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Citations for Roche :
101 - 150 of 451 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM MgOAc, 15 mM Tris-HCl pH 7.4, 0.5% NP40, 1 mM DTT, 1 μl RNAsin, Roche protease inhibitors without EDTA). The cells were lysed by one freeze-thaw cycle in large volume (15–50 ml ...
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bioRxiv - Immunology 2019Quote: ... RNA was reverse transcribed with pd(T)-15 (Roche) and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 15 mM NaCl and protease and phosphatase inhibitors (Roche) for protein extraction ...
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bioRxiv - Physiology 2019Quote: ... BDM 15 and 200UI/mL collagenase A (Roche Diagnostics) plus bovine serum albumin 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... BDM 15 and 200UI/mL collagenase A (Roche Diagnostics) plus 0.5% bovine serum albumin ...
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bioRxiv - Genetics 2023Quote: ... 15 ul of 2X FastStart™ PCR Master (Roche), 2 ul of DMSO ...
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bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... 15 μl of 20 mg/ml RNase A (Roche 10109169001) was added to the samples that were then quickly vortexed and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... containing 15 mg ml-1 lysozyme (Roche or Sigma-Aldrich) and 10 μl proteinase K (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... was added to 15 mL DIG EasyHyb™ Buffer (Roche) to reach a final concentration of 25 ng/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... with micrococcal nuclease (MNase,15 units per tube; Roche, #10107921001) in an MNase buffer (0.3 M sucrose ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was dissociated in papain (15 U/mL, Roche) containing 1 mM L-cystein ...
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bioRxiv - Biochemistry 2023Quote: ... 15 U/mL lactate dehydrogenase (Roche Diagnostics GmbH, Mannheim, Germany), 8.4 U/mL pyruvate kinase (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
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bioRxiv - Plant Biology 2021Quote: ... His-tagged AtTPL188 (wt and mutants) bacteria pellets were resuspended in buffer A with EDTA-free antiprotease (Roche). The soluble fractions recovered after sonication were passed through a Ni-sepharose (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The His-tagged-TRF2TRFH protein was purified in presence of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) by using Protino ® Ni-TED 2000 Packed Columns (Macherey-Nagel ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged rhFGFb was detected using a primary anti-HISx6 mouse polyclonal antibody (Roche Molecular Systems, Inc., USA) or anti-FGF polyclonal antibody (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Human IL-2 (TECIN™ teceleukin, ROCHE, was generously provided by the NCI Biological Resources Branch ...
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bioRxiv - Molecular Biology 2021Quote: ... and human genomic DNA (Roche Diagnostics, Germany) were used as templates for the experiments in Figure 2 and S1a ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD20 human Ab (obinutuzumab, Roche). Relevant negative controls were performed using isotype antibodies and cells incubated with anti-CD20 human and anti-CD20 mouse antibody served as a positive control ...
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bioRxiv - Immunology 2023Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 15 mM MgCl2 and 0.3 U/µl DNase I (Roche Diagnostics) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM CaCl2 and 15 units of S7 micrococcal nuclease (Roche). Lysates were sonicated for 10 seconds at low power followed by incubation on ice for 30 minutes and clarification by centrifugation at 13,000 x g for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The bacteria were stained with 15 μg/mL of DAPI (Roche) for 30 min at room temperature and imaged using a 63× oil immersion lens on a Zeiss Axio Imager.M2 microscope.
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified products were subjected to second strand synthesis using 0.3 µM of second strand biotinylated primer and the KAPA Hi-Fi hot start ready mix (KK2601, Roche). The second strand reaction was carried out at 95 °C for 3 minutes ...