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Citations for Roche :
3451 - 3500 of 3510 citations for 4 Chloro 2 iodothieno 2 3 b pyridine 5 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were made using HEK-293/17 cells that were transfected with 5 μg of viral DNA using X-tremeGENE HP (Roche, Basel, Switzerland) after 48 h incubation ...
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bioRxiv - Cell Biology 2022Quote: ... in 5% non-fat dry milk in TBS/0.1% Tween-20 (TBST) or in 1% Western blocking reagent (WBR) (Roche Applied Science, Germany) in TBS (for β-actin) ...
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bioRxiv - Cell Biology 2023Quote: The cell pellets were washed once in PBS and resuspended in 5 ml PBS containing protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were brought to room temperature and washed twice in TNT before being incubated in blocking buffer (TNT+5% sheep serum+1% Roche blocking reagent) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The microinjection was performed with glass capillaries (Eppendrof Femtotips, Cat# 930000035) loaded with 5 mg/mL of RNase A (Roche, Cat# 10109142001) mixed with Dextran-TAMRA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 fly heads were homogenised in guanidine extraction buffer (5M Guanidinium-HClSigma G3272, 50 mM Hepes Sigma H3375, 5 mM EDTA, protease inhibitor cocktail Roche Complete Mini). The plate was coated with first antibody solution (Clone 6E10 ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkaline phosphatase staining was conducted on primary OP6 cell cultures on Day-5 post-transfection using NBT/BCIP stock solution (Roche/Sigma 11681451001) or BCIP/NBT-Blue Liquid Substrate Systems for Membranes (Sigma B3804 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Crosslinked cell pellets were lysed in 5 mL cold lysis buffer (10 mM Tris-HCl, pH7.5; 10 mM NaCl; 5 mM MgCl; 0.1 mM EGTA; 1x Roche Complete protease inhibitor cocktail). Lysis was allowed to proceed for minutes on ice with intermittent gentle pipetting to obtain homogeneous suspensions of nuclei ...
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bioRxiv - Microbiology 2024Quote: ... Samples were lysed in 1% Triton X-100 (20 mM Tris [pH 7], 150 mM NaCl, 5 mM MgCl2) containing protease inhibitor (Roche, Cat# 11697498001) with end-over-end rotation for 20 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Chloroplasts were washed with isolation buffer and collected for further analyses or lysed with osmotic lysis buffer (20 mM HEPES-KOH pH 8.0, 5 mM MgCl2, tablet of Roche Protease Inhibitor Cocktail). After centrifugation 15000 x g for 15 min ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were blocked in 5% (w/v) nonfat dry milk and probed with anti-HA antibodies (Roche 11867431001; 1:10,000 dilution), followed by horseradish peroxidase-coupled secondary anti-rat antibody (Sigma-Aldrich A9037 ...
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bioRxiv - Biochemistry 2024Quote: ... and the pellet was resuspended in 5 volumes of lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland); buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in 5% milk in PBS with 0.001% Tween-20 (PBST) before incubation with the specified antibodies prepared in 5% milk (GFP (Roche, 11814460001, 1:1,000), FLAG-HRP (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... HCT116 cells were lysed in salt-free lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, and one Roche complete protease inhibitor tablet). For the endogenous immunoprecipitation (IP) ...
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bioRxiv - Cell Biology 2024Quote: ... lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, and one Roche complete protease inhibitor tablet). Samples were centrifuged at 15,000 g for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, and one Roche complete protease inhibitor tablet)25,27,35 ...
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bioRxiv - Cell Biology 2024Quote: ... lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, and one Roche complete protease inhibitor tablet)25,27,35 ...
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bioRxiv - Cell Biology 2024Quote: ... lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, and one Roche complete protease inhibitor tablet)25,27,35 ...
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bioRxiv - Immunology 2020Quote: ... and a 30-gauge needle was used to inflate each pancreas through the common bile duct with 4 mL of media supplemented with 0.8 mg/mL of collagenase P (Roche, cat. number 11-249-002-001) and filtered at 0.22 μm prior to injection ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5% SDS, 0.5% SDO, 1% Triton X-100, 1 mM PMSF, Roche cOmplete Mini Protease Inhibitors 1x), and homogenized (Precellys Evolution ...
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membrane was blocked with 5% skim milk in TBS for 1 h and incubated with anti-HA-HRP (Roche, 3F10, 1:5000) in 5% milk/TBS ...
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bioRxiv - Microbiology 2022Quote: ... The 10 μL reaction mixture included 5 μL Kapa SYBR Fast Universal 2x qPCR master mix (Kapa Biosystems, Lab Supplies Scientific SA, Hellas), 5 ng of cDNA template ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 800 μg of VLP lysate was then reduced and alkylated as described above and treated with 5 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 12 hours followed by 12 hours with 11 μg of trypsin ...
