Labshake search
Citations for Roche :
3251 - 3300 of 3367 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.). After ligation ...
-
bioRxiv - Microbiology 2024Quote: ... The engorged individuals were maintained for two days at 25°C and then stored in tissue lysis buffer (Roche, Prague, Czech Republic) at - 20°C in pools of 5 females for subsequent DNA isolation and nested PCR.
-
bioRxiv - Microbiology 2023Quote: ... LAMP amplification reactions were heated at 65° C for an hour and a half in either a LightCycler 96 (Roche, NC, U.S.A.) or a heat block ...
-
bioRxiv - Genomics 2023Quote: ... 3 million crosslinked cells were resuspended in 1 mL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, and 1× Roche protease inhibitors Cat#11697498001) and rotated at 4 °C for 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: Cell lysates for intracellular chemokine quantification were obtained via repeated freeze-thaw cycles at −80°C of cells suspended in media containing protease inhibitor cocktail (Roche Applied Science) and final centrifugation at 8000g to pellet debris ...
-
bioRxiv - Immunology 2023Quote: Pelleted crypts were kept at -80°C and lysed with RIPA buffer supplemented with fresh cOmplete™ protease inhibitor cocktail (Roche 11836170001) and PhosSTOP™ phosphatase inhibitor cocktail (Roche 4906837001) ...
-
bioRxiv - Developmental Biology 2023Quote: ... under agitation and then digested at 37°C for 30 minutes with Liberase (0.15mg/ml Liberase TL-Roche 246749-in HBSS/PBS). For both skin and intestine ...
-
bioRxiv - Neuroscience 2023Quote: ... and the striatal slices were incubated at 30°C in Krebs-HCO3-buffer (Mg2+ free) with 10 μg/mL adenosine deaminase (Roche, Basal, Switzerland) for 30 min under constant oxygenation with 95% O2/5% CO2 ...
-
bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein pellet was resuspended in 100ul of Lysis Buffer (150mM NaCl, 4%SDS, 50mM TrisHCl pH 8.0, 10mM EDTA, complete protease and phosphatase inhibitor cocktails-Roche diagnostics).
-
bioRxiv - Microbiology 2021Quote: ... and incubated with blocking buffer (4 % skim milk in 1 × PBS) followed by incubation with the primary antibody: (monoclonal anti-GFP mice 1:5000 (Roche), rabbit anti-GFP 1:10000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... and a potential large inversion on chromosome 4 (Supplementary Table 11) were labeled with digoxigenin-11-dUTP (Roche, Indianapolis, IN), biotin-16-dUTP (Roche) ...
-
bioRxiv - Immunology 2019Quote: ... Tissue sections were placed on charged glass slides and fixed with 4% PFA in 2xPBS with 2x Complete protease inhibitor (Roche) for 10 min ...
-
bioRxiv - Biochemistry 2021Quote: ... centrifuged with 306 x g for 4 min and resuspended in PBS supplemented with EDTA-free protease inhibitor (Roche, 11873580001). Cell disruption was performed using a UP50H ultrasonic processor (Hielscher ...
-
bioRxiv - Neuroscience 2020Quote: ... Transfected DRG neurons were fixed 15 min in 4 % paraformaldehyde (PFA) and hybridized with DIG-labeled GFP cRNA probes (Roche) as described previously (Merianda et al. ...
-
bioRxiv - Bioengineering 2021Quote: Cell-seeded scaffolds were collected at weeks 0 and 4 and digested in proteinase K solution (0.5 mg/ml in phosphate buffer, pH 6.5, Roche, Basel, Switzerland) for 48 hours at 56 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were hybridized with antisense RNA probe for IR84a (nucleotides 82-780) or IR8a (nucleotides 4-529) sequence labelled using in vitro transcription with digoxigenin-11-UTP (Roche). The signals were visualized with a nonradioactive detection system using anti-digoxigenin Fab fragment conjugated to alkaline phosphatase (Roche).
-
bioRxiv - Pathology 2021Quote: ... and homogenized to 20% w/v in PMCA I buffer (1x PBS 150 mM NaCl, 4 mM EDTA) with cOmplete protease inhibitor (Roche). Samples were homogenized on a BeadBlaster for 10 rounds ...
-
bioRxiv - Cancer Biology 2020Quote: ... were co-transfected with 2ug of the Cas9/sgRNA vector PX458 and 4 ul of ssODN HDR template (20 uM) using Xtreme gene (Roche). Two days following transfection ...
-
bioRxiv - Biochemistry 2021Quote: ... Then qPCR was performed in triplicate using 4 μl sample in 8-9 μl reaction with FastStart Universal SYBR Green Master Mix (Roche) and primers flanking each MseI cassette (Table S3).
-
bioRxiv - Cell Biology 2021Quote: ... 500 mM NaCl, 4 mM β-mercaptoethanol, 10 mM ATP, 2.5 mM MgCl2, cOmplete EDTA-free protease inhibitor cocktail-Roche). The beads were eluted with NTA elution buffer (1x PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... and RNAseq libraries (2 biological replicates per condition comprised of a pool of 4 PA-gel cultures each) prepared using KAPA mRNA HyperPrep Kit (Roche) and IDT dual indexed sequencing adaptors ...
-
bioRxiv - Cell Biology 2022Quote: All controls contained 4% (v/v) 25x concentrated protease inhibitor cocktail solution (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) in accordance with the PPP samples.
