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Citations for Roche :
401 - 450 of 3378 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested by centrifugation and lysed by passing them twice through a French press in lysis buffer (1xPBS, 100mM NaCl, 1mM DTT, and complete EDTA-free protease inhibitor tablets (1 tablet/50mL Buffer) (Roche, Basel, Switzerland)) ...
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bioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1, LS005477] and collagenase type B [Roche, 0.35 U·ml-1, 11088807001]). The left ventricle was collected after 10 minutes’ digestion and cut into small pieces ...
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bioRxiv - Cell Biology 2021Quote: ... the pellet washed in 1x PBS then re-suspended in 1x protein loading buffer (4x NuPAGE® LDS sample buffer [Invitrogen], 1x PBS and 25 uL ß-mercaptoethanol, 2x Roche cOmplete Mini protease inhibitor cocktail tablets ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor).
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Cancer Biology 2023Quote: All experimental cells were subjected to extraction of total proteins in a lysis buffer containing a protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each tablet per 15 mL buffer, Roche, Basel, Switzerland). The proteins in cell lysates were denatured at 100°C for 10 min ...
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bioRxiv - Genetics 2023Quote: ... and ceramic beads (Precellys Lysing Kit CkMix, Precellys) in lysis buffer (Lysis Buffer 1X, Milliplex) to which a cocktail of protease inhibitors (Roche Complete Mini) and phosphatase inhibitors (PhosSTOP EASYpack ...
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bioRxiv - Microbiology 2023Quote: ... Purified PARP10 was diluted to 20 µM using MARylation buffer (SEC buffer supplemented with 0.02% Triton X-100 and 200 µM β-NAD+ (Roche, NAD100-RO)) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (Retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor). Pellets were either processed immediately or flash-frozen in liquid nitrogen and stored at -80°C ...
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bioRxiv - Cell Biology 2022Quote: ... The buffer was replaced with 1x Blocking Reagent (Roche) in PBST and the worms were incubated at least 30 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and protein extracted with ELB buffer containing PhosSTOP (Roche) and 50 μM sodium fluoride ...
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bioRxiv - Cancer Biology 2019Quote: ... 1x FastStart PCR Buffer including 2 μM MgCl2 (Roche), 0.05 mm dNTPs (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl 5X KAPA HiFi Fidelity buffer (KAPA Biosystems), 0.75 μl of each for forward and reverse primer mix (10 mM) ...
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bioRxiv - Immunology 2021Quote: ... All lysis buffers were supplemented with protease inhibitors (Roche). Cell lysates were cleared by centrifugation and protein-containing supernatants were collected into fresh tubes and quantified with the Pierce BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 40mM imidazole buffer supplemented with protease inhibitor cocktail (Roche) and 5mM β- mercaptoethanol ...
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bioRxiv - Biochemistry 2020Quote: ... both buffers containing EDTA-free protease inhibitors (cOmplete, Roche). Elution buffer (50 mM Na2HPO4 ...
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bioRxiv - Cell Biology 2021Quote: ... in buffer supplemented with complete protease inhibitor cocktail (Roche), and then eluted with excess FLAG peptide [12] ...
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bioRxiv - Biophysics 2020Quote: ... EDTA-free lysis-M buffer with protease inhibitor (Roche). Protein concentrations were determined using PierceTM BCA kit (Thermo ScientififTM) ...
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bioRxiv - Immunology 2022Quote: ... 500µL of lysis buffer containing protease inhibitors (04693159001, Roche) and phosphoStop (04906837001 ...
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bioRxiv - Immunology 2022Quote: ... 0.1% Tween 20 (TBST) buffer with proteinase inhibitor (Roche). Falcons were then vortexed for 5 min on max speed and centrifuged for 10 min at 5000 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with blocking buffer containing 3 % BSA (Roche Diagnostics), 5 % goat serum (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Genetics 2022Quote: ... and DIG Wash and Block Buffer Set (Roche 11585762001) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... This buffer was supplemented with 10 μM Leupeptin (Roche) and 1 mM Pefabloc SC (Roche) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Prehybridization was performed in DIG Easy Hyb buffer (Roche) at 40°C for 30-45 min ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated with Red Blood Cell Lysis Buffer (Roche), and washed two times with PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended in FA buffer with protease inhibitors cocktail (Roche) and frozen as drops in liquid nitrogen (N2).
