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Citations for Roche :
4051 - 4100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... supplemented with a protease-inhibitor cocktail (Roche)) at a concentration of 8 × 106 cells/mL and equilibrated on ice for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... A probe binding the left flank of the acbd4/5 locus was generated using DIG probe labelling mix (Roche) as per the manufacturer’s instruction (primers acbd4/5_LF_fw and acbd4/5_LF_rv ...
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bioRxiv - Developmental Biology 2024Quote: ... tris(hydroxymethyl)aminomethane (Tris hydrochloride, Roche), and sodium chloride (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... the libraries were quantified by qPCR using the KAPA library quantification kit for Illumina platforms (Roche, KR0405) using QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... in nuclear lysis buffer (50 mM Tris-HCl [pH 7.5], 10 mM NaCl, 0.2% NP-40, 1x Roche protease inhibitors) at 4
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bioRxiv - Genomics 2024Quote: ... The post-enrichment PCR amplification was performed using KAPA HiFi HotStart ReadyMix (Roche) and IS5 and IS6 primers as described above ...
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bioRxiv - Genomics 2024Quote: ... 1× protease inhibitor (Roche, 11873580001), RNase inhibitor (0.4 U/µl ...
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bioRxiv - Genomics 2024Quote: ... The 10 million of crosslinked cells were resuspended in ice-cold 1.5 ml of Hi-C lysis buffer (10mM Tris-HCl pH 8.0, 10mM NaCl, 0.2% NP-40) supplemented with 1x protease inhibitors (PI; Roche, 04693116001), incubated on ice for 20 min and centrifuged at 1200 rpm ...
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bioRxiv - Genomics 2024Quote: ... The concentration of the single-cell library was accurately determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the paired-end sequencing using NovaSeq 6000 platforms (Illumina) ...
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bioRxiv - Genomics 2024Quote: ... 1× protease inhibitor (Roche, 11873580001), RNase inhibitor (0.4 U/µl ...
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bioRxiv - Genomics 2024Quote: ... followed by a cell lysis with Hi-C lysis buffer (10 mM Tris-HCl [pH 8.0], 10 mM NaCl, 0.2% NP-40, 1x Roche protease inhibitors) for 30 minutes at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... Libraries were quantified by qPCR (KAPA Biosystems) and sequenced with 151 bp paired end reads on an Illumina NextSeq instrument ...
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bioRxiv - Genomics 2024Quote: ... followed by gap repair with Kapa HiFi HotStart Uracil+ DNA Polymerase (KAPA Biosystems) and Taq DNA ligase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... PCR step 2 was conducted using 10-15ng PCR1 product to complete Illumina sequencing adaptors (P5-dual-index. F and P7-dual-index. R) using Kapa HF Hot Start Ready Mix (Roche, KK2602) with following PCR conditions denaturation - 98°C for 0:45 ...
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bioRxiv - Genomics 2024Quote: ... using Kapa HiFi Polymerase (KAPA Biosystems). Libraries were quantified by qPCR (KAPA Biosystems ...
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bioRxiv - Genomics 2024Quote: Library preparation was carried out at the University of Michigan Advanced Genomics Core using the KAPA mRNA Hyper Prep Kit (Roche, Wilmington, MA, USA) with dual indexing adapters ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... then were digested for 20 min at 37 °C with Liberase TM (Roche) plus DNase I (Roche) ...
