Labshake search
Citations for Roche :
3501 - 3550 of 5002 citations for NMB SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ZIK1 sense and antisense RNA probes labeled with digoxigenin were synthesized using the DIG Northern Starter Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA libraries for all samples were prepared using a KAPA Hyper Prep kit and PCR-free protocol (Roche). For each genotype ...
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bioRxiv - Cell Biology 2023Quote: ... and 100 / 30 ng (immunoprecipitation/input) used for library preparation using KAPA LTP library preparation kit (KAPA Biosystems). Library preparation was stopped prior to PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... hybridization capture was performed using the SHS method according to the SeqCap EZ Reagent Kit Plus (Roche NimbleGen) with modifications to the number of hybridization captures and the number of post-capture ligation-mediated PCR (LM-PCR ...
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bioRxiv - Plant Biology 2023Quote: ... transferred on nylon membrane and hybridized with DIG-labelled probes obtained by PCR DIG Probe Synthesis Kit (Roche) with specific primers (see table primers list) ...
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bioRxiv - Microbiology 2024Quote: ... Draining lymph nodes were harvested and preserved in a lysis solution from High Pure DNA Isolation Kit (Roche).
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bioRxiv - Developmental Biology 2024Quote: The RNA probes for detected genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Germany). ISH was performed on 5 μm 4% paraformaldehyde-fixed paraffin-embedded sections from the testicular tissues ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Cell Biology 2024Quote: Libraries were constructed on 150 ng of input DNA with the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Pooled libraries were paired-end sequenced on a High Throughput Run Mode flow cell with the V4 sequencing chemistry on an Illumina HiSeq 2500 platform (Illumina ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Genomics 2024Quote: ... we constructed PE and SE libraries using the KAPA Hyper Library Preparation Kit (Kapa Biosystems, Woburn, MA, USA) and the Accel NGS 1S plus DNA kit (Swift BioSciences ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
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bioRxiv - Molecular Biology 2024Quote: The peptidyl-tRNAs in the PTS buffer were isolated by using a High Pure miRNA Isolation Kit (Roche), as described previously (54) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared uniquely dual-indexed libraries for Illumina sequencing using the KAPA HyperPrep Kit (Roche, Indianapolis, Indiana, USA) using one-quarter reagent volumes ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from mouse tail clippings and PCR amplified using the KAPA Mouse Genotyping Kit (Roche) following manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... The total RNAs were converted into individually barcoded polyadenylated mRNAseq libraries with the Kapa HyperPrep mRNA kit (Roche). Libraries were barcoded with Unique Dual Indexes (UDI’s ...
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bioRxiv - Systems Biology 2024Quote: Paraffin-embedded tissue sections were processed with an in-situ cell-death detection kit (11684795910; Roche, Basel, Switzerland) for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... Pax3 digoxigenin (DIG) - labeled antisense riboprobes were transcribed from linearized gene-specific probes (PCR DIG probe Synthesis Kit, Roche). WISH experiment was performed as follows ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were generated using the KAPA HyperPrep Kit with RiboErase (HMR) and KAPA dual-indexed adapters (Roche Sequencing and Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted by combining phenol-chloroform–isopropanol treatment using the High Pure RNA Isolation Kit (Roche, Germany) (101) ...
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bioRxiv - Genomics 2020Quote: ... The sheared DNA was used for paired-end library preparation with a KAPA LTP Library Preparation Kit (KAPA Biosystems). The optimal numbers of PCR cycles for individual libraries were determined with a Real-Time Library Amplification Kit (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems; Cat#KK4835). Libraries were then pooled and sequenced (1×75bp ...
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bioRxiv - Neuroscience 2019Quote: ... The terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay was performed using the TMR red kit from Roche Diagnostics (Mannheim ...
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bioRxiv - Genomics 2019Quote: We constructed dual indexed libraries using 100-250ng of genomic DNA and the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: One microgram of total RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with the oligonucleotide (dT)18 primer according to the manufacturer’s instructions (30,34) ...
