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Citations for Roche :
251 - 300 of 3378 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1× buffer (Roche Diagnostics, Mannheim, Germany), 0.6 μM of each primer and 200 μM of each deoxynucleoside triphosphate in a total volume of 25 μL ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of lysis buffer (Roche), and 40 μL of proteinase K ...
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bioRxiv - Developmental Biology 2022Quote: ... Roche Long Template Buffer 1 (Roche Applied Bioscience ...
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bioRxiv - Genetics 2020Quote: ... 400 µL Tissue Lysis Buffer (Roche) and 50 µL Proteinase K (Roche ...
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bioRxiv - Genetics 2019Quote: ... 1× FastStart PCR Reaction buffer (Roche) (50mM Tris-HCl ...
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bioRxiv - Genomics 2023Quote: ... in 1X Ligase Buffer (Roche, 10799009001)) was added to a total volume of 26 μL per tube ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... the binding buffer contained PhosStop (Roche) to prevent unwanted dephosphorylation of the peptides ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cells were lysed in Radioimmunoprecipitation assay buffer (RIPA) buffer with the addition of protease (Roche cOmplete), and phosphatase inhibitors (GB Sciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... washed for 30 min with washing buffer (1 × maleic acid buffer + 0.3% Tween 20; Roche, Catalog# 11585762001), then equilibrated for 3 min in detection buffer (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
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bioRxiv - Plant Biology 2023Quote: ... Western blotting was conducted as described above and visualized with anti HA-HRP antibody (Roche 39 #12013819001; 1:2,000).
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with rat anti-HA antibody (clone 3F10, Roche), or rabbit anti-TgSkp1 affinity purified polyclonal antibody UOK75 [76],
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bioRxiv - Molecular Biology 2022Quote: Southern blot analysis was performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and the DIG Wash and Block Buffer Set (Roche 11585762001) ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Neuroscience 2021Quote: ... containing Phosphatase (1 tablet per 3.3ml lysis buffer) and Protease (1 tablet per 16.67ml lysis buffer) inhibitor (Roche cOmplete tablets ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were washed 10 min in washing buffer and incubated 1 h in blocking buffer (both Roche #11585762001). Anti-DIG antibody was added (Roche #11093274910 - 1:1500 dilution ...
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bioRxiv - Genetics 2022Quote: ... 20-30mg of liver was homogenised in 400μl of cold homogenising buffer (50mM phosphate buffer pH 7.5 and 1x Roche EDTA-free protease inhibitor (Roche ...
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bioRxiv - Immunology 2023Quote: ... sorted cells were washed with PBS and resuspended in Antibody Buffer (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in buffer A complete (buffer A with 0.15% NP40, 0.5 mM DTT, 1 x Complete protease inhibitors, Roche), and dounced 40 times ...
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bioRxiv - Cancer Biology 2021Quote: ... (Low pH buffer, Dako; CC1m, Ventana, Roche) and endogenous peroxidase was blocked (peroxide hydrogen at 3%) ...
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bioRxiv - Cell Biology 2019Quote: ... 4 μl 5xTranscriptor RT reaction buffer (Roche), 0.5 μl RNase OUT (Fisher) ...
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bioRxiv - Genetics 2019Quote: ... Lysis buffer with protease inhibitor cocktail (Roche) was added to adjust the volume to about 1.4 ml with 120 μl taken as the input sample ...
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bioRxiv - Genomics 2019Quote: ... All buffers contained protease inhibitor (cOmplete, Roche). Inverted larvae were cross-linked at room temperature (RT ...
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bioRxiv - Cell Biology 2021Quote: ... 1mL of RBC Lysis Buffer (Roche, 11814389001) was added and tubes were incubated on a rocker at 4°C for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... treating with lysis buffer (proteinase inhibitor, Roche complete ...
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bioRxiv - Molecular Biology 2022Quote: ... All buffers contained “Complete” proteinase inhibitor (Roche) according to manufacturer’s directions.