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bioRxiv - Immunology 2024Quote: ... plus DNase I (Roche). Tissue pieces were strained through 40μm cell strainer into single cells followed by treatment with ACK Solution (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of ddH2O (Roche, Basel, Switzerland) and 0.5 µL (80 ng/µL ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.50 μl 10x PCR reaction buffer including 15 mM MgCl2 (Roche); 1.50 μl dNTPs mix (250 μM each dNTP) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 U of enzyme mix using the Expand Long Template PCR system (Roche). Samples were amplified with the following thermocycler conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were carried out in 15 µl using the Taq DNA Polymerase kit (Roche) and the following conditions ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed in RIPA buffer supplemented with 1x EDTA-free Protease Inhibitor cocktail (Roche), 1mM PMSF and 35 nM β-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells intended for sucrose sedimentation were disrupted in standard lysis buffer with 1X protease inhibitor cocktail replaced by 1x complete mini EDTA-free protease inhibitor cocktail (Roche #04693159001). 1X Phosphatase Inhibitor (Sigma-Aldrich #P5726 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems). PCR products were cloned in pGEM-T Easy Vector (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1x complete mini EDTA-free protease inhibitor cocktail (Roche #04693159001). Cell extracts were clarified by centrifugation and were sedimented by ultracentrifugation for 120 min at 70,000 rpm (Beckman Optima Max-TL ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were washed three times with 10 ml of ice-cold PBS supplemented with Complete Protease Inhibitor (Roche, Basel, Switzerland), and the cells were resuspended in 1 ml of the same buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... CP values were calculated using the Second Derivative Maximum method implemented in the LightCycler 480 Software (Roche). CT values were calculated using the Comparative CT (ΔΔCT ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 1x Protease inhibitor (Roche # 11873580001) and incubated at 4°C for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were proteolyzed with sequencing grade trypsin (Roche) using 0.01% Protease-MAX surfactant (Promega).
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bioRxiv - Immunology 2024Quote: ... and 125 ug/ml of DNAse I (Roche 11284932001) for 30’ and then mechanically disaggregated in gentleMACS® tissue dissociator.
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bioRxiv - Immunology 2024Quote: ... with DNAse I 0,1mg/ml (Roche 11-284-932-001) and Liberase® 0,1 mg/ml (Roche 05-401-020-001).
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bioRxiv - Immunology 2024Quote: ... sciatic nerves and brains were crushed with the back of a 3ml syringe in a serrated 24 well plate and triturated in digestion media (Liberase TM (0.208 WU/ml) (Roche Cat # 054010200001) and DNase I (40 ug/ml ...
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bioRxiv - Immunology 2024Quote: Proteins were extracted from tissues or cells in radioimmunoprecipitation assay (RIPA) buffer (Beyotime) containing a complete protease inhibitor cocktail (Roche), resolved by SDSPAGE ...
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bioRxiv - Immunology 2024Quote: ... with 0.17 U/ml of Liberase® (Roche 05-401-020-001), 1 mg/ml of collagenase (Sigma-Aldrich 11088866001 ...
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bioRxiv - Immunology 2024Quote: ... 5% glycerol and 1x EDTA-free protease inhibitor cocktail (PIC) (Roche).
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bioRxiv - Immunology 2024Quote: ... Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche). Sequencing was carried out on the NovaSeq 6000 instrument from Illumina using paired-end 2 × 100 bp ...
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bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
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bioRxiv - Immunology 2024Quote: ... and 0.2 mg/mL DNaseI (Roche, 11284932001) for 30 minutes at 37°C while shaking at 180 rpm ...
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bioRxiv - Immunology 2024Quote: ... and 0.4 mg/mL DNaseI (Roche, 11284932001), for 30 minutes at 37 °C with shaking (180 rpm) ...
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bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Immunology 2024Quote: ... and then i5/i7 barcoded primers were added to tagmented cDNA using Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (Roche) to barcode amplicons ...
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bioRxiv - Immunology 2024Quote: ... The thymic pieces were then transferred to a digestion solution containing collagenase/dispose (Roche) at 1mg/ml and DNase I 20ng/ ml in 8ml of 2% FBS + PRMI1640 medium ...
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bioRxiv - Microbiology 2024Quote: ... 500mL of Buffer 1 (20mM KHEPES pH 7.9, 50mM KCl, 10% Glycerol) with a protease inhibitor tablet (Roche) was added and samples were subject to ultrasonication in a cup horn sonifier (Branson ...
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bioRxiv - Microbiology 2024Quote: ... 1 mg/mL complete mini EDTA-free protease inhibitor (Roche), and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in cold Buffer A-5 (20mM Tris-HCl, pH 7.9, 500mM NaCl, 5mM Imidazole) with an EDTA-free protease inhibitor tablet (Roche) and kept on ice ...
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bioRxiv - Immunology 2024Quote: ... Mouse lungs were excised and lightly homogenized in HEPES buffer containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNaseI (30 μg/ml ...