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Citations for Roche :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... End point cell proliferation was assayed with ELISA 5-bromo-2′ -deoxyuridine (BrdU) kit from Roche Diagnostics (Sigma– Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... and Complete Protease Inhibitor Cocktail (Roche, 11836145001). The lysed cells of every condition were pooled together (six dishes per condition were seeded) ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.113 µg/µL Proteinase K (Roche, 3115828001) for 45 min at 45°C ...
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bioRxiv - Biophysics 2024Quote: ... 0.5-2 M of urea (depending on the construct) and one protease inhibitor cocktail tablet (Roche). Following sonication on ice (10 min at 40 % power ...
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bioRxiv - Molecular Biology 2024Quote: ... then Lysis Buffer 2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Roche cOmplete™ ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× cOmplete EDTA-free protease inhibitors (Roche) and 5 mM benzamidine hydrochloride ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 1000x DTT (1M) and Protease Inhibitor Cocktail (Roche, COEDTAF-RO). Cells were lysed for 5 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Protease Inhibitor Cocktail (Roche, COEDTAF-RO) and 0.04 mg/ml DNaseI ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were scraped from the plate and lysed with 0.5% NP-40 buffer (10 mM Tris-HCl, 10 mM NaCl, 0.5% NP-40, freshly added Roche cOmplete protease inhibitor cocktail) for 15mins at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The tumor tissue was finely minced and digested for 25 min with 0.025% collagenase A (cat no. 10103586001; Roche) and 1 mM EDTA in PBS at 37°C under frequent agitation and trituration ...
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bioRxiv - Microbiology 2024Quote: ... using a LightCycler480 (Roche) with primers ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Microbiology 2024Quote: ... Broncholalveolar lavage (BAL) fluids were obtained by intratracheal injection of 2 × 1 ml PBS supplemented with protease inhibitors (Roche). Serum was collected by intracardiac puncture and separated from whole blood in Z-Gel microtubes (Sarstedt) ...
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bioRxiv - Microbiology 2024Quote: ... pH 7.5) for 15 min at 37°C and blocked with blocking solution (#11096176001, Roche) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:10,000, Sigma #F1804-5MG; anti-GroEL, 1:10,000, Sigma; or anti-GFP, 1:10,000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Microbiology 2024Quote: ... on a LightCycler 480 II (Roche Life Sciences). Each qPCR run included a standard curve of 10-fold serial dilutions of a known concentration of R ...
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bioRxiv - Microbiology 2024Quote: ... Signals were visualized by CDP-star (#11759051001, Roche) on a ChemScope imaging station and quantified using ImageJ Software.
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bioRxiv - Microbiology 2024Quote: ... membrane was hybridized with 75 mU/ml Anti-Digoxigenin-AP (#11093274910, Roche) in blocking solution for 60 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were treated with Lumi-LightPLUS Western Blotting substrate (Roche, Switzerland) for signal development and visualized using a ChemiDoc XRS+ station (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... double-stranded complementary DNA (cDNA) was synthesized using the cDNA synthesis system (Roche, UK) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... 10μl /well cell proliferation reagent WST-1 (Roche 05015944001) was added ...
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bioRxiv - Microbiology 2024Quote: ... EDTA-free protease inhibitor cocktail (Roche). After high-speed centrifugation at 20,000 rpm for 2 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... EDTA-free protease inhibitor cocktail (Roche). Lungs were homogenized using a mixer mill ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% glycerol) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48,000g for 45 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with 30 µL lysis buffer (final concentration: 50 μg/ml lysozyme, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA containing either the on-target or off-target sites was amplified with KAPA HiFi HotStart DNA polymerase (Roche) or SUN-PCR Blent (SUN GENETICS ...
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bioRxiv - Molecular Biology 2024Quote: ... activity was measured in a kinetic assay,39 using a commercial kit (Sentinel Diagnostics 17234H) adapted for use on either a Cobas Fara or Mira analyser (Roche Diagnostics). Intra-assay and inter-assay precision were CV < 4% and CV < 8% respectively.
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bioRxiv - Microbiology 2024Quote: ... The pellet was subjected to enzymatic lysis with 90 µl of 50 mg/ml lysozyme (Roche, Mannheim, Germany) and 50 µl of 5 KU/ml mutanolysin from Streptomyces globisporus ATCC 21553 (MilliporeSigma ...
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bioRxiv - Microbiology 2024Quote: ... was performed on a LightCycler (Roche, Basel, Switzerland) based on SYBR Green technology ...
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bioRxiv - Microbiology 2024Quote: ... 5′-32P-end-labeled deoxynucleotide primer were hybridized to complementary regions of rRNA (16S nucleotides 1494–1510) and extended with AMV reverse transcriptase (Roche). Extension products were run on denaturing polyacrylamide/urea gel and visualized by autoradiography using a Typhoon PhosphorImager (Amersham Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 nM ADP-HPD (a PARG inhibitor; sigma) and 1× protease inhibitor cocktail (Roche)] and incubated for 30 min on ice with gentile vortexing and then centrifuge at maximum speed for 15 minutes at 4°C in microcentrifuge to remove the cell debris ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× protease inhibitor cocktail (Roche), 10 μM PJ34 (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 tablet per 50 ml complete EDTA-free protease inhibitors (Roche). The cells were subsequently disrupted using an Emulsiflex-C5 high-pressure homogenizer (Avestin ...
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bioRxiv - Microbiology 2024Quote: ... and 10% glycerol) supplemented with the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche), frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from the enrichment broth using the MagNA Pure 96 platform (Roche Diagnostics, Almere, the Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... on a LightCycler 96 (Roche) using LightCycler software v1.1.0.1320 ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies used were prepared in 2% BSA (diluted in PBS) and used at the following concentrations: rat monoclonal anti-HA (1:1000, 3F10 Roche), mouse monoclonal anti-myc (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used in this study for immunoblot detection were rat anti-HA (1:1000, Roche), mouse anti-myc (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with cOmplete Mini protease inhibitors mix (Roche)) and incubated overnight at 4°C on a rotating wheel ...
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bioRxiv - Microbiology 2024Quote: HIV-1 RNA levels were measured in cell-free CSF and plasma at each site using the ultrasensitive Amplicor HIV-1 Monitor assay (versions 1.0 and 1.5; Roche Molecular Diagnostic Systems ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant was treated with 1 μg/mL DNase I (Roche) for 2 h at 37 °C before 0.22 μm filtration ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was then used for 10% SDS-PAGE and then transferred to a PVDF membrane (Roche). The PVDF membrane was then blocked with 5% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... BmE cells were transfected with 2 μg of this recombinant plasmid and A3 helper plasmid using X-tremeGENE HP DNA Transfection Reagent (Roche, Switzerland), and the culture medium was changed after 6 h ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were prehybridized in DIG Easy Hyb (#11796895001, Roche) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... DNA detection was performed using DIG High Prime DNA Labeling and Detection starter kit (Roche). The HR sequences were amplified from pSLI-SUN1-HAx3-glms and pSLI-SUN2-HAx3-glms ...
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bioRxiv - Microbiology 2024Quote: RNA extraction from saliva samples stored in RNAlater was performed with High Pure Viral RNA Kit (Roche), both according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Immunodetection was carried out by using mouse anti-GFP (1:1000, Roche), mouse anti-HA primary antibody (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-GFP (1:300, Roche), mouse anti-HA (1:100 Roche) ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-HA primary antibody (1:1000, Roche), rabbit anti-γH2A.X antibody (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... used at the indicated dilutions: mouse anti-GFP (Roche 1:300), mouse anti-HA (Roche:100) ...