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Citations for Roche :
201 - 250 of 1905 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The plasmid was linearized and used for in vitro transcription (Roche Dignostics, Indianapolis, IN, USA) to label RNA probes with digoxigenin-UTP ...
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bioRxiv - Neuroscience 2020Quote: All probes were prepared by in vitro transcription using a kit from Roche (Mannheim, Germany) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse primers included a promoter for in vitro transcription using SP6 RNA polymerase (Roche RPOLSP6RO) with incorporation of UTP-digoxigenin (Roche 11093274910) ...
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bioRxiv - Molecular Biology 2022Quote: To determine the repM transcription start site a 2nd Generation 5’/3’ RACE Kit (Roche) was used according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Cat: 04897030001).
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription (1 μg RNA per sample) was performed using M-MuLV reverse transcriptase (Roche) and random 9-mer primers (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Riboprobes were generated by in vitro transcription in the presence of Digoxigenin-UTP (Roche Diagnostics). Antisense and sense Charme probes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription into cDNA was performed using the Transcriptor Reverse Transcriptase kit (Roche, Indianapolis, IN) and random hexamer primers (Roche ...
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bioRxiv - Genetics 2021Quote: ... Two corneas (per mouse) were incubated in 15 mg/ml Dispase (4942078001, Roche) in DMEM (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... 10 kb and 15 kb were constructed using KAPA library prep kit (Roche) and Illumina Mate Pair Library kit (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... sections were blocked for 15 minutes in Blocking Reagent (Roche Diagnostics, room-temperature) and subsequently incubated with primary antibodies anti-Nur77 (NR4A1 ...
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bioRxiv - Neuroscience 2023Quote: ... mechanically dissociated and digested for 15 minutes with liberase (0.4 U/mL; Roche) and DNAse I (120 U/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... 15 mM MgCl2) in the presence of Protease Inhibitor Cocktail (PIC; 5056489001, Roche). Nuclei were pelleted and lysed for 1 h at 4 °C (300 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... The whole transcriptome was amplified (15 cycles) by KAPA HiFi DNA polymerase (Roche), and then size-selected using 0.6X AmpPure XP beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... then 15 μl of 20 mg/ml Proteinase K (Roche #46950800/Sigma #3115887001) per sample was added and the tissue was ground again ...
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bioRxiv - Developmental Biology 2020Quote: ... gbx1 and pax6a digoxigenin and FITC antisense probes were generated from linearized plasmids using an RNA labelling and detection kit (Roche)87 ...
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bioRxiv - Neuroscience 2019Quote: ... serine/threonine kinase A-Raf (Araf) were in vitro transcribed from template cDNA using T7/T3/SP6 RNA polymerase (DIG/FITC RNA labeling kit, Roche) and purified with RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA probes were generated using PCR with T7 promoter sequence linkers and subsequently transcribed [DIG or FITC labeled] using T7 polymerase (Roche). Primer sequences are all found in Table 3 ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was converted into cDNA using the High Capacity cDNA Reverse Transcription Kit (Roche #4368814) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and were immediately subjected to reverse transcription with the Transcriptor First Strand cDNA Synthesis Kit (Roche). Each representative cDNAs of il-6 ...
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bioRxiv - Genetics 2019Quote: ... Probes were synthesized by in-vitro transcription using DIG labeling mix and T7 polymerase (Roche, USA). Hybridization was conducted as previously described (72) ...
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bioRxiv - Neuroscience 2022Quote: ... 1X Transcriptor Reverse Transcription reaction buffer and 10U Transcriptor Reverse Transcriptase (Roche Applied Science, Indianapolis, IN) in 20μL total volume ...
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bioRxiv - Cell Biology 2022Quote: ... Single-stranded Xist riboprobe was generated by in vitro transcription using biotin-16-UTP (Roche, 11388908910) and MAXIscript SP6 kit (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using SYBR green (Roche; #04913914001). Quantification of messenger RNA (mRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... and riboprobes were prepared using the DIG RNA labeling mix and SP6/T7 transcription kit (Roche). Sections were fixed in 4% PFA for 10 minutes ...
