Labshake search
Citations for Roche :
3301 - 3350 of 5002 citations for NMB SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Clinical Trials 2019Quote: ... The pooled library was created with 50 ng DNA per sample and quantified using Kapa Kit (Kapa Biosystems). The pooled library was run on Illumina-MiSeq with 15% PhiX mock library to reduce discrepancies in read clustering ...
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bioRxiv - Neuroscience 2019Quote: ... Library quantification and quality checks were done using KAPA Library Quantification Kit for Illumina (KAPA Biosystems, Wilmington, MA), High Sensitivity DNA Kit on BioAnalyzer (Agilent ...
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bioRxiv - Genomics 2021Quote: ... Each entire ChIP sample was used to prepare Illumina sequencing libraries using the KAPA Hyper Prep Kit (Roche). Adapter-ligated DNA was separated on a 2% E-Gel EX (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... All libraries were quantified on the Agilent 2100 hsDNA BioAnalyzer and KAPA library quantification kit (Roche Cat# 07960140001). The resulting gene expression libraries were sequenced on an Illumina sequencing platform with a 28 × 8 × 60 bp configuration at a targeted depth of 25000 reads per cell ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 100 ng of total RNA was processed using reverse transcription kit to synthesize cDNA (Roche, Basel, Switzerland). The primer sequences used for RT-PCR analysis are listed in Table S1 ...
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bioRxiv - Microbiology 2021Quote: ... RNA libraries were quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems), pooled in equimolar quantities and single-end sequenced on a HiSeq 2500 platform ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR were performed using the LightCycler 480 SYBR Green I Master Kit on a LightCycler480 apparatus (Roche) using standard protocols (40 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... For CHIP-qPCR: qPCR was performed on diluted samples using LightCycler 480 SYBR green 1 master kit (Roche) using primers listed below.
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bioRxiv - Plant Biology 2021Quote: Genomic DNA (5 μg) was used to construct the BS-seq library with the KAPA Library kit (Roche) and EpiTect Bisulfite Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 350 µL of saliva were mixed with 350µL of lysis buffer (MagNA Pure Compact RNA Isolation Kit, Roche). RNA was extracted from 500 μl of sample using a MagNA Pure Compact Instrument (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... TruSeq-style library preparation was carried out using either the KAPA Hyper Prep kit (Kapa Biosystems, Wilmington, MA) or the NEB DNA Ultra 2 kit following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Preparation of DNA sample library was done with the use of Kapa HTP Library Preparation Kit (Roche, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: RNA of high quality (RINe>8) was prepared for mRNA-sequencing using the KAPA mRNA HyperPrep kit (Roche). Libraries were quantified using a High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed following the manufacturer’s instructions with the Roche FastStart Universal SYBR Green Master Kit (Roche). The same primers reported above for tp0574 and tprL (but without restriction tags in the latter case ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA was reverse transcribed using ‘Transcriptor High Fidelity cDNA Synthesis Kit’ (Roche Diagnostics, Mannheim, Germany) with oligodT as a primer ...
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bioRxiv - Neuroscience 2020Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany). Biotin-labeled probes were synthesized by substitution of DIG RNA labeling mix with biotin RNA labeling mix (Roche ...
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bioRxiv - Microbiology 2021Quote: ... 500 ng RNA was reverse transcribed using random hexamers and the Transcriptor First Strand cDNA Synthesis Kit (Roche) following the protocol recommended by the manufacturer ...
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bioRxiv - Genomics 2020Quote: Southern blots were performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and the DIG Wash and Block Buffer Set (Roche 11585762001) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon pool was normalized using quantification by qPCR with the KAPA Illumina quantification kit (Roche, Basel, Switzerland). The computational processing of these data was performed using QIIME2 2017.11 (Bolyen et al. ...
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bioRxiv - Genomics 2021Quote: ... The libraries were individually quantified via qPCR using a KAPA Library Quantification Kits (Kapa Biosystems, Wilmington, MA, USA) for Illumina platforms ...
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bioRxiv - Cell Biology 2020Quote: ... followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
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bioRxiv - Microbiology 2021Quote: ... DNA insertion fragments and backbones were amplified using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Boston, USA), and they were assembled at 50 °C for 1 h.
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bioRxiv - Physiology 2021Quote: ... RNA libraries were prepared using Kapa Stranded RNA-seq kits on samples with a RIN>0.8 according to the manufacturer’s instructions (Roche). Library quantities were determined using QUBIT (ThermoFisher ...
