was performed by using the Ventana Discovery XT instrument (Roche) with Ventana DAB Map detection Kit (760-124 ...