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bioRxiv - Developmental Biology 2022Quote: ... in blocking buffer (1% blocking reagent [Roche] ...
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bioRxiv - Neuroscience 2023Quote: ... After standard pretreatment in CC1 buffer (Roche), sections were incubated with rabbit anti-HDGF antibody (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... lysed using a buffer containing protease (Roche) and phosphatase inhibitors (PhosSTOP Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Erythrocytes were removed with lysis buffer (Roche) and the remaining cell suspension filtered through a 40μm nylon mesh (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... Both buffers contained “complete” proteinase inhibitors (Roche) according to manufacturer’s directions ...
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bioRxiv - Biochemistry 2023Quote: ... and 1× HiFi reaction buffer (05917131103, Roche) supplemented with 1.875 mM MgCl2 and 3% DMSO (D8418 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then incubated overnight at 4 °C with primary antibody of interest diluted in blocking solution (Western Blotting Reagent, Roche). The appropriate species of HRP-conjugated secondary antibody (from Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against rat anti-HA clone 3F10 (Roche) or rabbit anti-TgSKP1 UOK75 [19]
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were resolved on 12% SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF Western Blotting Membranes; Roche). Membranes were blocked in PBS containing 5% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Incubation with the appropriate secondary HRP-labeled antibody was followed by detection with ECL western blotting substrate (Roche Applied Science).
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bioRxiv - Genetics 2023Quote: ... Phosphorylation of Sch9 was monitored by western blotting probed with anti-HA 3F10 antibody (1:2000; Roche Life Science, USA) followed by goat anti-rat HRP-conjugated antibody (1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were blocked and permeabilized overnight (STORM) or for 2h (STED and LSCM) (1% Roche Western Blotting Reagent (WBR), Merck ...
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bioRxiv - Systems Biology 2023Quote: ... Coverslips were washed with PBS three times for five minutes and blocked in a humid chamber for one hour at room temperature in blocking solution (1x Western Blocking reagent [Roche, 11921673001] diluted in PBS and supplemented with 0.3% Triton X-100) ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were detected using horseradish peroxidase-conjugated and detection with the Lumi- Light Plus Western Blotting Substrate (Roche). Luminescence was detected with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Developmental Biology 2019Quote: ... Probe labeling and Southern blot detection were carried out using ‘DIG High Prime DNA Labeling and Detection Starter Kit I’ (Roche). Probes were generated using PCR based amplification of the (TTAGGG ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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bioRxiv - Plant Biology 2021Quote: ... Northern blot analysis was performed at 50°C using protocol provided in the DIG Application Manual for Filter Hybridization (Roche). Following the hybridization ...
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bioRxiv - Molecular Biology 2019Quote: ... Probe labelling and detection of the blot were carried out using the DIG-DNA labelling mix and detection reagents (Anti-Digoxigenin-AP) (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Alkali-labile digoxigenin labelled deoxyuridine triphosphate (DIG-dUTP) was incorporated in Southern blot probes by PCR using PCR DIG Probe Synthesis kit (Roche) with the following specifications (refer to sequences in Supplementary table 3) ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot blotting) or electro-transferred (for Northern blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled riboprobes as previously described (22).
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bioRxiv - Molecular Biology 2023Quote: ... The Telomere Restriction Fragments (TRF) location on the blots was compared to the DNA molecular weight marker VII DIG-labeled (Roche) to obtain the average of TRF.
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bioRxiv - Microbiology 2020Quote: ... bacteria were disrupted by sonication in a lysis buffer containing 10 mM Tris-HCl pH 7.4 (buffer A) and Complete Protease Inhibitor Cocktail (Roche). Cell debris was removed by centrifugation at 16,000 g at 4°C during 10 minutes and supernatants were collected as total extracts ...
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bioRxiv - Genetics 2021Quote: Neuronal cultures were lysed in 20 µl of NP40/SDS buffer (1% Nonidet P40/0.01% SDS lysis buffer and protease inhibitors (Roche) per well ...