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Citations for Roche :
3701 - 3750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Left lung lobes were dissociated by enzymatic digestion with 1 mg/mL collagenase D (Roche, 11088882001), and 1 mg/mL collagenase IV (Worthington Biochemical ...
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bioRxiv - Neuroscience 2024Quote: ... Protein visualization was performed by chemiluminescence using LumiLight western blotting (Roche) and ImageQuant 800 (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... and 100,000 worms were collected in 1 ml of RIPA buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... and three times with low salt buffer (20 mM Tris HCl pH 7.4, 10mM MgCl2, 0.2% Tween-20, Roche Protease Inhibitor and RNase Inhibitor). To elute RNA ...
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bioRxiv - Neuroscience 2024Quote: ... The agarose resin was washed once with high salt buffer (50 mM Tris HCl pH 7.4, 1M NaCl, 1 mM EDTA, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail and RNase Inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... EDTA-free complete mini–Protease Inhibitor Cocktails were from Roche (Indianapolis, IN). Pierce bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL 20 mg/mL Glycogen (Roche, 10901393001)) at 37°C for 2 hr and subsequently subjected to Proteinase K (15 μL 10% SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 x proteinase inhibitor cocktail (Roche, Germany). After centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (used only for CDKN1A ...
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bioRxiv - Molecular Biology 2024Quote: Tissue extracts were prepared by sonication in PBS containing 1x protease inhibitor cocktail (Roche). An aliquot was removed for protein determination by the Qubit assay ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each final pooled library was quantified by qPCR with the KAPA Library Quantification Kit (KAPA Biosystems, USA) and sequenced using the Illumina paired-end protocol on a HiSeq3000 sequencer by the Get-PlaGe core facility (GenoToul platform ...
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bioRxiv - Microbiology 2024Quote: ... As the DIG Easy Hyb (Roche) drastically reduces the background noise ...
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bioRxiv - Molecular Biology 2024Quote: ... containing cOmplete protease inhibitor cocktail (Roche, #4693116001). The tissue lysate was centrifuged at 16,000 x g for 20 min and supernatant was extracted for protein concentration measurement by Pierce BCA protein assay (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... using X-tremeGENE™ HP DNA Transfection Reagent (Roche). After 7 days at 28 °C the P1 virus stock was collected ...
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bioRxiv - Molecular Biology 2024Quote: For the purification of TFIIIC/3xFLAG-MYCN and 1A/3xFLAG-MYCN complexes Sf9 pellets were resuspended in a lysis buffer (25 mM Tris pH8, 200 mM NaCl, EDTA free cOmplete PI (Roche), 10 µg/ml DNAseI ...
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bioRxiv - Molecular Biology 2024Quote: ... It was performed in a LightCycler® 480 Instrument II thermocycler (Roche). For each reaction we used 4 μl of sample and 6 μl of a mixture with forward and reverse oligonucleotides and Sybr Green Premix.
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bioRxiv - Molecular Biology 2024Quote: Total RNA from dCasRx+gRNA transfected HEK293T cells was reverse transcribed using oligo dT and the Transcriptor First Strand cDNA Synthesis Kit (#04897030001, Roche) as manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... treated with Annexin-V-FLUOS (Roche Diagnostics) and analyzed by flow cytometer (Becton Dickinson).
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bioRxiv - Genetics 2024Quote: ... as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The libraries were multiplexed and clustered onto two flowcells and were loaded onto an Illumina HiSeq 4000 instrument ...
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bioRxiv - Neuroscience 2024Quote: ... phosphatase inhibitor cocktail (Roche), 1 μM phenylmethanesulfonyl fluoride ...
