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Citations for Roche :
3151 - 3200 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... libraries were quantified with the KAPA library quantification kit (Roche #07960298001). Libraries were sequenced on Illumina NextSeq 500 sequencer (75-bp paired-end reads ...
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bioRxiv - Immunology 2024Quote: ... Stranded mRNA libraries were prepared a KAPA mRNA HyperPrep Kit (Roche) and sequenced on the Illumina HiSeq 4000 platform (75bp single-end sequencing).
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bioRxiv - Immunology 2024Quote: ... The library preparation was performed using the KAPA Hyperplus kit (Roche) according to the manufacturer’s protocol using unique double index adapters (Roche) ...
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bioRxiv - Immunology 2024Quote: ... The libraries were quantified using the KAPA Library Quantification Kit (Roche) and pooled for sequencing in NovaSeq 6000 (one lane ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were quantified using the KAPA Library Quantification Kit (Roche 7960140001) before mixing ...
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bioRxiv - Microbiology 2024Quote: ... along with a LightCycler 480 SYBR I Master kit (Roche, Switzerland) and Roche consumables ...
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bioRxiv - Microbiology 2020Quote: ... whereas for site 15 we used 0.7 g of sediment per sample and diluted the final extracts 10 times in ultrapure PCR water (Roche). DNA templates were used in quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were disrupted by sonication in a lysis buffer containing 10 mM Tris-HCl pH 7.4 (buffer A) and Complete Protease Inhibitor Cocktail (Roche). Cell debris was removed by centrifugation at 16,000 g at 4°C during 10 minutes and supernatants were collected as total extracts ...
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bioRxiv - Molecular Biology 2019Quote: ... mixed-stage transgenic worms expressing CDE-1::GFP were resuspended in equal volumes of 2× lysis buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche®cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Developmental Biology 2021Quote: ... in liquid nitrogen in a 1.5-ml Eppendorf tube and resuspended in ice-cold lysis buffer (50 mM Tris pH 7.6, 10 mM EDTA, 150 mM NaCl, 1% Triton, supplemented with complete protease inhibitor, Roche Ref ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was lysed with 20 ml of Eps8L2 Lysis Buffer (10 mM Tris.HCLpH8, 150 mM NaCl, 1 mMEDTA, lysozyme 0.1 mg/ml, Pefabloc® (Roche) 0.5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma], 1x Complete EDTA-free Protease inhibitor [Roche]) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 50 mM Tris/HCl pH 7.4/50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... then 10-fold diluted in TBS buffer (50mM Tris-HCl pH 7.5; 150mM NaCl) supplemented with cOmpleteTM protease inhibitors (Roche). ANTI-FLAG M2 Magnetic Beads (SIGMA ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were separated on 10%-15% SDS-PAGE gels and transferred to PVDF membranes (Roche, 03010040001). The membranes were probed with the indicated primary antibodies followed by the appropriate HRP-conjugated secondary antibodies ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The lysis was performed in RIPA buffer (1% SDS, 10 mM Tris pH 8.0, containing protease and phosphatase inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton X-100 and 0.1% SDS) with a protease inhibitor cocktail (Roche/1 tablet in 10 mL RIPA). Homogenates were centrifuged for 5 min at 4°C at 13,000 x G ...
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bioRxiv - Molecular Biology 2021Quote: MtDNA copy number was analyzed in duplicate by quantitative real-time PCR using 1 μl of 1/200-diluted DNA in a 20 μl reaction containing 0.2 μM forward and reverse primers and 10 μl of 2x SyGreen Mix (qPCRBIO #PB20.14-05) in a LightCycler 96 instrument (Roche). Primer pairs targeting cytochrome B (nt 14,682–14,771 of mtDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... 1.5 mM, 10% glycerol, pH 7.4, containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of PCR gene specific primer (10X conc) (Table S1) and 10 μl of master mix (Roche 04707516001) in 20 μl reaction ...
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bioRxiv - Molecular Biology 2020Quote: 5 μl of antiserum were diluted with 250 μL of PBS containing 0.05% NP-40 and coupled to 10 μl of Protein A-Agarose beads (Roche) by rotating at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were incubated with 50 μl of 1:10 Enzyme:TUNEL reagent (TdT and fluorescein-dUTP) (Roche, Cat No. 11684795910) at 37°C for 3 h in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... 50-70 pairs of freshly dissected ovaries were lysed with a douncer in 500μl lysis buffer (0.2% NP40, 20 mM Tris pH7.4, 150 mM NaCl, 10% glycerol) supplied with protease inhibitor (Roche, 11836170001) and 20 mM deSUMOylation inhibitor N-Ethylmaleimide (Sigma ...
