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Citations for Roche :
251 - 300 of 451 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... We then added 15 µl PCR master mix containing 12.5 µl KAPA HiFi HotStart ReadyMix (KAPA Biosystems)) and 0.5 µl 10 µM PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT) ...
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bioRxiv - Genetics 2020Quote: ... cDNA amplification was done for 15 cycles from pools of 2,000 beads using HotStart ReadyMix (Kapa Biosystems) and the SMART PCR primer ...
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bioRxiv - Cell Biology 2021Quote: ... and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml) (Roche). Damaged livers (48h-72h after APAP injection ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 15 minutes with 4 μL of X-treme Gene HP DNA transfection reagent (Roche). Transfected cells were selected for by hygromycin resistance ...
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bioRxiv - Biophysics 2022Quote: ... then diluted to 0.5 μM in KMEH + 15 KH2PO4 supplemented with 0.01% Nonidet P40 (NP40) substitute (Roche), an additive which we have found improves our ability to achieve thin vitreous ice films ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 mM EGTA; 1.5 mM MgCl2; 150 mM KCl; 15% glycerol; 0.075% NP-40 to which Roche cOmplete™ Mini tablets containing a protease inhibitor cocktail were added just prior to use) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... washed in KPBS and incubated for 15 minutes at 37 °C in presence of proteinase K (Roche) at 0.01 mg⁄mL in an appropriate buffer (0.1M Tris–HCl pH 8.0 ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% SDS) at 50°C for 15 min and finally washed with 20 ml washing buffer (Roche) at room temperature for 5 min ...
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bioRxiv - Genetics 2023Quote: ... We PCR-amplified bisulfite-treated DNA with 15-16 cycles with KAPA HiFi Uracil polymerase (Kapa Biosystems). We used Illumina TruSeq unique index adapters for PCR amplification and multiplexing of the libraries ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2 % SDS and 15 mM EDTA) in the presence of protease inhibitors (Roche, Complete Mini, EDTA-free). Blotting was conducted using the Trans-Blot Turbo Transfer System (Biorad) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl of target RNA were incubated for 15 min with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) was used in the immunostaining assay.
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bioRxiv - Cancer Biology 2021Quote: ... Pooled sample libraries were hybridized with SeqCap EZ Human Exome v3.0 probes (Roche) for WES ...
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... 3×10−4 M MTG and 300 μg/ml human transferrin (Roche, 10652202001)) in a triple vent petri 10 cm dishes (Thermo fisher ...
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bioRxiv - Immunology 2024Quote: Human monocytes were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Penicillin/Streptomycin and 20 IU human IL-2 (Roche Diagnostics, Mannheim, Germany) for 5d ...
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bioRxiv - Cell Biology 2021Quote: ... 350 mM NaCl, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 0.1% Triton X-100, 0.05% 2-ME, 1x Roche protease inhibitors ...
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bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
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bioRxiv - Cell Biology 2022Quote: ... oocytes were then roughly separated in bundles of 15-20 oocytes and treated with 1mg/ml Liberase (Roche) to remove the surrounding tissue.
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bioRxiv - Genetics 2021Quote: ... Muscle was then digested in a 15 ml Falcon tube containing 2.4 U/ml Collagenase D (11088882001, Roche), 12 U/ml Dispase II (04942078001 ...
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bioRxiv - Biophysics 2023Quote: ... 1a) in a 27°C water bath for 15 minutes and then quenched with 0.5 mM PMSF (Roche). Samples were analyzed on a Coomassie stained SDS-PAGE PAGE gel ...
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bioRxiv - Biophysics 2024Quote: ... A second PCR amplification was performed with KAPA HiFi (KAPA Biosystems; 1-μL qPCR template, 15 cycles maximum). We amplified corresponding regions of pSC101_TetR_specR with primers that linearized the backbone ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines were provided by the Roche Non-Clinical Biorepository from Roche Basel or the Roche-Innovation Center Zurich (RICZ) ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... Evaluation of the ratio Leishmania/human DNA was performed by qPCR on LightCycler480 (Roche) using SensiMix SYBR No-ROX (Bioline ...
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bioRxiv - Genetics 2020Quote: ... and hybridized to human exome probes using the SeqCap EZ Prime Exome kit (Roche). The resulting exome libraries were sequenced with paired-end 150 bp Illumina reads on the HiSeq or NextSeq platforms at Admera Health (South Plainfield ...
