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Citations for Roche :
2851 - 2900 of 8038 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: The genomic region of spe-51 was amplified by PCR from N2 genomic DNA using the Expand Long Template PCR system from Roche (Catalog Number 11681834001). The following primers were used ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Plant Biology 2020Quote: ... by standard SDS-PAGE and detection by chemiluminescence with a monoclonal Anti-GFP antibody (Mouse IgG1K, clones 7.1 and 13.1, Roche Cat. No. 11814460001) and Anti-Mouse secondary antibody HRP-conjugated (Amersham ECL GE Cat ...
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bioRxiv - Immunology 2020Quote: ... Roche Elecsys® Anti-SARS-CoV-2 Immunoassay for the qualitative detection of antibodies (including IgG) against SARS-CoV-2 was used with a COBAS e411 analyzer (disk system; Roche, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord samples specifically processed for the detection of intracellular MBP within phagocytic cells were predigested with 0.5 mg/mL Collagenase/Dispase® (Roche, #11097113001), 0.02 mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Primary and secondary antibody dilutions used for immune detection were as follows: α-HA(rat)-1:2,000 (Roche Diagnostics, Basel, Switzerland), α-GFP(mouse)-1:1500 (Roche Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from 1.0 μg of RNA using TaqMan Reverse Transcription (RT) Reagents and random hexamer primers (Roche, cat#N8080234). Equal volumes of cDNA were used for quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2019Quote: ... Blots were blocked for at least 1 hour in blocking solution at RT (5% milk in 1xTBS) before probing with primary antibody in blocking solution (α-HA-HRP, 1:2500 (Roche); α-PGB1 ...
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bioRxiv - Genetics 2019Quote: ... which was diluted 1:10 to be used in RT-qPCR carried out using LightCycler 480 SYBR Green I Master (Roche, 4707516001) under the following conditions ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...
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bioRxiv - Cancer Biology 2022Quote: Gene expression knockdown in response to shRNA expression was verified by RT-QPCR as previously described (Saurty-Seerunghen et al 2019) using the LightCycler480 (Roche, France) and the SYBR Green PCR Core Reagents kit (Bimake.com) ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR primers (Table S1 and Table S2) were designed using the Universal Probe Library Assay Design Centre (Roche Life Sciences) and primer efficiency was determined using pooled cDNA samples ...
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bioRxiv - Plant Biology 2022Quote: ... RT was performed with 5 µg of total mRNA using Transcriptor Reverse Transcriptase and oligo (dT)15 primer (Roche, Mannheim, Germany). QRT-PCR was run on a LightCycler 480 (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Then embryos were incubated overnight at RT in AP-conjugated anti-DIG antibody (Roche, 1:1000 diluted in the blocking buffer). On the third day ...
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bioRxiv - Genetics 2019Quote: ... The PCR reaction mixture in a 20 μl volume containing 10 μl of 2×SYBR Green PCR Master Mix (Roche, Mannheim, Germany, code#06402712001), 2 μl diluted reverse transcriptase product (1:100) ...
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bioRxiv - Immunology 2019Quote: ... Capture bead-bound sample pools were washed and amplified for 16 PCR cycles using KAPA HiFi Hostart ReadyMix and LM-PCR oligos (Roche Diagnostics Corp.,Indianapolis, IN). Final products were visualized on a high-sensitivity DNA Advanced Analytical Fragment Analyzer assay (Advanced Analytical Technologies Inc. ...
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bioRxiv - Immunology 2019Quote: ... after which bisulfite-converted libraries were amplified with 12 polymerase chain reaction (PCR) cycles using KAPA HiFi Hotstart Uracil+ ReadyMix and Ligation-Mediated PCR oligos (Roche Diagnostics Corp.,Indianapolis, IN). Samples submitted with less than one microgram of extracted gDNA were amplified with an additional 2 PCR cycles to increase concentration pre-capture ...
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bioRxiv - Microbiology 2020Quote: ... and final elution in 40 µL PCR-grade water (Roche). Extraction blanks were processed alongside to assess laboratory contamination ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCRs were performed using SYBR green technology (KAPA Biosystems). Data were analyzed by comparative threshold cycle (delta delta Ct ...
