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Citations for Roche :
451 - 500 of 1905 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... high affinity antibody (Roche) and chemiluminescence was visualized using a ChemiDoc gel system (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... Myc (11814150001) antibodies (Roche); phosphorylated IκBα (AF5851) ...
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bioRxiv - Plant Biology 2023Quote: ... All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche; anti-D2 antibody from Agrisera). Coomassie brilliant blue (CBB ...
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bioRxiv - Neuroscience 2022Quote: Primary rat monoclonal antibodies included anti-HA antibody (000000011867431001, 3F10, Roche, Rotkreuz, Switzerland). Primary mouse monoclonal antibodies anti-c-myc antibody (9E10 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody (Ki67) was diluted with Antibody Diluent (Reference: 760-108, Roche) and the samples were incubated 60 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used in this study were mouse anti-BrdU antibody (11170376001, Roche), goat anti-GFP (ab6658 ...
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bioRxiv - Cancer Biology 2021Quote: ... at 95°C for 5 min (90°C for 15 sec, 60°C for 60 sec) × 50 cycles (Light/Cycler Nano, Roche). The primer sets used in this study were described in the supplemental Table 1.
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bioRxiv - Microbiology 2020Quote: ... Any contaminating DNA in the samples was removed by treating 15 µg of RNA with 10 U of DNase I (Roche) for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio) ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and resuspended in 0.5 mL lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg·mL-1 lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany) and incubated at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cell lysate was separated on 15%–45% linear sucrose gradient in presence of 0.1mg/ml Cycloheximide (CHX) and Phosphatase inhibitor (Roche# 4906837001) by centrifugation at 39,000 rpm in SW41 rotor for 90 min ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected DRG neurons were fixed 15 min in 4 % paraformaldehyde (PFA) and hybridized with DIG-labeled GFP cRNA probes (Roche) as described previously (Merianda et al. ...
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bioRxiv - Immunology 2021Quote: ... The reverse transcriptase was inactivated at 70 °C for 15 min and single stranded cDNA was subsequently amplified using Kapa HiFi HotStart Readymix (Roche) at a 1x concentration together with 250 nM UP-primer* (Metabion ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... SE was stopped after 1H by two injections of Diazepam (Valium) with a 15 min interval (10 mg/kg, i.p. Roche, France). Pilocarpine-treated animals were then observed periodically for general behavior and occurrence of spontaneous seizures ...
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bioRxiv - Cell Biology 2021Quote: ... The gel piece was vacuum dried and treated with sequencing grade trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche) at 37°C for 10-16 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... was run on a 15% polyacrylamide gel containing 7M urea and 10 µg/ml APM and then transferred to positively charged nylon membranes (Roche). Pre-hybridization and hybridization steps were performed with Dig Easy Hyb (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... was added to a final concentration of 5X and mixtures were aliquoted to a final volume of 15 µL into a LightCycle 96-well white qPCR plate (Roche). Thermal denaturation was conducted in a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Immunology 2022Quote: ... pursued by 95°C for 3 min and then 45 cycles at 95°C for 15 sec and 58°C for 30 seconds using a LightCycler-480 Real-Time PCR system (Roche). The primers and the probes were designed against the E gene17.
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bioRxiv - Immunology 2019Quote: ... LN were pierced and torn with sharp forceps in 24-well plates and incubated for 15 min at 37° C in 1 ml digestion buffer (100 U/ml collagenase A (Roche), 500 U/ml collagenase D (Roche) ...
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bioRxiv - Immunology 2021Quote: ... the dura mater was carefully removed from the brain and then chopped for enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 15 mg/mL collagenase D (Roche) and 1 mg/mL DNase (Roche ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10s intervals with 1-minute cooling in between) in 15 ml lysis buffer (50 mM Tris, 50 mM NaCl and 1X protease inhibitor cocktail from Roche) at 4° C ...