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bioRxiv - Developmental Biology 2019Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2021Quote: ... and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
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bioRxiv - Cell Biology 2021Quote: ... and reverse transcribed to cDNA using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). DYNLL1 was tested for splicing defects ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the gene-coding sequences via in vitro transcription using DIG RNA Labeling Kit (Roche, 11175025910). A short bleaching was used to remove the pigments from the fixed zebrafish embryos.
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bioRxiv - Microbiology 2022Quote: ... Digoxigenin (DIG) RNA probes were obtained by in vitro transcription with T7 RNA polymerase (Roche Diagnostics) using PCR-generated DNA fragments as templates ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA synthesis was performed by reverse transcription using the Transcriptor High Fidelity cDNA-Synthesis Kit (Roche) according to the manufacture’s protocol ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... In vitro transcription was carried out at 25 μL scale according to the manufacturer’s instructions (Roche). Reaction products were precipitated by adding 80 μL ice cold ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained after reverse transcription using a First strand cDNA synthesis RT-PCR kit(Roche) and quantitative PCR was performed using SYBR Green (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The probes were generated by in vitro transcription in the presence of Digoxigenin-UTP (Roche Diagnostics). Following hybridization ...
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bioRxiv - Developmental Biology 2022Quote: ... Probes were synthesized by in vitro transcription using the DIG and Fluorescein RNA Labeling Mix (Roche). DIG and fluorescein-labeled probes were detected with anti-DIG or anti-Fluorescein POD-conjugated Fab fragments (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was processed for reverse transcription (RT) using Transcriptor First Strand cDNA Synthesis Kit (Roche). Real-time PCR reactions were carried out using SYBRGreen Master Mix (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... made using 15 μL of the FastStart Universal SYBR-Green Master Mix (Rox, Roche) and 2.5 pmol of each primer ...
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bioRxiv - Plant Biology 2019Quote: ... Seeds were selected on germination medium containing 15 µg ml-1 Hygromycin B (Roche) or 50 µg ml-1 Kanamycin (ProGPT1-GPT1_N-long mat ...
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bioRxiv - Cancer Biology 2021Quote: ... Esophageal tissues were incubated for 15 minutes in Dispase I (Roche catalog no. 04942086001) diluted at 1mg/ml in PBS before separating the epithelium with fine forceps ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue was incubated for 15 minutes in Dispase I (Roche catalog no. 04942086001) diluted at 1mg/ml in PBS before separating the epithelium with fine forceps ...
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bioRxiv - Biochemistry 2019Quote: ... 15 mM imidazole and one tablet of Complete EDTA free protease inhibitor cocktail (Roche). Cell lysate was pelleted for 60 min at 30,600 × g at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... and supplemented with 5 mM CaCl2 and 15 units of S7 micrococcal nuclease (Roche). Lysates were sonicated for 10 seconds at low power followed by incubation on ice for 30 minutes and clarification by centrifugation at 13,000 x g for 15 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 15 mM imidazole) and one tablet of Complete EDTA free protease inhibitor cocktail (Roche). Cell lysate was pelleted for 60 min at 30,600 × g at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... iBAT digestion buffer was prepared as follows: 15 mg collagenase B (Roche, Cat#11088831001) was dissolved in 5 mL of 2X master mix consisting of 125 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Reactions were 30 μL and contained 15 μL KAPA HiFi HotStart ReadyMix (Roche 7958935001), 3 μL of each primer (10uM concentration) ...
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bioRxiv - Immunology 2022Quote: ... Following endogenous peroxidase blocking (15 minutes, ambient temperature, Discovery Inhibitor, Roche, Ventana Medical Systems), tissue slides were incubated using the primary antibodies FPR1 (polyclonal ...
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bioRxiv - Cell Biology 2024Quote: ... 15% glycerol) supplemented with protease inhibitors (1x Complete® EDTA-free (Roche, Cat. # C7698), 1 mM PMSF ...
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bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...