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Citations for Roche :
3201 - 3250 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were isolated by adding SDS lysis buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol) supplemented with protease inhibitor cocktail (PIC; Roche). DNA was sheared by sonication for 10 min at high settings (30 s on ...
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bioRxiv - Cell Biology 2020Quote: ... were resuspended in 1x sample buffer (0.5 x TBE, 10% glycerol, 2% SDS) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (5 mM sodium fluoride ...
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bioRxiv - Plant Biology 2021Quote: ... Each 10 μl reaction was comprised of 5 μl LightCycler 480 SYBR Green I Master mix (Roche Life Science), 2 μl cDNA template ...
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bioRxiv - Plant Biology 2020Quote: ... Cell pellets from 200 mL of Chlamydomonas culture were resuspended in an extraction buffer (20 mM HEPES pH= 7.5, 20 mM KCl, 10% glycerol, 2× EDTA-free protease inhibitor mix (ROCHE)) ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondria were resuspended in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2, containing 1X protease inhibitor cocktail from Roche).
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 10 ml buffer A (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM MgCl2, 5 mM ATP, Roche cOmplete EDTA-free protease inhibitor catalog # 11872580001) ...
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Plant Biology 2020Quote: ... and homogenized in 100 μl ice-cold homogenization buffer (1% [v/v] SDS, 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 10 mM DTT and a Roche Complete protease inhibitor 1 tablet/10 ml) ...
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bioRxiv - Microbiology 2021Quote: ... resuspended with 1 mL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl PH7.0, freshly added 1 mM PMSF, complete protease inhibitor [Roche]) for 10 min at 4°C and subjected to sonication to fragment the genomic DNA in size range of 300-700 base pair ...
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bioRxiv - Microbiology 2021Quote: ... pH 8 containing 150 mM NaCl, 1% Nonidet-P40, 0.1% SDS, 10 mM NaF, and EDTA-free protease inhibitor cocktail [Roche]). Cellular debris and bacteria were removed by centrifugation and collected as the bacterial fraction ...
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bioRxiv - Microbiology 2021Quote: Collected cell pellets were lyzed in a lysis buffer (40 mg/ml SDS, 100 mM Tris-HCl pH 8.6, 10 mM EDTA and Complete protease inhibitors (Roche)) and sonicated 3 times for 30 s at 40% amplitude ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in 10 μl reactions containing 4 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of PCR-grade water (Roche) ...
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bioRxiv - Plant Biology 2021Quote: ... 150 mM NaCl, 10% glycerol, 0.2% NP-40, 1 mM EDTA, 5 mM DTT, and EDTA-free protease inhibitor [Roche]). Anti-GFP agarose resin (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... 109 CFUs of bacteria were resuspended in 150 µL of buffer TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) with 5 mg mL-1 of lysozyme (Roche) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.3 % NP-40 and 1 x protease and phosphatase inhibitors, Roche, USA) by passing through a 23G needle and syringe 30 times and then left to stand on ice for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... 25 mM NAF and 10 μM ZnCl2 which was supplemented with 1 x cOmplete protease inhibitor cocktail (Roche, 11836170001), 1 mM PMSF (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... cells from mouse blood infected with the DEH-GFP-expressing parasite were pelleted and then lysed in hypotonic buffer (10 mM Tris-HCl pH 8.4, 5 mM EDTA) containing protease inhibitors (Roche), freeze/thawed twice ...
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bioRxiv - Cell Biology 2020Quote: ... 90 mM KCl, 2 mM EDTA, 0.5 mM EGTA, 10% Glycerol, 2 mM DTT, and 1x complete protease inhibitors Roche) was added ...
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bioRxiv - Cell Biology 2020Quote: ... 500 mM Tris pH 7.4, 20 mM EDTA, 10 mM NaF, 2 mM benzamidin and protease inhibitor cocktail [Roche]), and centrifuged 3 min at 5000 rpm ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellet samples were collected and re-suspended in lysis buffer (100 mM Tris-HCl, pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set ...
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bioRxiv - Cell Biology 2020Quote: ... cell nuclei were counterstained with 100 ng/ml of DAPI for 10 min at room temperature (Roche Applied Science). Slides were mounted on coverslips using Fluoromount mounting medium (DAKO) ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was retrieved into a new 2 ml Eppendorf tube and diluted 10 times with RIPA buffer 0% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, protease inhibitor cocktail (Roche)) to obtain a concentration of 0.1% sodium dodecyl sulfate ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM MgCl2, 10% glycerol, 300 mM NaCl, 0.2 U/mL DNase 1, 1 mM PMSF, complete proteinase inhibitor mixture; Roche) and homogenized with an EmulsiFlex for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Biochemistry 2022Quote: ... the cells from each dish were then suspended in 300 μL of isotonic sucrose buffer (290 mM sucrose, 10 mM imidazole, pH 7.0, 1 mM DTT, and 1 cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... The crude nuclei pellet was washed in 500 μL of isotonic sucrose buffer (290 mM sucrose, 10 mM imidazole, pH 7.0, 1 mM DTT, and 1 cOmplete protease inhibitor cocktail (Roche)) supplemented with 0.15% NP-40 ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended with precooled 1 mL isotonic sucrose buffer (290 mM sucrose, 10 mM imidazole, pH 7.0, 1 mM DTT, and 1 cOmplete protease inhibitor cocktail (Roche) per 10 mL buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... in lysis buffer (50 mM Tris–HCl pH 7.5, 300 mM NaCl, 10 mM imidazole, 5 mM β-mercaptoethanol, 0.5% Triton X-100, Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich)) ...
