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Citations for Roche :
301 - 350 of 1905 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... coverslips were treated for 15 min at room temperature with DNAse I (Roche – 25 U/coverslip) then washed with 1 ml of PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and then for 15 min with Liberase TL (0.25 U/ml; Roche Diagnostics Corp., Indianapolis, IN) and papain (30 U/ml ...
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bioRxiv - Zoology 2019Quote: ... These slides were washed four times for 15 min with DIG wash buffer (Roche Diagnostics, Germany). Visualization of fluorescent labelled section was carried out using fluorescent microscope at 480 nm wavelength (Leica Microsystems ...
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bioRxiv - Developmental Biology 2021Quote: ... using the KAPA Dual-Indexed Adapter Set (15 mM, KAPA Biosystems/Roche, #08 278 555 702). Probes for the pull-down were designed according to the Roche Nimble Design software ...
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bioRxiv - Developmental Biology 2021Quote: ... using the KAPA Dual-Indexed Adapter Set (15 mM, KAPA Biosystems/Roche, #08 278 555 702). Probes for the pull-down were designed according to the Roche Nimble Design software ...
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bioRxiv - Cell Biology 2023Quote: ... 15 mM NaCl2 and 1 M sucrose) supplemented with 1x protease inhibitor cocktail (PIC, Roche,11697498001)and 1mM PMSF ...
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bioRxiv - Immunology 2023Quote: ... kidneys were then incubated for 15 minutes in DMEM containing 1 mg/ml Collagenase D (Roche), 100U/ml DNAse I (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription of antisense and sense probes was performed with a DIG-RNA Labelling Mix (Roche, Mannheim, Germany) using either T7 or T3 polymerase.
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by in vitro transcription with DIG-RNA labelling Kit or Fluorescein-RNA labelling kit (Roche, Switzerland). The integrity of probes was determined by gel electrophoresis and storage in 50% RNAlater (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled probes where generated using T7/SP6 transcriptions start sites and the digoxigenin RNA Labeling Mix kit (Roche) after manufactures instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled RNA probes for human NORAD were synthesized by in vitro transcription using a DIG-labeling mix (Roche). Primers used for amplification of the DNA template for each probe are provided in Supplementary File 1 ...
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Half of the reverse transcription reaction was used for amplification with a KAP real-time PCR mixture (KAPA Biosystems) using the Illumina Truseq small RNA library amplification kit primers ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... were produced with DIG-UTP by in vitro transcription using the Sp6 and T7 promoters according to the manufacturer’s protocol (RNA-labeling kit; Roche). The sections were treated as follows ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 ng of cDNA was used for quantitative reverse transcription PCR with the FastStart SYBR Green Master (Roche) and the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
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bioRxiv - Neuroscience 2023Quote: ... In vitro transcription was performed with PCR-amplified templates using T7 RNA polymerase (Hoffman La Roche AG, Basel, Switzerland) to synthesise antisense RNA probes (Supplementary Table 29) ...
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bioRxiv - Developmental Biology 2021Quote: ... Resultant templates were gel extracted then 500 ng transcribed into RNA using T7 RNA polymerase (ThermoScientific, EP0111) and either dig- or FITC-labelling mix (Roche, 3935420 and 32874620 respectively). RNA probes were purified using Microspin G25 (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2019) via 15 rounds of high-fidelity PCR amplification (KAPA HotStart PCR Kit, KAPA Biosystems, Wilmington, Massachusetts). Each pooled ...
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bioRxiv - Genomics 2019Quote: ... We then added 15 µl PCR master mix containing 12.5 µl KAPA HiFi HotStart ReadyMix (KAPA Biosystems)) and 0.5 µl 10 µM PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT) ...
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bioRxiv - Genetics 2020Quote: ... cDNA amplification was done for 15 cycles from pools of 2,000 beads using HotStart ReadyMix (Kapa Biosystems) and the SMART PCR primer ...
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bioRxiv - Cell Biology 2021Quote: ... and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml) (Roche). Damaged livers (48h-72h after APAP injection ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 15 minutes with 4 μL of X-treme Gene HP DNA transfection reagent (Roche). Transfected cells were selected for by hygromycin resistance ...