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bioRxiv - Developmental Biology 2020Quote: ... blocked in blocking buffer with 2% Blocking Reagent (Roche), 20% goat serum in MABT ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... 1.4μl of End Repair & A-tailing buffer (Kapa Biosystems) and 0.6μl of End Repair & A-tailing enzyme mix (Kapa Biosystems ...
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bioRxiv - Genomics 2020Quote: ... using 1x Kapa HiFi Fidelity buffer (KK2102, Roche, UK), 1 Unit Kapa HiFi polymerase (KK2102 ...
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bioRxiv - Microbiology 2020Quote: ... Lysis and wash buffers were supplemented with PhosSTOP (Roche), cOmplete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 mL of Red Blood Cell Lysis Buffer (Roche) were added before being gently rocked for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were transferred onto 1× blocking buffer (Roche) and incubated at room temperature for 2-3 h ...
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bioRxiv - Immunology 2021Quote: ... all buffers were supplemented with cOmplete protease inhibitor (Roche). Following addition of 1 µl RNase A and incubation for 4 h at 65 °C beads were separated and supernatant was incubated with 10 µl of Proteinase K for 45 min at 50 °C ...
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bioRxiv - Genomics 2021Quote: ... 6μL PCR mix (1x KAPA HiFi PCR buffer(Roche), 0.3mM dNTPs/each(Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... (low or high pH buffer, Dako; CC1m, Ventana, Roche) and endogenous peroxidase was blocked (peroxide hydrogen at 3%) ...
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bioRxiv - Cell Biology 2023Quote: ... All buffers used contained protease inhibitor cocktail tablet (Roche) and 15 μM MG132 ...
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bioRxiv - Biochemistry 2023Quote: ... lysis buffers supplemented with 1X cOmplete Protease Inhibitor (Roche), 1 mM AEBSF (Apollo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... followed by hybridization in DIG Easy Hyb buffer (Roche) with satellite II DIG-labelled probe at 42 °C for at least 4 h ...
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bioRxiv - Genetics 2023Quote: ... (37) The buffers contained protease and phosphatase inhibitors (Roche, Cat#04693159001 and Cat#04906837001 ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% SDS sample buffer and 1x protease inhibitor (Roche). Cells were lysed in a Mini-Beadbeater-96 (BioSpec ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.55 lysis buffer containing cOmplete protease inhibitors (Roche) and universal nuclease (Pierce ...
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bioRxiv - Microbiology 2023Quote: ... 10% SDS) buffer containing 10% protease inhibitor cocktail (Roche) for 30 min on ice ...
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bioRxiv - Biochemistry 2023Quote: ... in 1× HiFi reaction buffer with MgCl2 (05917131103, Roche) were mixed with either 5’ sg RNA or 3’ sgRNA forward primer targeting the OvoA gene ...
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bioRxiv - Microbiology 2024Quote: ... All buffers contained cOmpleteTM EDTA-free protease inhibitors (Roche). Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Cells were harvested and lysed in RIPA buffer (Roche) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Membranes were blocked using DIG easy-hyb buffer (Roche) followed by incubation with 10 pmol/mL 5′/3′ DIG labeled LNA probe (Exiqon ...
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bioRxiv - Cell Biology 2019Quote: ... transferred to nitrocellulose (Hybond-ECL, GE Bioscience, Chalfont St.Giles, UK) and incubated for 1 hour in Western blocking reagent (Roche Applied Science, Basel, Switzerland). Primary antibodies (anti-human IL-17RA antibody ...
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bioRxiv - Neuroscience 2022Quote: ... 20 mg protein was loaded onto 8-10% SDS-PAGE gels for electrophoresis and subsequently transferred (90 V, 2-3 h) onto PVDF western blotting membranes (Roche, Cat. No. 3010040001) using the Criterion™ System BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... Northern blot analysis was conducted using DIG Easy Hyb and an RNA probe made with Dig RNA labeling Mixture (Roche Diagnostics, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... NK cells were then lysed in lysis buffer (IP Lysis Buffer, Pierce Biotechnology Inc., Rockford, IL) supplemented with protease inhibitors (Complete Mini, EDTA-free Cocktail tablets, Roche Holding, Basel, Switzerland) and incubated for 30 min on ice ...