Labshake search
Citations for Roche :
3051 - 3100 of 3367 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2019Quote: ... After incubation at room temperature for 4 hours in 1:2000 dilution of anti-digoxigenin antibody (Roche #11376623) prepared in TBST containing 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... in an equal volume of lysis buffer (30 mM HEPES/KOH pH 7.4, 100 mM KCl, 1.5 mM MgCl2, 0.1% Triton X-100, Protease Inhibitor Cocktail Tablets, EDTA-free, Roche Rnase inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then probed overnight at 4℃ in anti-GFP (mouse monoclonal IgG1κ, cat#: 11814460001 Millipore Sigma/Roche) diluted 1:1000 in 5% Applichem nonfat dried milk in TBS-T ...
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bioRxiv - Microbiology 2021Quote: ... The total qPCR reaction volume was 25 μl and consisted of 4 μl DNA (2,5 ng μl-1) and 12,5 μl LightCycler 480 SYBR Green I Master mix (Roche) containing 0.2 μM PCR primer (Table S5 ...
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bioRxiv - Immunology 2020Quote: ... 1 μM of each primer combination and 4 μL of Lightcycler FastStart DNA MasterPLUS SYBR Green I (Roche) in a total volume of 20 μL ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.34 mg/ml nitroblue tetrazolium (NBT) and 0.35 mg/ml 5-bromo-4-chloro-3indolyl-phosphate (BCIP; Roche), for 1–12 hours at room temperature with gentle agitation ...
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bioRxiv - Cell Biology 2019Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche). 5xSDS-PAGE loading buffer containing 25 mM dithiothreitol was added to the lysates ...
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bioRxiv - Cell Biology 2021Quote: ... was homogenized and 4 g of tissue were digested by dispase/collagenase (Collagenase: 0.1U/mL, Dispase: 0.8U/mL, Roche) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with DNAse I (4 U/ul) and 1 cOmpleteTM mini EDTA-free protease inhibitor cocktail tablet (Roche). The sample was lysed by French press at 17 KPsi (Constant Systems Ltd) ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections on slides were post-fixed at 4% PFA in 0.1M PB and treated with proteinase K (Roche). Sections were hybridized with digoxigenin (DIG)-labeled probes at 72°C overnight in hybridization solution ...
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bioRxiv - Physiology 2023Quote: ... Tissues were chopped with scissors and digested in a cocktail containing 4 units/mL LiberaseTM (Roche; Catalog# 355374) and 0.744 units/mL Elastase (Worthington Bio ...
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bioRxiv - Neuroscience 2023Quote: ... 4 % BSA in PBS) for 1 h at room temperature before being incubated with rat anti-HA (Roche) diluted either 1:250 (tsA-201cells ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl cDNA and 5 µl of LightCycler 480 SYBR Green I Master mix (Roche Diagnostics GmbH, Germany). Three technical replicates of each sample were included ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 8.5 was added and this reaction was treated with 4 Units of Alkaline Phosphatase (Roche Life Science) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Potential residual cellular phosphatase activity was blocked by incubating HRas samples for 10 min at 37 °C with PhosSTOP (Roche). HRas samples were subsequently incubated for 20 min at 37 °C with 10 mM EDTA and a 15X molar excess of either GMPPNP or GDP ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was equilibrated in hybridisation buffer for 1 hour at 68°C in pre-warmed DIG Easy Hyb solution (Roche) in a rotating hybridisation oven ...
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bioRxiv - Molecular Biology 2021Quote: ... exponentially growing cells were treated with DMSO or 1 μM PARPi for 24 h followed by 45 min incubation at 37°C with Colcemid (Roche). Metaphase preparation and aberration analysis were performed as it follows ...
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... primary antibodies were incubated at 37°C for 1hr and Discovery multimer detection system (Discovery OmniMap HRP, Discovery DAB and Purple, Roche) was used to detect and amplify immuno-signals ...
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bioRxiv - Genomics 2019Quote: ... An additional Proteinase K incubation (65 μl of 10mg/mL per 7 million cells starting material) at 65°C for two hours was followed by RNase A (Roche; 15 μl of 10mg/mL per 7 million cells starting material ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
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bioRxiv - Physiology 2019Quote: ... Tissue slices were incubated with hybridization probes for 18 h at 55°C and then incubated with sheep anti-DIG and anti-FITC antibodies conjugated with peroxidase (POD; 1:250; Roche) for probe labeling using the InSituPro VSI robot (Intavis ...
