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Citations for Roche :
3251 - 3300 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... cells were resuspended in 200 μl lysis buffer (10 mM Tris/Cl pH 7.5; 150 mM NaCl; 500 µM Ca2+, protease inhibitors (Roche), 2% NP-40) ...
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bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
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bioRxiv - Plant Biology 2019Quote: ... Seedlings were ground in liquid nitrogen and homogenized in ice-cold extraction buffer (25 mM HEPES-KOH pH 7.5, 10 mM MgCl2, protease inhibitor cocktail [complete Mini, Roche] ...
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bioRxiv - Plant Biology 2019Quote: ... Seedlings were ground in liquid nitrogen and homogenized in ice-cold extraction buffer (25 mM HEPES-KOH pH 7.5, 10 mM MgCl2, protease inhibitor cocktail [complete Mini, Roche], phosphatase inhibitor cocktail [phosSTOP, Roche]). Samples were incubated on ice for 10 min and then centrifuged for 15 min at 16,000 g ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in Triton X-100 lysis buffer (1% Triton X-100 in TBS, pH 7.5) containing 10 μM Leupeptin (Roche) and 1 mM Pefabloc SC (Roche) ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Microbiology 2020Quote: ... 300 mM NaCl and 10 mM imidazole pH 8.0) supplemented with a cOmplete™ protease inhibitor cocktail tablet (Roche). Cell lysis was performed by sonication and the lysate was centrifuged at 20,000 rpm for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell extracts were prepared by rotating the cell pellets for 2 hrs at + 4°C in five volumes of the high salt lysis buffer (50 mM Tris, 300 mM NaCl, 10% glycerol, 0.5% NP-40, 1x cOmplete ULTRA protease inhibitors (Roche)) ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... pH 7.5, 150mM NaCl, 10% glycerol, 5mM MgCl2, 0.1% IGEPALl, 5mM DTT, and 1x cOmplete™ Protease Inhibitor Cocktail [Roche]), and incubated for 20 minutes at 8 rpm in the cold room ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... 150mM NaCl, 10% glycerol, 5mM MgCl2, 0.1% Tween20, 15mM EGTA, 10μM MG132, and 1× cOmplete™ Protease Inhibitor Cocktail [Roche]) and incubated for 30 min at 8 rpm in the cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Triton X-100) supplemented with Complete® protease inhibitor cocktail (Roche, 1 pastel per 10 mL lysis buffer) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR reaction mixture (25 μl total volume) comprised of 10 μl of 2X KAPAHiFi HotStart Mix (Roche, Switzerland), 5 μM each of 341f and 805r primers and 5 μl of DNA template which was ≥20Lng/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets resuspended in LB1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2 supplemented with protease inhibitors [Roche]) for 5 minutes at 4 °C followed by centrifugation (1,000g ...
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bioRxiv - Pathology 2021Quote: ... 10 mM Na4P2O7) in the presence of protease inhibitors (completeTM, Mini, EDTA-free Protein inhibitor cocktail tablets, Roche, Sigma). Gut tissue and GDE were homogenized in the lysis buffer and sonicated three time for 30 seconds each at 10 μm amplitude ...
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bioRxiv - Neuroscience 2020Quote: ... CAD5 cells were incubated with (0.01%) non-infectious brain homogenate (10% w/v in 0.32M sucrose) to control for efficient proteinase K (PK) (Roche) digestion and to compute the background of the assay ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR reactions were performed in a total volume of 10 μL of SYBR Green master mix I (Roche) in 384-wells plates on a Lightcycler LC480 apparatus (Roche ...
