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Citations for Roche :
3301 - 3350 of 3510 citations for 4 Chloro 2 iodothieno 2 3 b pyridine 5 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of sample were used in a reaction mix containing 1 μL 10 μM of each primer (0.5 μM final) and 5 μL 2x Light Cycler SYBR Green LightCycler® 480 SYBR Green I Master (Roche). For telomere amplification ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 0.1 % Triton X-100, complete protease inhibitor cocktail [Roche] added fresh) for 10 min on ice ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were resuspended in binding buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 5 mM TCEP) supplemented with protease inhibitors (Roche cOmplete), sonicated ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by sonication and French Press in purification buffer (50mM sodium phosphate buffer pH 7, 300 mM NaCl) supplemented with 5 ug/mL DNaseI (Roche), 5ug/mL RNAse (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 8, 450 mM NaCl, 1% CHAPS, 20 mM MgCl2, 5 mM ATP, 1 mM Dithiothreitol [DTT], 1X Roche Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were serially applied using the U DISCOVERY 5-Plex IF procedure and the following reagents and kits (all from Ventana Medical Systems, Roche): DISCOVERY inhibitor (760-4840) ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Genetics 2024Quote: ... Blots were then exposed to 2.5 pmol of 40-nt HPLC purified DNA oligonucleotides conjugated to digoxigenin (DIG) using the DIG Oligonucleotide Tailing Kit (Roche) hybridized to the nitrocellulose membrane at 60°C overnight (42°C for 2 h for 5S rRNA ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed using the first-strand cDNAs diluted 5-fold in water and KAPA SYBR FAST qPCR Master Mix (2x) kit (Roche) and gene-specific primers in a LightCycler 96 (Roche).
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Microbiology 2024Quote: ... pH 7.5 and various concentrations of ADP-ribose or hit compounds were mixed on ice in 384-well PCR plate (Roche). Fluorescent signals were measured from 25 to 95℃ in 0.2 C/ 30/Sec steps (excitation ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed in duplicate using 1:20 or 1:5 dilutions of the cDNA and SYBR Green I master mix (Roche) with a LightCycler 480 II (Roche ...
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bioRxiv - Microbiology 2024Quote: ... The heat-denatured RNAs were separated on a 1% agarose gel (95% 1× MOPS buffer and 5% formaldehyde) and transferred to a positively-charged nylon membrane (Roche) by capillary flow ...
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bioRxiv - Neuroscience 2024Quote: ... nuclei were re-suspended in nuclei buffer (PBS, 0,1% Tween 20 in PBS, 5% BSA, 1x Complete EDTA free protease inhibitor cocktail (Roche, 11873580001) and 20U/ml of RNase Inhibitor (Applied Biosystems ...
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Ion channel function of polycystin-2/polycystin-1 heteromer revealed by structure-guided mutagenesisbioRxiv - Biophysics 2024Quote: ... Oocytes were subsequently transferred into 1 mL lysis buffer (Tris 25mM, NaCl 150 mM, EDTA 1mM, NP40 1%, Glycerol 5%, supplemented with Roche cOmplete™ protease inhibitor cocktail ...
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bioRxiv - Immunology 2024Quote: The ear skin was minced finely with scissors and digested in RPMI1640 medium containing 333-666 µg/ml Liberase TL (1 vial with 5–10 mg pack size; Roche), 2 kU/ml DNase I (Merk Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... gene expression (5’Gex) and TCR libraries were generated then quantified via Agilent Technologies 4200 Tapestation and KAPA library quantification kit (Roche). Targeted read depth included 25,000 reads per cell for gene expression libraries and 5,000 reads per cell for TCR libraries ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were then washed three times for 5 min with TBST and developed using the Lumi-Light Western Blotting Substrate (Hoffmann-La Roche). The chemiluminescence signal was detected using a Fusion FX6 reader (Vilber ...
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bioRxiv - Biochemistry 2024Quote: Cells were resuspended in cold lysis buffer (50 mM Hepes pH 8, 50 mM NaCl, 5 mM MgCl2 and 1 tablet 50 mL−1 EDTA-free protease inhibitor, Roche) plus 0.5 mg/mL lysozyme and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... The buffer was aspirated and minced tissue was digested in 5 ml cold HBSS adding 100 μl 10mg/ml Collagenase P (Roche) for 15-20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation (16 h at 4 °C) in the presence of 10 pM TBST containing complete TM protease inhibitor cocktail (Roche Pharma, Basel, Switzerland). The membranes were washed three times with TBS (150 mM NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... pellets were resuspended in 100 µL of lysis buffer (50 mM Tris pH 7.5, 1 mM EDTA, 3 mM DTT, 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche], 1.1 mM PMSF, and 1X Pepstatin A) and beaten on a bead-beater for 5 minutes at room temperature with 100 µL of acid-washed glass beads (cat ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were lysed using Lysis buffer (50 mM HEPES, pH 7.5, 500 mM NaCl, 3 mM TCEP and 1 tablet Roche Protease inhibitor per 10 mL of buffer). Lysate was clarified by centrifugation at 24,000 g for 40 mins at 4 °C ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Coralville, Iowa, USA) and 0.25 µl reverse primer (5 µM, IDT) and was analyzed on a LC480 instrument (Roche, Basel, Switzerland).
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bioRxiv - Cancer Biology 2021Quote: C2C12 cell lysate was generated using 5 million cells in RIPA lysis buffer with protease/phosphatase inhibitors (Roche #11836145001 and #04906837001), and 20μg of protein was loaded on a denaturing SDS page gel for detection of the VGLL2-NCOA2 fusion ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR reactions were conducted in a 10-µL final volume with 5 µL of 2X FastStar Universal SyBR green Master (Roche, Switzerland), 1.5 µL of forward/reverse primer mix (Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... After that pelleted beads were gently resuspended in washing buffer containing 4xTBS, 5% NP-40, phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and washed 10 times with gentle agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 by quantification of LDH released into cell supernatants by apoptotic/necrotic cells (LDH detection kit, Roche Applied Science, #11644793001). Maximal lysis of the target cells (= 100% ...
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bioRxiv - Biochemistry 2020Quote: ... before lysis in swelling buffer (5 mM PIPES pH8.0, 85mM KCl) freshly supplemented with 1x protease inhibitor cocktail (Roche, cat. 04693116001) and 0.5% NP-40 ...
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bioRxiv - Cancer Biology 2020Quote: ... washed twice in PBS and resuspended in 100 μL annexin V incubation buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 5 mM CaCl2) containing 1% annexin V FLOUS (Roche Molecular Biochemicals) and 500 μg/μl PI stain ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissue was then washed with PBST extensively (10 times or more for 5 minutes) before development with BM-purple (1ml peer well, Roche Diagnostics). Time of development was approximately 24 hours at 4°C ...