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bioRxiv - Bioengineering 2019Quote: ... For molecular cloning 3 µg of RNA were reverse transcribed for 30 min at 50°C using an oligo(dT) primer and the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) followed by 5 min enzyme inactivation at 85°C according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: To generate a peptide spanning lysin 105 and 106 the immunoprecipitates were digested in solution with 400 ng Glu-C (Sequencing Grade, Roche) at 37C for 16 hours.
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bioRxiv - Genomics 2019Quote: ... To remove RNA in the RNA:cDNA duplex the sample was incubated for one hour at 37° C with 125 mU/µl RNase H (Y9220F, Enzymatics) and 500 ng/µl RNase A (11579681001, Roche) in 1 × Rnase H buffer (Y9220F ...
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bioRxiv - Neuroscience 2019Quote: ... The suspended pellet was incubated under agitation for 30 min at 37 °C and then digested at 56°C for 30 min with 20 μl of 50 mg/ml proteinase K (315836; Roche) in 200 μl of buffer AL ...
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bioRxiv - Microbiology 2019Quote: ... The reaction was conducted in a Versant kPCR (Siemens) at 65°C for 40 minutes for BAL samples and in a Lightcycler (Roche) at 65ºC for 30 minutes for EA/BAS ...
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bioRxiv - Immunology 2021Quote: ... The reverse transcriptase was inactivated at 70 °C for 15 min and single stranded cDNA was subsequently amplified using Kapa HiFi HotStart Readymix (Roche) at a 1x concentration together with 250 nM UP-primer* (Metabion ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
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bioRxiv - Bioengineering 2020Quote: ... Tissues were finely diced manually with standard razor blades and digested for 45 min at 37°C with 1.67 Wünsch U/mL (0.5 mg/mL) Liberase TL (Roche Diagnostics, Sigma Aldrich) and 0.2 mg/mL DNase I (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Plant Biology 2021Quote: ... the blot was washed at 55°C and probe detection was performed according to the manufacturers’ protocol using the DIG luminescent detection kit (Roche). The intensity of individual bands was measured with respect to the background and integrated density value (IDV ...
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bioRxiv - Plant Biology 2021Quote: ... Northern blot analysis was performed at 50°C using protocol provided in the DIG Application Manual for Filter Hybridization (Roche). Following the hybridization ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... The eluted chromatin was then treated with RNaseA (10mg/mL) for 1 hour at 37°C and with Proteinase K (Roche) for 2 hours at 55°C ...
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bioRxiv - Cell Biology 2022Quote: mRNA was purified from ∼200 ng of total RNA with oligo-dT beads and sheared by incubation at 94°C in the presence of Mg2+ with the KAPA mRNA HyperPrep kit (Roche). Following first-strand synthesis with random primers ...
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bioRxiv - Microbiology 2022Quote: PPs were excised from the SI and digested (37 °C, 45 min) using 100 µg/ml liberase TH/DNase (Roche) in RPMI containing 5 % FCS ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was immobilized using UV crosslinking (125 mJ/cm2) and hybridized at 42°C using Abion ULTRAhyb hybridization buffer and PCR DIG probes (Roche) synthesised according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Molecular Biology 2020Quote: ... Explants were diced and then digested by sequential incubations with pronase (70 U/mL for 1 hour at 37°C; Roche) and collagenase (0.2% w/v ...
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bioRxiv - Immunology 2020Quote: The Fab fragment of antibody CR3022 was prepared by digestion of the full-length IgG using enzyme Lys-C (Roche). The digestion reaction was allowed to proceed for 2.5 hours at 37°C ...
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bioRxiv - Biochemistry 2021Quote: Mouse serum creatinine and urea levels were measured by the central laboratory of the University Hospital Cologne using a Cobas C 702 (Roche Diagnostics GmbH ...
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bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were treated for 1 hr at 55⁰C with proteinase K solution (0.4 mg/mL proteinase K (Roche; 3115828001), 50 mM EDTA ...
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bioRxiv - Immunology 2019Quote: ... LN were pierced and torn with sharp forceps in 24-well plates and incubated for 15 min at 37° C in 1 ml digestion buffer (100 U/ml collagenase A (Roche), 500 U/ml collagenase D (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 h at 37°C and protein was removed by incubation with 10 μL of proteinase K (18 mg/mL; Roche) for 2 h at 65°C ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... mice were perfused with sterile PBS and the right inferior lung lobes were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... baseline serum total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels were measured with the cobas 6000 c501 Chemistry Analyzer (Roche) to confirm hypercholesterolemia in the HFD fed rats ...