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bioRxiv - Cell Biology 2019Quote: ... The sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824 ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were performed using KAPA HiFi polymerase (KAPA biosystems) using the following primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... SYBR green based PCR was performed with SYBR mix (Roche). The qPCR amplification was performed using the LightCycler 480 System (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was PCR amplified with Kapa Hifi DNA polymerase (Roche), quantified using picogreen dye (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 12.5 μL 2x KAPA HiFi HotStart PCR mix (Kapa Biosystems), and 1 μL of 25 μM PE1/PE2 index primer mix ...
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bioRxiv - Microbiology 2019Quote: ... 25ng of cDNA and PCR grade water (Roche, Hertfordshire, UK). An initial denaturation cycle of 95°C for 10min was used followed by 45 cycles of 10s at 95°C ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed on a LightCycler 96 Instrument (Roche) using gene-specific and control elF4A primes (Table S5) ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative PCR was performed in the LightCycler 480 system (Roche) using primers listed in Table S1 ...
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bioRxiv - Microbiology 2019Quote: ... and hybridized with PCR-generated digoxigenin-labeled probes (Roche Diagnostics) using DIG Easy Hyb hybridization solution (Roche Diagnostics ...
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bioRxiv - Biochemistry 2019Quote: ... Expanded high fidelity PCR system (Roche Life Science, Indianapolis, IN) was used to amplify the full length PAR2 cDNA coding region ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative PCRs were performed on the LightCycler 480 System (Roche) using the KAPA SYBR FAST qPCR Kit (KAPA Biosystems ...
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bioRxiv - Pathology 2021Quote: ... qRT-PCR were performed on a LightCycler 480 instrument (Roche) using QuantiTect SYBR Green RT-PCR Kit and QuantiTect Primers (Qiagen) ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were performed using SYBR Green Master Mix (Roche). PCR conditions were as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... all PCRs were performed using HotStart ReadyMix (Kapa Biosystems #KK2601). Restriction enzymes ...
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bioRxiv - Plant Biology 2020Quote: ... amplified by PCR with KAPA HiFi HS ReadyMix (KAPA BIOSYSTEMS) and size-selected with the E-Gel size select (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was carried out using SYBR Green (Roche, Germany), and β-actin was used as control ...
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bioRxiv - Microbiology 2020Quote: ... and final elution in 40 μL PCR-grade water (Roche). Extraction blanks were processed alongside to assess laboratory contamination ...
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bioRxiv - Microbiology 2021Quote: ... or plasmid inserts were amplified with KAPA PCR (Kapa Biosystems) using full length TruSeq Illumina primers ...
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bioRxiv - Neuroscience 2021Quote: ... PCR amplification using KAPA2G Fast HotStart Genotyping Mix (Kapa Biosystems) with flanking oligos (S4 Table ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR was performed using FAST SYBR green mix (Roche) on the ABI 7500 real-time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed using a LightCycler® 480 (Roche) and TATAA SYBR® GrandMaster® Mix (TATAA Biocenter ...
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bioRxiv - Bioengineering 2022Quote: ... PCR reactions were performed using KAPA HiFi HotStart ReadyMix (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... qRT-PCR was performed using a LightCycler™ 480 (Roche) with the following parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using KAPA2G Robust HotStart ReadyMix (Kapa Biosystems) with PCR conditions as above (with the annealing temperature modified to 55°C) ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR was performed using the LightCycler 96 system (Roche) with FastStart Essential DNA Green Master (Roche) ...
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bioRxiv - Immunology 2022Quote: ... PCR reactions were performed using KAPA HiFi HotStart ReadyMix (Roche) DNA polymerase ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR amplification was performed with KAPA 2X Ready Mix (Roche), a Taq-derived enzyme with A-tailing activity ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR was run in LightCycler® 480 instrument (Roche) and results analysed by dedicated software ...
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bioRxiv - Neuroscience 2020Quote: ... on a LightCycler 480 II PCR system (Roche, Basel, Switzerland). Expression was normalized to the mean of two housekeeping genes Rps13 (Ribosomal Protein S13 ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was carried out on a LightCycler LC480 (Roche) with primer pairs designed to span exon boundaries and to generate amplicons of ~100 bp ...
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bioRxiv - Immunology 2019Quote: ... quantitative PCR (qPCR) with FastStart Essential DNA Green Master (Roche) and a LightCycler96 (Roche ...