Labshake search
Citations for Roche :
3451 - 3500 of 5002 citations for NMB SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Probes were transcribed with TranscriptAid T7 High Yield Transcription kit (Thermo, KO441) using DIG-Label UTP (Roche,11127073910).
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bioRxiv - Molecular Biology 2024Quote: ... Library amplification was performed following the KAPA mRNA Hyper Prep Kit Technical Data Sheet (KR1352 – v4.17, Roche Corporate). Each resulting indexed library was quantified ...
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bioRxiv - Genomics 2024Quote: ... they were purified in large volume columns using the High Pure Viral Nucleic Acid Large Volume Kit (Roche) with 2.5 mL of PB buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was amplified using the 2X KAPA Taq ReadyMix PCR Kit (KAPA Biosystems, now Roche Sequencing, Indianapolis, IN) for 35 cycles using an annealing temperature of 64°C ...
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bioRxiv - Molecular Biology 2024Quote: Labelling of probes with digoxigenin was done using the 2nd generation DIG Gel Shift EMSA kit (Roche, USA) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. No. 0796014001.) Gene expression libraries were sequenced at a targeted depth of 25,000 reads per cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Library quantification was conducted using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Fragment average size was determined using the LabChip GXII instrument (PerkinElmer) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were prepared from 1 µg of total RNA using a Kapa mRNA HyperPrep kit (Roche, KK8580) per the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... Full-length RNA was made into libraries using the KAPA stranded mRNA library prep kit (Roche, Nutley, NJ) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Lactate was quantified using a lactate assay kit (D-Lactic/L-Lactic acid UV method, r-biopharm, Roche). All samples were measured using fresh growth medium as control.
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bioRxiv - Biochemistry 2024Quote: Cell metabolic viability was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Roche). After treatments ...
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bioRxiv - Developmental Biology 2023Quote: ... Full length cDNA was then converted into an Illumina sequencing library using the Kapa Hyper Prep kit (Roche). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription of Digoxigenin/Fluorescent-labeled probes was performed using an RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche) using 250 ng of total RNAs as input ...
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bioRxiv - Neuroscience 2023Quote: ... remaining tissue was lysed and gDNA amplified by means of PCR (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) as described above ...
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bioRxiv - Genomics 2023Quote: ... The end-repair and A-tailing (ERAT) enzyme mix with a purple cap (KAPA HyperPlus kit, Roche v5.19) needs to be used for this protocol since the ERAT enzyme mix with an orange cap in the KAPA HyperPlus kit ...
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bioRxiv - Neuroscience 2023Quote: ... Salivary cortisol concentrations were measured with electro-chemiluminescence-assay (ECLIA) kit (Cobas®, Roche Diagnostics GmbH, Mannheim, Germany). Samples (IDs ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from tumoroids was done following the manufacturer protocol using the High Pure RNA Isolation Kit (Roche).
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bioRxiv - Evolutionary Biology 2022Quote: ... A total of 100 ng RNA was subsequently reverse transcribed using the Transcriptor First Strand cDNASynthesis Kit (Roche) at 55 °C for cDNA synthesis ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland). In situ hybridizations were performed on 16μm cryosections that were rehydrated in 1x PBS for 5min ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were stained using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL apoptosis assay kit, Roche, American), and the cells were viewed under a fluorescence microscope.
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bioRxiv - Microbiology 2023Quote: ... RNA was re-extracted from original material using the High Pure RNA Isolation Kit (Roche Diagnostics GmbH Mannheim). A multi segment RT-PCR amplification was performed using the Superscript III high-fidelity RT-PCR Kit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, cat#KK4824) using 7900HT Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... 500 ng of RNA was prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative PCR (RT-qPCR) with the SensiFAST SYBR No-ROX One-Step Kit in a LightCycler 96 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ul of each sample were used for library quantification using the KAPA Library Quantification Kit (Roche #KK4923). The rest of the DNA eluate (20ul ...