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bioRxiv - Microbiology 2021Quote: ... and Fwd-P2 (5′-CCATCTCATCCCTGCGTGTCTCCGACTCAGBBBBBBAGARTTTGATCYTGGTTCAG and the reverse primer was Rev1B (5′-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG. The PCR products were sequenced on the 454 GS FLX Titanium sequencer (Hoffmann-La Roche, LTD; Basel, Switzerland). Through the Research Alliance for Microbiome Science Registry (IRB no ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: GST-tagged CBX8 was purified by re-suspending thawed cell pellets in 30 ml of lysis buffer (1× PBS, 5 mM DTT, 1× EDTA free protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN)) per liter of culture ...
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bioRxiv - Cell Biology 2020Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 5 mM EDTA, 50 mM NaF, 1 mM PMSF supplemented with Roche 1X Halt Protease inhibitor cocktail), incubated for 1 hour at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and lysed using immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Approximately 1.5 mg of lysate from each sample was incubated with brachyury antibody at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pooled together and resuspended in 1.5 mL immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Cells were then incubated for 30 minutes on ice prior to centrifugation at high speed (15,000 rpm ...
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bioRxiv - Immunology 2020Quote: ... at 37° C 5% CO2 in 96 well plates in the presence of IL2 (GIBCO 100 IU/mL or Roche 1 ng/mL). Additional conditions included 1 ng/mL TGFβ (GIBCO) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT, 150 mM NaCl, 5 mM MgCl2, 0.001% Triton X-100, 1mM PMSF, 0.2 mM GDP, Roche cOmplete EDTA-free protease inhibitors) and lysed with a microfluidizer ...
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bioRxiv - Cell Biology 2022Quote: ... 1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM Na3VO4×2H2O, 5 µM pepstatin A, 10 µM leupeptin, 2X Roche protease inhibitor cocktail), 200µl glass beads were added ...
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bioRxiv - Plant Biology 2022Quote: ... and then mixed with 4ml protein extraction buffer (50mM Tris/HCl pH 7.5, 5% glycerol, 150mM NaCl, 0.2% triton X-100, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) as soon as the ground tissue had reached room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... protein extraction buffer (50mM Tris/HCl pH 7.5 or HEPES pH 7.5, 150mM NaCl, 5% glycerol, 0.5% NP-40, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) was added to the tissue and grinding was continued until a homogenous suspension was formed ...
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bioRxiv - Biochemistry 2023Quote: ... then lysed in ice-cold NP-40 lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% NP-40, 5% glycerol, Roche cOmplete protease inhibitor cocktail) by trituration followed by incubation on ice for 10 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 7,500 RCF for 15 minutes and then suspended in Lysis Buffer D (20 mM HEPES pH 8, 200 mM NaCl, 5 mM imidazole, 0.5 mM Imidazole, 1x Roche EDTA free protease inhibitor). Cells were lysed using an emulsifier (AvestinEmulsiflexC3 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were suspended in Resuspension Buffer A (20 mM HEPES pH 8, 150 mM NaCl, 5% glycerol, 0.5 mM TCEP, Roche EDTA free protease inhibitor) and lysed using an emulsifier (AvestinEmulsiflexC3) ...
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bioRxiv - Genetics 2023Quote: ... Washed cells were carefully resuspended in 570 μL Buffer A with additives (0.2 mM spermidine, 0.5 mM spermine, 1 mM PMSF, ½ cOmplete ULTRA protease inhibitors tablet, Roche, per 5 mL Buffer A) and permeabilized with 0.1% digitonin in 30 ° C water bath for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-Elav (1:200) (DSHB), mouse anti-β-galactosidase (1:200) (DSHB), Rabbit anti-Wallenda (1:100, (5)) mouse anti-GFP (1:200, Roche, Basel, Switzerland), Rabbit anti-Ref(2)P (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 by quantification of LDH released into cell supernatants by apoptotic/necrotic cells (LDH detection kit, Roche Applied Science, #11 644 793 001). Maximal lysis of the target cells (= 100% ...
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bioRxiv - Neuroscience 2020Quote: ... for 30 min at 4°C on a rotator and S1 (1 ml for cerebellum and 1 ml for cerebrum) was incubated with 5 μg of anti-HA mAb (Roche, clone 12CA5, RRID: AB_514505) at 4°C for 60 min on a rotator ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 5% v/v glycerol, 1 mM TCEP-NaOH, Roche protease inhibitor cocktail EDTA-free) at the ratio of 50 ml / L equiv ...
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bioRxiv - Molecular Biology 2021Quote: ... and homogenized in 5 volumes of ice-cold PBS containing 1% NP-40 and Complete® protease inhibitor cocktail tablets (Roche Diagnostics, Basel, Switzerland) using 2×10 clockwise strokes with 5 s rest time ...