-
bioRxiv - Cell Biology 2022Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). The lysates were then resuspended in 5 x SDS-PAGE loading buffer containing 25mM DTT ...
-
SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: PCR products were adjusted to 4 × 109 molecules/μl before generating DNA pools that were purified using Kapa Pure Beads (Kapabiosystems, Roche), and quantified using Qubit as previously described (38–40) ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.4 mM phenylmethylsulfonyl fluoride (PMSF) and 4 µM pepstatin) and digested with the chosen endoglycosidase (PNGase F, New England Biolabs; or Endoglycosidase H, Roche) in a small volume of the appropriate buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were washed once with room temperature PBS and then lysed in a solution of 1 volume 5x SDS-PAGE sample buffer (containing fresh 5% (v/v) beta-mercaptoethanol) to 4 volumes of Immunoprecipitation buffer (IPB; (Thomas et al. 2012)) containing fresh Protease Inhibitor Cocktail (Roche) and 1 μM microcystin-LR ...
-
bioRxiv - Cancer Biology 2019Quote: ... was designed to tile a 250 bp capture region within the central 4-kb target region from each 10-kb window of hg19 avoiding non-specific sequences by Roche so that the maximum distance between 2 capture regions is 14-kb ...
-
bioRxiv - Pathology 2020Quote: Lungs harvested and cut into small pieces were digested (1h, 37c) with 4 mg/ml collagenase D (Roche, Mannheim, Germany). Tissue was meshed through a 40-µm cell strainer ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cells were treated with 10 μmol/L of MG132 for 4 h before being lysed on ice in the RIPA buffer supplemented with a proteinase inhibitor cocktail (Roche). The cell lysates were precleared with 20 μl of protein A/G Agarose (SantaCruz) ...
-
bioRxiv - Neuroscience 2019Quote: ... After the 4-hr period of BDNF treatment, cells were washed once with ice-cold PBS solution (PBS+EDTA, 1x PhosStop (Roche) and 1x Protease Inhibitor cocktail (Roche cOmplete protease inhibitor) ...
-
bioRxiv - Microbiology 2021Quote: Wildtype genes were amplified by PCR from JE2 genomic DNA using the primers shown in table 4 and KAPA HiFi polymerase (Roche). The PCR product was cloned into the tetracycline inducible plasmid pRMC2 using KpnI and SacI restriction sites and T4 DNA ligase (NEB) ...
-
bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
-
bioRxiv - Cell Biology 2020Quote: ... Pelleted tissue was weighted and resuspended in the enzymatic digestion mix composed by 2,4 U/ml dispase II (4 ml/g of muscles) (Roche 04942078001) dissolved in Dulbecco’s phosphate buffered saline (D-PBS ...
-
bioRxiv - Microbiology 2021Quote: ... The samples were subsequently diluted by adding 150 μL of water and 4 μL was used for RT-qPCR performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
-
bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
-
bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...
-
bioRxiv - Biochemistry 2022Quote: ... Three biological replicates of sRNA from infected and noninfected MT-4 cells and cDNA samples after NAD captureSeq were measured in two technical repeats on LightCycler 480 II (Roche) by Luna Universal One-Step RT-qPCR Kit (New England BioLabs ...
-
bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
-
bioRxiv - Developmental Biology 2022Quote: ... Matrigel drops (at least 4 samples per time point) were first digested with HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml collagenase type IV (Gibco ...
-
bioRxiv - Molecular Biology 2022Quote: ... and lysed by 4 freeze/thaw cycles in the presence of 1mM PMSF and cOmplete protease inhibitor cocktail tablet (Roche). Lysates were clarified by centrifugation at 39,000xg for 1.5hrs ...
-
bioRxiv - Molecular Biology 2022Quote: ... dissociated with an equal mixture of 4 mg/ml of collagenase D and dispase II (Roche Applied Science, Indianapolis, IN) and cultured in F-12K (Corning ...
-
bioRxiv - Molecular Biology 2024Quote: ... The structure of the lacO–tetO reporter locus was checked by a set of 4 PCRs using Expand Long Template PCR System (Roche) and the following primer pairs ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
-
bioRxiv - Cell Biology 2023Quote: Cells were lysed in SDS lysis buffer (4% SDS, 20% glycerol, 125 mM Tris-HCl pH=6.8) supplemented with protease inhibitors (cOmplete ™ Mini (Roche)) ...
-
bioRxiv - Neuroscience 2023Quote: Pools of 2 to 4 organoids were resuspended in cold lysis buffer (Cell Signalling Technology) supplemented with protease and phosphatase inhibitor cocktails (Roche) and sonicated twice for 3 seconds at 10% intensity in the Branson digital Sonifier SFX 150 (Emerson) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase activity was detected in the dark in AP buffer supplemented with 45 mg/ml 4-nitrobluetetrazolium chloride (NBT, Roche) and 35 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
-
bioRxiv - Neuroscience 2024Quote: ... and stressed (n=4) DA neurons to prepare stranded RNAseq libraries following manufacturer’s recommendations using KAPA mRNA hyperprep (Roche Diagnostic). Each final library was quantified and qualified with 2200 Tapestation (Agilent) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... bmp2/4 and bmp5–8 was performed using traditional cloning methods and visualised with NBT/BCIP stock solution (Roche 11681451001), as previously described143.