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bioRxiv - Genetics 2021Quote: Human BE5.1 was amplified using high fidelity PCR (Expand high fidelity system, Roche, UK) from human DNA (Cambio ...
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bioRxiv - Genomics 2022Quote: ... fourteen non-coding regions of interest were PCR amplified from human genomic DNA (Roche) using the Phusion High-Fidelity PCR Kit (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were separated on 10%-15% SDS-PAGE gels and transferred to PVDF membranes (Roche, 03010040001). The membranes were probed with the indicated primary antibodies followed by the appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 mM EDTA, 15% glycerol, 2 mM MgCl2, 1 mM DTT, 1 mM PMSF, and protease inhibitor cocktail [Roche]). Following clarification by centrifugation ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
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bioRxiv - Cell Biology 2019Quote: ... ∼100 µl glass beads and 100 µl of lysis buffer (50 mM Tris-HCl pH 8.0, 1 mM EDTA, 15 mM Tris pH 9.5, 3 mM DTT, 1X cOmplete EDTA-free inhibitor cocktail [Roche]) were added to each dried pellet ...
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bioRxiv - Genetics 2019Quote: Tissues from 15-30 animals were dissected in CST lysis buffer (Cat#9803) containing 2x protease inhibitor (Roche, 04693159001) and 3x phosphatase inhibitor (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... boulardi venom reservoirs were dissected individually in 15 µl of insect Ringer supplemented with protease inhibitors cocktail (PI; Roche), mixed with an equivalent volume of Laemmli reducing buffer and heated (95°C ...
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... diluted with an equal volume of IP buffer (150 mM HEPES-KOH pH 6.8, 150 mM K2OAc, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 1% NP-40, 1x Roche protease inhibitor cocktail and 1 x phosSTOP cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized with 0.5% TritonX- 100 for 15 minutes at room temperature and incubated with 1mg ml−1 DNase I (Roche) for one hour at 37 °C followed by incubating with anti-BrdU (BU20A ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed 2x in PBS + 0,1% Tween-20 (PBS-T) followed by 15 min permeabilization in 1- μg/ml proteinase K (Roche) and 3 washes in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... The Glu-C treated gel pieces were then dried by SpeedVac and rehydrated in a small volume (15 μL) of 50 mM AmBic supplemented with 0.5 μg of trypsin (Roche) at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Microbiology 2021Quote: ... cells were collected by centrifugation (4,650 × g, 20 min) and resuspended in 15 ml of TNG buffer supplemented with Protease Inhibitor Cocktail (Roche). Cells from the SPA tagged protein cultures were mixed with the control WT or HA-tagged protein cultures at a 1:1 ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... briefly rinsed with 50 mM AmBic and rehydrated in a small volume (15 μL) of 50 mM AmBic supplemented with 0.5 μg of chymotrypsin (Roche) or Glu-C (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Cells were pelleted at 500g for 5 min and lysed in 1 mL of ice-cold lysis buffer (25mM Tris pH 7.4, 150mM NaCl, 15 mM MgCl2, 1mM DTT, 1X cOmplete EDTA-free protease inhibitor (Roche), 100µg/mL CHX and 1% Triton X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 60 mM KCl, 15 mM NaCl, 1 mM CaCl2, 0.9% Triton X-100, and 13 protease inhibitor cocktail [Roche]). After homogenization ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.6; 15 mM NaCl; 10% glycerol; 0.5% Tween 20; 10 mM imidazole; 1 mM DTT; 20 mM NaHCO3; Roche protease inhibitors cocktail without EDTA) ...
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bioRxiv - Genomics 2023Quote: TP pre-capture libraries were prepared as previously described [15] using the KAPA HyperPlus kit with UDI adapters (Roche). 17.5µL DNA was used as input for fragmentation for eight minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Tissues were washed in three changes of 0.2× SSC at 44°C for 15 minutes each and blocked in 4×SSC containing 1% blocking reagent (Roche) in 4× SSC for 1 hour at RT ...
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bioRxiv - Genomics 2023Quote: ... samples were minced and 0.15-0.2g of the minced tissue was transferred into the digestion mix (1.07 wU/ml Liberase DH (Roche, 5401054001), (70 U/ml ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonadal tissues were washed three times in 0.2× SSC at 50 °C for 15 min each and transferred to 4× SSC containing 1% blocking reagent (Roche) for 1 h at RT ...