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bioRxiv - Physiology 2019Quote: ... Free fatty acid and triglyceride levels were measured in serum using the colorimetric quantification kits Half-micro test (Roche) and Infinity (ThermoScientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed by the Dana-Farber Molecular Biology Core Facility using the KAPA mRNA HyperPrep Kit (Roche). Nucleic acid was sequenced using an Illumina NextSeq 500 instrument (PE75 kit).
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bioRxiv - Microbiology 2019Quote: ... and qPCR based library quantification was performed using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems, KK4824) on a LightCycler480 (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: ... To generate the dsRNAs for the rescreen cDNA was generated using the Transcriptor First Strand cDNA Synthesis Kit (Roche). The cDNA was then used to amplify fragments by PCR with the same primers as used by Eupheria Biotec GmbH (Dresden ...
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bioRxiv - Genetics 2020Quote: ... Staining for apoptotic cells was performed using the AP-In situ Cell Death Detection Kit (Roche Diagnostics, Penzberg, Germany) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... The digoxigenin-labeled RNA probes were prepared using a DIG RNA labeling kit according to the manufacturer’s protocol (Roche) using each cDNA clone as the template ...
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bioRxiv - Neuroscience 2019Quote: ... Antisense and sense digoxigenin (DIG)-labelled RNA probes were generated using the DIG RNA labelling kit (Roche Diagnostics, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 40 ng of DNA per sample was subjected to qPCR analysis using LightCycler 480 Probe Master-Mix kit (Roche) and primer sets for γHV68 gB and host NFAT5 (Table S3) ...
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bioRxiv - Genomics 2019Quote: K111 insertions were amplified by PCR using the Expand Long Range dNTPack PCR kit (Roche Applied Science, Indianapolis, IN) as described ...
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bioRxiv - Neuroscience 2019Quote: ... barcoded and enriched using the NimbleGen SeqCap EZ Human Exome Library v2.0 enrichment kit (Roche NimbleGen, Madison, WI, USA). Purified and quantified library pool was subsequently sequenced on an Illumina HiSeq 2000 sequencing instrument (Illumina ...
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bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR was performed with the LIght Cycler DNA Fast Start Master SYBR green I kit (Roche) in a Light Cycler 480 Instrument II at Tm=60°C ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-sequencing libraries were then prepared either manually with the KAPA Stranded mRNA-seq library prep kit (KAPA Biosystems), or with automation ...
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bioRxiv - Genomics 2019Quote: ... Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal) (Kapa Biosystems, Woburn, MA). The libraries were pooled and sequenced on a 100 bp paired-end Illumina HiSeq 2500 or 4000 run (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Total RNA sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase for Illumina Platforms (KAPA Biosystems). Briefly ...
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bioRxiv - Genomics 2021Quote: ... RNA-sequencing (RNA-seq) libraries were generated from 300ng RNA using the KAPA RNA HyperPrep Kit with RiboErase (Roche) and sequenced with NovaSeq 6000 (2×150 bp ...
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bioRxiv - Cell Biology 2021Quote: ... A cDNA library was made from Oligo(dT)12–18 as described in the manufacturer’s protocol (Transcriptor First Strand cDNA Synthesis Kit, Roche). Quantitative PCR was performed using Quantifast SYBR Green PCR Kit (204052 ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using 50 ng of DNA with the KAPA HyperPlus Kit as per the manufacturer’s protocol (Roche). The libraries were sequenced with a paired-end read length of 150bp ...
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bioRxiv - Microbiology 2021Quote: ... The final pool was quantified by qPCR with Kapa Library Quant Kit for Illumina Platforms (KapaBiosystems, Roche, Cat. # KK4824) on an AriaMX Real Time PCR System (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from clinical isolates using the AMPLICOR® Respiratory Specimen Preparation Kit (Roche, Basel, Switzerland) and purified with 1.8X AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Samples were further processed using the Kapa LTP Library Preparation Kit for Illumina Platforms (Kapa Biosystems, Part Number KK8232) and indexed with the NEBNext Multiplex Oligos for Illumina 96 Unique Dual Index Primer Pairs (New England Biolabs ...