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bioRxiv - Microbiology 2020Quote: ... followed by 45 cycles of amplification under the following conditions: 94°C for 15 s and 60°C for 60 s in a LightCycler 96 (Roche).
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bioRxiv - Immunology 2020Quote: Age- and sex-matched mice were challenged by intravenous injection of CpG (2 μg premixed with 15 μl DOTAP (Roche) in DPBS ...
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bioRxiv - Microbiology 2021Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 15 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... The solution was incubated for 20 min at 37°C and 15 μl of proteinase K (20 g L-1l; Roche) was added ...
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bioRxiv - Microbiology 2023Quote: ... Any contaminating DNA in the samples was removed by treating 15 µg of RNA with 10 U of DNase I (Roche) for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio) ...
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bioRxiv - Microbiology 2023Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
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bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
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bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
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bioRxiv - Evolutionary Biology 2023Quote: ... lower and upper mouse and hamster first molars were dissected as above and treated for 15 minutes at 37°C with Dispase (Roche) 10mg/ml in Hepes/KOH 50mM ph7.7 ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected in ice-cold PBS and then digested with 4mg/ml Collagenase Type IV/ 0.2mg/ml DNase I /10% FCS/PBS under constant shaking with 700 rpm at 37°C for 12-15 min (Collagenase Type IV, Cat#: 17104-019, Gibco; DNase I, Cat#: 10104159001, Roche). Samples were filtered using a 70µm nylon filter and then washed twice with FACS buffer (0.5% FCS/2mM EDTA in PBS ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 7000 xg for 15 minutes and were lysed in 10 ml PBS supplemented with protease inhibitor (cOmplete, Mini, EDTA-free, Roche). NatT was purified by immunoprecipitation using Anti-FLAG magnetic beads (Merk ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Cell Biology 2024Quote: ... Protein purification started by quickly thawing the cells in ∼15 mL room-temperature 10mM imidazole with protease inhibitor cocktail (Roche) and freshly added 200µM PMSF ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription of 1 µg RNA into cDNA was done using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin-containing RNA probes were generated by in vitro transcription with T7 polymerase using the DIG RNA Labelling kit (SP6/T7) from Roche (cat. No 11175025910). The oligonucleotide AJ529 (T7 promoter ...
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bioRxiv - Neuroscience 2023Quote: ... of the medaka vt cDNA followed by in vitro transcription using T7 RNA polymerase and DIG RNA Labeling Mix (Roche Diagnostics, Basel, Switzerland). The in situ hybridization procedure has been outlined in detail elsewhere (Hiraki-Kajiyama et al. ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from Lipo246 and Lipo815 cell lines and Reverse transcription (RT) reactions were carried out using Transcriptor RT enzyme (Roche Diagnostics, Basel, Switzerland). Nested PCR was performed using a 2-step PCR reaction as previously described(31).
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bioRxiv - Microbiology 2021Quote: ... Bound primary antibodies were detected using biotin-conjugated anti-rat antibody (Roche, 1:1,000) and AlexaFluor594-conjugated streptavidin (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibodies: mouse α-GFP (Roche), rabbit α-RFP (Rockland) ...
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bioRxiv - Developmental Biology 2020Quote: ... alkaline phosphatase-conjugated antibody (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2020Quote: ... rat anti-HA antibodies (Roche) were coupled covalently to protein G-coated magnetic beads (Dynal ...
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bioRxiv - Molecular Biology 2021Quote: ... after which DIG antibody (Roche) was added to the blocking buffer at a ratio of 1:10,000 and incubated for an additional 1/2 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... antibodies and NBT/BCIP (Roche) or INT/BCIP (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-HA antibody (11867423001, Roche) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... mouse anti-GFP antibody (Roche) and the HRP conjugated goat anti-mouse secondary antibody (Bangalore Genei) ...
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bioRxiv - Cell Biology 2021Quote: ... Commercial primary antibodies: EGFP (Roche), Myc (9E10 ...