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bioRxiv - Biophysics 2022Quote: ... either 10 μl of vehicle or 10 μl of S1P at different concentrations in 0.5 %w/v fatty acid-free BSA (10775835001, Roche) solution in PBS was added ...
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bioRxiv - Genetics 2022Quote: ... 1 mM MgCl2 10 µM ZnSO4) the buffer was also supplemented with both cOmplete EDTA-free protease inhibitor (Roche) and Benzonase (Merck) ...
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bioRxiv - Molecular Biology 2022Quote: ... β-DDM in 60 ml of 20 mM Bis-tris propane pH 8.0 with 10 % glycerol and EDTA-free Complete protease inhibitors (Roche) for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were harvested from one 10cm dish and resuspended in 1.5× pellet volumes of H-100 buffer (containing 50mM Hepes, pH 7.9, 1mM EDTA, 100mM KCl, 10% glycerol, 1mM MgCl2, and a complete protease inhibitor tablet [Roche]). Cells were ground in liquid N2 with a precooled mortar and pestle ...
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bioRxiv - Cell Biology 2022Quote: ... The protein lysate was electrophoresed into 6-10% SDS-PAGE gels and transferred to a PVDF membrane (Roche, 03010040001). After blocking with 5% BSA ...
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bioRxiv - Plant Biology 2021Quote: ... The final products were purified and enriched via 10 cycles of PCR using KAPA HiFi HotStart ReadyMix (Roche, Switzerland) and primer mix from a Swift Library kit to create the final double-stranded cDNA library ...
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bioRxiv - Plant Biology 2021Quote: ... The final products were purified and enriched with 10 PCR cycles using the KAPA HiFi HotStart ReadyMix (Roche, Switzerland) and primers mix from the Swift library kit ...
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bioRxiv - Plant Biology 2021Quote: ... The RT-qPCR was set up as a 10 μL reaction using Lightcycler 480 SYBR Green Master Mix (Roche) in a 384-well plate ...
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bioRxiv - Immunology 2020Quote: Lungs were aseptically isolated from mice and incubated at 37°C in 10% FBS RPMI media containing 1.4 mg/ml collagenase A (Roche) and 30 μg/ml DNase I for 60 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the embryos were washed 10 times with PBST and the signal was developed in NBT/BCIP solution (Roche). For double in situ hybridization ...
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bioRxiv - Molecular Biology 2020Quote: ... before treating with lysis buffer (10 mM Tris pH 7.5, 2 mM EDTA) containing a protease and phosphatase inhibitor cocktail (Roche). Chromatin-enriched protein fractions were prepared essentially as described (Mailand and Diffley ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 1 mM EGTA, 10% sucrose, 0.8 M NaCl containing sarkosyl (final 1%, w/v) and protease inhibitor (Roche). After ultracentrifugation at 135,000 × g for 20 min at 25 °C ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 10 times and 1 μl was used in 5-μl PCR reaction on a LightCycler 480 (Roche Diagnostics) with Luna Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche)) and incubated for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: Crushed liver powders were lysed with lysis buffer (10 mM Tris-HCl pH8.0, 100 mM KCl, 2.5 mM MgCl2, 0.5% TritonX-100, cOmplete (Roche, Basel, Switzerland). The protein concentration was determined using the BCA protein assay kit (TaKaRa ...
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bioRxiv - Neuroscience 2019Quote: Microdissections were triturated in 100 μl of lysis buffer (150 mM NaCl, 1% Triton, 100 mM Tris, pH 7.4, 10 mM EDTA, 1X protease/phosphatase inhibitor (Roche)) per 10 mg of tissue and incubated 10 min on ice ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 additional hours cells were lysed in mild detergent (10% glycerol 25mM NaCl, 20mM HEPES pH 7.0, 1% DDM, + 1x protease inhibitor (Roche)) ...
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bioRxiv - Biochemistry 2019Quote: Cells were lysed in native lysis buffer (10% glycerol, 25mM NaCl, 20mM HEPES pH 7.0) + 1%DDM and protease inhibitor (Roche) and solubilized by rotating 30m at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl [pH 7.5], 10 mM NaCl, 1% IGEPAL CA-630, 1mM DTT, 1.5mM MgCl2, 1X Protease inhibitor [PI, Roche # 11836170001]) and incubated on ice for 30 min ...