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bioRxiv - Physiology 2019Quote: ... Tissue was placed in ice-cold hypotonic lysis medium (HLM, 10 mM Tris, pH 7.4, 1 mM EDTA) in the presence of a protease inhibitor cocktail (Roche), minced and homogenized by 20 strokes in a glass homogenizer ...
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bioRxiv - Plant Biology 2019Quote: ... 10 nM target-specific primers (Table S5) and LightCycler 480 SYBR Green I Master (Roche Molecular Systems, Pleasanton, CA) in the LightCycler 480 II detection system (Roche Molecular Systems ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR reaction (20 μL) consisted of 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant was separated from lysates by centrifugation at 20,000×g for 10 minutes and used for immunoprecipitation using 20 µg of anti-GFP antibody (11814460001, Roche) (used for GFP-CENP-ACnp1 and Hap2-GFP IP ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then resuspended in 10 mM Tris-HCl buffer (pH 7.4) containing protease inhibitor cocktail (cOmplete Tablets, EDTA-free, Roche) after which cell lysis was performed with glass beads (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 0.02% NP40 and 1 mM PMSF ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48 h and resuspended in lysis buffer (50 mM HEPES pH 7.2, 200 mM NaCl, 10% glycerol, 2 mM DTT, and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed using a microfluidizer ...
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bioRxiv - Microbiology 2019Quote: ... The parasites were then lysed in 300 µL of cold hypotonic buffer (10 mM HEPES, pH 7.5) supplemented with protease inhibitors (Roche), passed through 25G needle (5x ...
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bioRxiv - Microbiology 2019Quote: ... The parasites were then lysed in 300 µL of cold hypotonic buffer (10 mM HEPES, pH 7.5) supplemented with protease inhibitors (Roche), passed through 25G needle (5x ...
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bioRxiv - Molecular Biology 2019Quote: ... Every 10 million cells were lysed by 300μL 1% SDS include freshly made Protease Inhibitor (PI) cocktail (Roche #4693132001), sonicated with Diagenode Bioruptor Pico and diluted with 2.7mL PBA (1x PBS + 0.5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... and pellets were disrupted by sonication in PBS buffer (pH 8.0) containing 10 mM EDTA as well as protease inhibitors (Roche) and ...
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bioRxiv - Developmental Biology 2020Quote: ... Pooled ventral otocysts were homogenized in lysis buffer (20 mM HEPES KOH, pH 7.4, 150 mM KCl, 10 mM MgCl2, EDTA-free protease inhibitors [Roche, 04693159001] ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 mM KCl, 0.05 mM EDTA, 10% Glycerol, 1 mM DTT, 1 mM PMSF, 1× Complete Mini protease inhibitor, Roche) resuspended in RIPA buffer (150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-CAGACACGCAGACTCTTTC-3’) and 2.5 µl reverse primer (10 µM; 5’-CTAAAGATGTGTGTCTTCCTCA-3’) using the Expand Long Template PCR System (Roche). The PCR conditions were 35 cycles at 95°C for 30 sec ...
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bioRxiv - Neuroscience 2021Quote: Frozen mouse hippocampi were homogenized in 0.2 ml of pre-cooled homogenization buffer (0.3 M sucrose, 10 mM MOPSNaOH and 1mM EDTA) supplemented with complete protease inhibitor cocktail (Roche). 50 µl of lysis buffer (8% SDS and 0.2 M DTT in 0.2 M Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... pellet was resuspended in lysis buffer (10 mM phosphate buffer saline pH 7.3, supplemented with cOmplete protease inihibitor, EDTA free (Roche) and 1 mM Phenylmethylsulfonyl fluoride (Sigma)) ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were resuspended in 20 mM Tris 1M NaCl 10 mM imidazole pH 8.0 with one EDTA-free protease inhibitor tablet (Roche) and lysed using an Emulsiflex C3 (Avestin) ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 4 hr and washed two times with its resuspending buffer and once with E100 buffer (25 mM HEPES-KOH pH7.5, 10 % glycerol, 100 mM KCl, 2 mM MgCl2, 1 mM EDTA with Roche cOmplete™ ...
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bioRxiv - Neuroscience 2021Quote: ... pH = 7.6; 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Nonidet P40 Substitute; protease inhibitor cocktail [Roche]; PhosSTOP [Roche]), using a Dounce homogenizer ...
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bioRxiv - Cell Biology 2021Quote: ... in sonication buffer (10 mM Tris-Hcl pH 8.0, 0.25% SDS, 2mM EDTA, 1x Roche complete Protease Inhibitor (Roche # 11697498001) with setting “high”,15 x (30 sec on / 30 sec off) ...