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bioRxiv - Biophysics 2019Quote: ... 4 million cells were seeded in a 10 cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM Na3VO4 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 0,25 % NP-40 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated on a rocker for 10 minutes with 0.4 mL RIPA lysis buffer containing protease inhibitors (cOmpleteTM tablets, Roche) and phosphatase inhibitors (PhosSTOPTM tablets ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris/HCl pH 7.4/ 50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Immunology 2021Quote: ... Livers and liver tumours were excised and then minced with scissors before incubation in RPMI/10% (v/v) FBS/2mM EDTA/1.2mg/mL Collagenase D (Roche)/1mg/mL DNase I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The cell pellets were mixed with 10-fold volume of sucrose buffer (0.25 M sucrose; protease and phosphatase inhibitor cocktails (Roche)) and homogenized until a completely homogenous solution was obtained ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM NaCl; 20 mM Tris-HCl [pH 7.5]; 1 mM EDTA, 10% glycerol; complete protease inhibitor cocktail [Roche]). Samples were mixed (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting pellets were re-suspended in Lysis buffer (300mM NaCl, 50mM Tris pH 7.5, 10% Glycerol) containing the protease inhibitor cocktail (Roche), and lysed using sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... Dechorionated embryos were then washed once with cold DEI buffer (10 mM HEPES pH 7.4, 5 mM DTT and 1x complete protease inhibitor from Roche) followed by homogenization in 1-2 embryo volumes of DEI buffer on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... and resuspended in SDS lysis buffer (50 mM Tris-HCl [pH 8.1], 1% SDS, 10 mM EDTA) with complete protease inhibitor cocktail (Roche). Cell extracts were sonicated with a Bioruptor TOS-UCW-310-EX (output ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Genomics 2021Quote: ... These cells were re-suspended in lysis buffer (4% SDS, 50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 100 mM NaCl) with Protease inhibitor (Roche) and RNase inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were pooled by stage and underwent three rounds of centrifugation at 1500rcf at 4°C for 10 minutes followed by rehydration with DPBS with 0.5% non-acetylated BSA and 0.5U/μl RNAse inhibitor (Roche 3335399001).
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bioRxiv - Immunology 2021Quote: ... then incubated for 45 min with constant shaking with “digest buffer” (RPMI; 10% FBS; 1mg/ml collagenase/dispase (Roche); 20μg/ml DNAse 1 (Sigma)) ...
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bioRxiv - Molecular Biology 2021Quote: (Thawed) Cell pellets were lysed on ice for at least 10 min with 1x protease inhibitors cocktail (PIC; Roche) and 1x quenchers (10 mM NaN3 ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl; 10 mM EDTA; 0.1% SDS; 1% Triton X-100; 1% deoxycholate; 1x complete protease inhibitor cocktail [Roche]), clarified and 20 μg total protein were used for SDS-PAGE followed by electroblotting ...
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bioRxiv - Immunology 2021Quote: B16F10 cells expressing ovalbumin (B16-ova, (Dieterich et al., 2017)) were cultured in DMEM supplemented with 10% FBS and 1.5 mg/ml G418 (Roche).
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bioRxiv - Molecular Biology 2021Quote: ... washed twice in cold PBS and resuspended in lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.1, supplemented with protease inhibitor cocktail, #11873580 001, Roche). Chromatin fragmentation was performed using Soniprep 150 to produce a distribution of fragments enriched between 300 and 400 bp ...
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bioRxiv - Immunology 2020Quote: ... The first PCR was performed in 10 μl and consisted of 1x KAPA HiFi Hot Start Ready Mix (Roche), each 16S rRNA primer at 0.2 μM and 3-μl DNA ...
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bioRxiv - Immunology 2021Quote: ... Add 120 ul of fresh ChIP SDS Lysis Buffer (0.5% SDS, 50 mM Tris, pH 8, 10 mM EDTA, 1 × protease inhibitor cocktail (Roche)) per 5 million cells ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... a 1% agarose gel block embedded with enterococci was treated with lysozyme solution (10 mg/mL; Roche, Basel, Switzerland) at 37 °C for 6 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP and 10 % glycerol) supplemented with 2x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed with SLB (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001 ...
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bioRxiv - Genomics 2022Quote: ... 25 mM potassium chloride, 5 mM magnesium chloride, 10 mM Tris-HCl pH 8.0, 1 μM DTT, 1x Roche Protease Inhibitor Cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested and re-suspended in buffer A supplemented with 10 μM ZnCl2 and protease inhibitor (Complete, Roche). Cells were then homogenized by sonication and the crude extract was centrifuged for 30 min at 18,000□×□g ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
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bioRxiv - Biochemistry 2022Quote: ... 10 min), resuspended in lysis buffer (10% glycerol, 20 mM HEPES pH 7.4, 200 NaCl, cOmplete protease inhibitor mixture (Roche), 1 mg/mL DNAse I (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer2 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche), 0.5 % IGEPAL CA-630 ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...