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bioRxiv - Plant Biology 2023Quote: ... Hybridization and probe detection was performed using a DIG Luminescent Detection Kit for Nucleic Acids (Roche Diagnostics, UK) as per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: Low-pass WGS libraries were created using the Kapa Biosystems Hyper Prep kit (KAPA Biosystems, Capetown, South Africa) using 11.8 ng/ml of G4_1 ...
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bioRxiv - Cancer Biology 2023Quote: Proliferation was measured by using 5-bromo-2’-deoxy-uridine (BrdU) labelling and detection kit (Roche, BSL, CH) as described earlier (25) ...
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bioRxiv - Neuroscience 2023Quote: ... The assay was performed using the In Situ Cell Death Detection (TUNEL) Kit with TMR Red (Roche, 12156792910) as previously described13 ...
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bioRxiv - Microbiology 2023Quote: The RNA of all cell culture supernatants was extracted using High Pure Viral RNA kit (Roche, REF.11858882001). Viral RNA quantification in each sample was performed by quantitative reverse transcription PCR (RT-qPCR ...
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bioRxiv - Genomics 2023Quote: ... We quantified libraries by qPCR using the Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, cat#KK4824) and the 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The optimal numbers of PCR cycles were pre-determined using the KAPA Real-Time Library Amplification Kit (Roche) as described previously (Tanegashima et al ...
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bioRxiv - Immunology 2023Quote: ... 100 ng total RNA was reverse transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labeled cRNA probes were prepared via in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland) from the Prokr2 template available from Genepaint (https://gp3.mpg.de/viewer/setInfo/MH1790/12) ...
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bioRxiv - Cancer Biology 2022Quote: ... or PPIA) was performed in duplicates with fast start universal SYBR Green master (ROX) PCR kit (Roche, 04913914001) using QuantStudio7 (Thermofisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Both probes were labeled with fluorescein or digoxigenin (DIG) using RNA labeling kit (Roche Applied Science, Mannheim, Germany). The heads of fish at 4 mpf were fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized with Anchored-oligo (dT)18 primers and the Transcriptor First Strand cDNA Synthesis Kit (Roche). cDNA was diluted five-fold with water and 2-4μl of the diluted cDNA served as template for each qRT-PCR as described previously 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... The library pool was quantified by qPCR using a Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems). Paired-end 75bp sequencing was carried out by clustering 1.5pM of the library pool on a NextSeq 500 sequencer (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... Libraries preparation were performed with a Kapa Hyper Prep Kits with KAPA Library Amplification Primer Mix (Roche, 07962347001), quantified by Qubit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Physiology 2023Quote: ... the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) in situ cell death detection kit (Roche, Basel, Switzerland) was used according to the manufacturer’s instructions (including incubation for 1 h at 37 °C) ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed with 1 μg of purified RNA using Transcriptor First Strand cDNA synthesis KIT (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Sequencing library preparation and probe capture enrichment were performed according to the SeqCap EZ Developer Kit (Roche NimbleGen) or the SeqCap RNA Developer Enrichment Kit (Roche NimbleGen) ...
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bioRxiv - Neuroscience 2023Quote: TUNEL staining was performed for paraffin sections using the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... LDH was measured using the Cytotoxicity Detection Kit according to the manufacturer’s instructions (ref: 11644793001, Roche, Basel, Switzerland) and absorbance (ABS ...
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bioRxiv - Genetics 2023Quote: ... mRNA-seq libraries were constructed from 600ng of total RNA using the KAPA mRNA HyperPrep Kit (Kapa Biosystems). Three independent biological replicates were analyzed for each genotype ...
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bioRxiv - Microbiology 2023Quote: ... Upon reaching OD600= 0.3 total RNA was extracted from cells using a High Pure RNA Isolation Kit (Roche). Samples at different time points were centrifuged for 5 min at 2,000 relative centrifugal force and pellets were flash-frozen in liquid nitrogen ...
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bioRxiv - Genomics 2023Quote: ... A digoxigenin labeled probe was generated by PCR following the manufacturer’s instructions (PCR DIG Probe Synthesis Kit,Roche). The 993 bp probe (Cd709 chr1 ...