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bioRxiv - Systems Biology 2020Quote: ... We ran 10 μl reactions using the LightCycler 480 platform and the SYBR Green I Master kit (Roche Diagnostics Corporation ...
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bioRxiv - Cancer Biology 2021Quote: The TUNEL staining was performed using an In Situ Cell Death Detection Kit (Roche Diagnostics GmbH, Mannheim, Germany). The paraffin sections were stained with TUNEL reaction mixture for 1 h at 37°C and then mounted using DAPI containing mount medium ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems, K4602) using specific primers for β-actin ...
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bioRxiv - Cancer Biology 2021Quote: RNA-seq libraries for the COH TNBC cohort were generated using the KAPA Stranded mRNA-Seq kit (Roche) according to manufacturer’s instructions and were sequenced on a HiSeq4000 Illumina platform to generate 75 bp paired end reads ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Specific DNA probes for hpt and OsSOG1 were synthesized with a PCR Digoxigenin Probe Synthesis Kit (Roche Diagnostics) using the primers shown in Table S7.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA from cell culture and mouse heart tissue was extracted using high pure RNA isolation kit (Roche, 11828665001) and miRNeasy Mini kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The adapter-ligated fragments were quantified by qPCR using the Library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The adapter-ligated fragments were quantified by qPCR using the Library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad) ...
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bioRxiv - Genomics 2022Quote: ... Cycle Pure Kit (Omega) and then labelled with either biotin–16-dUTP or digoxigenin–11-dUTP (Roche Diagnostics) using the BioPrime Array CGH random priming kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... and immune complexes were visualized using the ultraView Universal DAB (diaminobenzidine tetrahydrochloride) Detection Kit (Roche cat#760-500). Slides were then washed with a Tris-based-reaction buffer (Roche cat#950-300 ...
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bioRxiv - Microbiology 2022Quote: ... and vRNA were generated by reverse-transcription PCR (RT-PCR) using Transcriptor First Strand cDNA Synthesis Kit (Roche). Primers with specific tags for differentiations of virus mRNA ...
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bioRxiv - Genetics 2022Quote: ... Quantitative real-time PCR (qPCR) was carried out using a KAPA™ SYBR FAST qPCR Kit (KAPA Biosystems) and StepOnePlus™ Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: Southern blot was performed according to DIG High Prime DNA Labelling and Detection Starter Kit II (11585614910, Roche). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Afterwards samples were incubated for 2 h in the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) enzyme labeling solution at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The single strand DNA oligo was first labelled with Digoxigenin at 5’-end using end labelling kit (Roche) as per the manufacturers’ protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Secreted PTH was measured by electro-chemical luminescence immuno assay (ECLIA) using an Elecsys PTH kit (Roche, Germany).
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bioRxiv - Neuroscience 2019Quote: ... Library size produced was analysed using qPCR using the Library Quantitation kit/Illumina GA /ABI Prism (Kapa Biosystems). Libraries were barcoded ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The samples were centrifuged and the TC content in the supernatant was measured using commercial kits (Roche Diagnostics) with the Cobas c501 autoanalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-radioactive antisense RNA probes were generated by in vitro transcription using DIG RNA labeling kit (Roche Diagnostics). For histological analysis sections were stained with Hematoxylin and Eosin according standard protocols.
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was used as a template for quantitative PCR analysis using the SybrGREEN® Master Kit (Roche) and a Light Cycler 1.5® (Roche) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR was conducted using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (KAPA Biosystems, MA, USA) and LightCycler 96 (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... Secreted HBV DNA was extracted according to manufacturer provided protocol (Realtime Ready Cell Lysis Kit, Roche, Mannheim, Germany) and quantified in a qPCR assay (LightCycler® 480 SYBR Green I Master ...
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bioRxiv - Genomics 2019Quote: ... PCR optimisation was carried out on the full-length cDNA using the KAPA HiFi PCR kit (Kapa Biosystems) and 10—12 cycles was sufficient to generate the material required for SMRTbell library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... Barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA), and sequencing performed on a NovaSeq 6000 (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Probe-target hybrids were visualized by a chemiluminescent assay with the DIG luminescent detection kit (Roche Molecular Biochemicals), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: RNA was isolated from the neural cultures and VeroE6 cells using the High Pure RNA Isolation Kit (Roche). The concentration of RNA was determined using Nanodrop ...