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bioRxiv - Molecular Biology 2024Quote: ... and EDTA-free proteinase inhibitor (Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted mRNA was then reverse transcribed using random hexamer primers and Transcriptor First Strand cDNA Synthesis Kit (#04897030001, Roche) at 65°C for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... lysed by 1 mg mL-1 Collagenase-D (Roche, Basel, Switzerland) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated in blocking solution (0.2% Roche block, 10% FBS, 1% DMSO in PBST) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... desoxycholic acid-Na-salt 1%, SDS 0.1%) (Serva electrophoresis, Germany) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Switzerland). Western blots were performed following routine protocols67 ...
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bioRxiv - Cancer Biology 2024Quote: ... and KAPA qPCR (Kapa Biosystems). The Illumina NovaSeq 6000 system was used to sequence libraries to a target read depth of 50,000 reads/cell ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.1% SDS) containing protease inhibitor cocktail (Complete; Roche) and phosphate inhibitors (10 mM NaF final concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... in buffer (10 mM Tris-Cl pH8.0, .25 mM EDTA, 100mM NaCl, 0.5% SDS 1mg/ml proteinase K) (Roche Cat #03508838103) at 55 °C for 4 hours to remove proteins ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Purified DNA was used for subsequent qPCR reactions using SYBR Green I Master Mix on a LightCycler 480 System (Roche). Enrichment was calculated using the following formula ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and random hexamer primers (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and PhosSTOP (Roche; 4906837001). Proteins were resolved using SDS-PAGE (Invitrogen NuPage 4-12%) ...
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bioRxiv - Cell Biology 2024Quote: ... at 2 mg/mL and DAPI (Roche) at 4 mg/mL into the pericardial sac ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche, Switzerland); pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... we applied the KAPA Library Quantification Kit (Kapa Biosystems) to ensure library quality for sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (3F10, Merck Millipore/Roche), and anti-α-Tubulin (B-5-1-2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × Complete EDTA-free protease inhibitor cocktail (Merck Millipore/Roche)] and sonicated with Bioruptor II (CosmoBio) ...
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bioRxiv - Microbiology 2024Quote: ... 3µl of the alkaline phosphatase-coupled anti-DIG antibody (Fab fragments, Roche, Cat No. 11093274910) were added to the buffer and incubation at room temperature on a belly-dancer was allowed for 30 more min ...
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bioRxiv - Microbiology 2024Quote: ... 1ml of CDP-Star® Chemiluminescent Substrate (Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate) (Roche, Cat No. 11685627001) was added to 9ml of DIG-detection buffer and membranes were then incubated with the substrate for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Dnase I was obtained from Roche (11284932001). Neurobasal medium was obtained from Gibco (21103049) ...
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bioRxiv - Molecular Biology 2024Quote: ... protease inhibitor cocktail (Roche Complete protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... protease inhibitor cocktail (Roche, Complete protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... and the KAPA Library Quantification Kit (Roche) before proceeding with sequencing using NovaSeq 6000 (Illumina).
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bioRxiv - Microbiology 2024Quote: ... 2 mM EDTA) supplemented with 1X cOmplete EDTA-free protease inhibitor cocktail (Roche), 2 mM MgCl2 ...
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bioRxiv - Microbiology 2024Quote: ... and membranes were finally incubated with bioluminescent reagents (BM Chemiluminescence Western Blotting Kit, Roche) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... or the Sensitive RT HS-PCR Mix (2017-2000, AA Biotech, Gdansk, Poland) on the LightCycler480 (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM EDTA and cOmplete EDTA-free protease inhibitor cocktail (Roche). After low-speed centrifugation at 1,000 ×g and 3,000 ×g ...
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bioRxiv - Biochemistry 2024Quote: ... and protease inhibitor cocktail (Roche). The crude membrane fraction was derived from sonication and ultracentrifugation (sonication method) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% Triton X-100 and 1 EDTA-free protease inhibitor cocktail tablet (Roche: Cat#05056489001) per 50 mL buffer ...
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bioRxiv - Bioengineering 2024Quote: ... cOmpleteTM EDTA-free protease inhibitor cocktail was from Roche (Switzerland). Acrylamide bis-acrylamide 30% solution ...