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bioRxiv - Biophysics 2022Quote: ... then diluted to 0.5 μM in KMEH + 15 KH2PO4 supplemented with 0.01% Nonidet P40 (NP40) substitute (Roche), an additive which we have found improves our ability to achieve thin vitreous ice films ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 mM EGTA; 1.5 mM MgCl2; 150 mM KCl; 15% glycerol; 0.075% NP-40 to which Roche cOmplete™ Mini tablets containing a protease inhibitor cocktail were added just prior to use) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... washed in KPBS and incubated for 15 minutes at 37 °C in presence of proteinase K (Roche) at 0.01 mg⁄mL in an appropriate buffer (0.1M Tris–HCl pH 8.0 ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% SDS) at 50°C for 15 min and finally washed with 20 ml washing buffer (Roche) at room temperature for 5 min ...
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bioRxiv - Genetics 2023Quote: ... We PCR-amplified bisulfite-treated DNA with 15-16 cycles with KAPA HiFi Uracil polymerase (Kapa Biosystems). We used Illumina TruSeq unique index adapters for PCR amplification and multiplexing of the libraries ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl of target RNA were incubated for 15 min with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2 % SDS and 15 mM EDTA) in the presence of protease inhibitors (Roche, Complete Mini, EDTA-free). Blotting was conducted using the Trans-Blot Turbo Transfer System (Biorad) ...
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... thermophila intron were obtained by in vitro transcription of the corresponding linearized plasmids with 20 U of T3 bacteriophage RNA polymerase (Roche) in 20-μl reactions containing 40 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 50 ng DNase-treated RNA was used as template for reverse transcription using either RT Transcriptor from Roche and random hexamers or Moloney Murine Leukemia Virus-RT (MMULV-RT ...
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bioRxiv - Microbiology 2019Quote: ... RNA transcripts of the cloned 5’UTRB7 fragments were produced in vitro from the resulting purified PCR products by using the T7 Transcription kit (Roche) and labeled by using the RNA 3’ end biotinylation kit (Pierce) ...
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bioRxiv - Developmental Biology 2020Quote: ... The RNA concentration was measured by a spectrophotometer (NanDrop1000; Thermo) followed by a reverse transcription process using PrimeScript RT reagent kit (Roche). Real time PCR analysis was performed using SYBR green mix (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were synthesized from cDNA templates via T3 RNA polymerase mediated strand-specific RNA transcription using DIG/FL RNA labelling mix (Roche). Hybridised ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were hybridized with antisense RNA probe for IR84a (nucleotides 82-780) or IR8a (nucleotides 4-529) sequence labelled using in vitro transcription with digoxigenin-11-UTP (Roche). The signals were visualized with a nonradioactive detection system using anti-digoxigenin Fab fragment conjugated to alkaline phosphatase (Roche).
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bioRxiv - Physiology 2021Quote: ... mRNA expression levels were measured after reverse transcription by quantitative real-time PCR (qRT-PCR) with FastStart SYBR Green master mix (Roche) using a LC480 instrument (Roche) ...
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bioRxiv - Plant Biology 2021Quote: ... Gene transcription was quantified by qPCR using a LightCycler 480 SYBR Green I Master kit and LightCycler 480 (Roche, Switzerland). The PCR conditions were 95 °C for 10 min followed by 45 cycles of 95 °C for 10 s ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 200-500 ng of total RNA in the Transcriptor First Strand cDNA synthesis kit (Roche) with 60 μM random hexamer and 6.25 μM oligo(dT)18 primer ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative reverse transcription PCR (RT-PCR) was performed with 10 ng of cDNA in a Light Cycler® Instrument (Roche) using LightCycler® 480 probes master mix (4887301001 ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes (sense and antisense) were generated by transcription in vitro using SP6 or T7 RNA polymerases (Roche) as previously described (Cruz et al. ...
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bioRxiv - Developmental Biology 2022Quote: Antisense RNA probes for in situ hybridization were prepared by in vitro transcription using Digoxigenin- or Dinitrophenyl-conjugated UTP (DIG, Roche 11277073910 ...
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bioRxiv - Cancer Biology 2019Quote: Reverse transcription (RT) reactions were carried out using 1 μg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Roche Diagnostics ...