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Citations for Roche :
2801 - 2850 of 8038 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed on a Lightcycler 480 machine (Roche) and Ct values were normalized to the housekeeping gene HPRT (hypoxanthine-guanine phosphoribosyl transferase ...
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bioRxiv - Cell Biology 2021Quote: ... PCR reaction was done by Light Cycler 2.0 (Roche). Results are presented as ΔΔCt ...
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bioRxiv - Genetics 2020Quote: ... as well as by quantitative PCR (KAPA Biosystems, USA). The sequencing libraries were clustered on a single lane of a flow cell ...
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bioRxiv - Genomics 2022Quote: ... PCR reactions were purified using 1.7×KAPAPure beads (Roche). Libraries were checked for quality and concentration using the DNA High-Sensitivity TapeStation assay (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed on a LightCycler 480 (Roche) using GoTaq qPCR Mix (Promega) ...
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bioRxiv - Genomics 2022Quote: ... Quantitative PCR was performed in a LightCycler 480 (Roche) using SYBR Green I master mix according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and concentration was determined by KAPA PCR (Roche #07960140001). Libraries were pooled together at equimolar concentrations and submitted to the Baylor Genomic and RNA Profiling Core ...
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bioRxiv - Neuroscience 2024Quote: ... For quantitative PCR the FastStart Universal Probe system (Roche) was used ...
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bioRxiv - Genomics 2024Quote: ... on a LightCycler 480 quantitative PCR (Roche, Basel, Switzerland). The sequencing was done on an Illumina MiSeq sequencer using a V2 flow cell with a 2×150 bp paired-end sequencing kit ...
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bioRxiv - Cancer Biology 2024Quote: ... or the GC-Rich PCR system (Roche, Nutley, NJ) as instructed by the manufacturer using specific annealing temperatures (Table S3) ...
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bioRxiv - Genetics 2024Quote: 12.5 Kapa HiFi HotStart PCR ReadyMix (KAPA Biosystems, KK2601)
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed using Light Cycler 2.0 (Roche). Results are expressed as ΔΔCt ...
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bioRxiv - Microbiology 2023Quote: ... These PCR fragments were amplified using PWO polymerase (Roche) or Q5 polymerase (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative PCR was performed on a LightCycler 480 (Roche) using the FastStart Universal SYBR Green Master Rox (Roche ...
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bioRxiv - Genetics 2023Quote: ... KAPA HiFi HotStart PCR Ready Mix (2×) (Roche, KK2601) was used as polymerase for all the PCR amplification steps in this paper ...
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bioRxiv - Microbiology 2023Quote: ... libraries were PCR amplified with HiFi Polymerase (Kapa Biosystems) for 12 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... amplified by the Expand High Fidelity PCR system (Roche) (primers depicted in supplementary table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was performed with KAPA HiFi HotStart ReadyMix (Roche), and amplicons were purified (QiaQuick PCR Purification Kit ...
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bioRxiv - Biochemistry 2023Quote: ... The subsequent PCR with KAPA HiFi HotStart ReadyMix (Roche) was performed using transcript-specific primers (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... and quantitative PCR was performed using SYBR Green (Roche) and a LightCycler 480 system (Roche) ...
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bioRxiv - Immunology 2023Quote: ... and amplified with HiFi HotStart PCR Mix (KAPA Biosystems). For DNA clean-up Agencourt AMPure XP beads were used (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were quantified by quantitative PCR (qPCR) (KAPA Biosystems Library Quantification Kit for Illumina platforms ...
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bioRxiv - Microbiology 2023Quote: ... in tissues was done by LightCycler PCR (Roche Diagnostics) as previously described ...
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bioRxiv - Genetics 2023Quote: ... 15 ul of 2X FastStart™ PCR Master (Roche), 2 ul of DMSO ...
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bioRxiv - Microbiology 2024Quote: ... libraries were PCR amplified with HiFi Polymerase (Kapa Biosystems) for 12 cycles ...
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bioRxiv - Immunology 2024Quote: ... PCR reactions were performed with HiFi Hotstart (KAPA Biosystems). Splint-mediated ligations were carried out with Ampligase Thermostable DNA Ligase (Epicenter Biotechnologies) ...
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bioRxiv - Plant Biology 2021Quote: ... corresponding stem-loop primers were added to the RT reaction (adapted from Yang et al., 2014) and miR823 levels were measured using Fast SYBR Green Master Mix (Roche) with miRNA823 specific forward primer and a stem-loop specific universal reverse primer ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR reactions were carried out using LightCycler® 480 SYBR Green I Master Mix (Roche #04-887-352-001) on a LightCycler® Real-Time PCR System (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 50 ng DNase-treated RNA was used as template for reverse transcription using either RT Transcriptor from Roche and random hexamers or Moloney Murine Leukemia Virus-RT (MMULV-RT ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was eluted from the beads by incubation for 1h at RT (while rotating) with 150-250 µl wash buffer supplemented with phosphatase inhibitors (PhosSTOP, Roche) and 0.5 mg/ml 3xFLAG peptide (APExBIO) ...
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bioRxiv - Microbiology 2019Quote: ... After washing with 1xPBS containing 0.1% v/v Tween-20, PIP–strips were incubated (1h, RT, agitated) with a monoclonal anti-HA antibody (clone 3F10, monoclonal antibody from Roche) at a dilution of 1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR experiments were done with SYBR Green I in a Light Cycler 96® (Roche Applied Science, Mannheim, Germany). For each primer pair (Table 4 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in 1x maleic acid for 1h at RT and riboprobes were detected by incubating in anti-digoxigenin-AP antibody (1:2000 in 1% sheep serum, Roche) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... for 1h at RT and incubated with a primary antibody diluted in blocking solution (sheep anti-digoxigenin, 1/200, Roche) overnight at +4°C in a dark humidified chamber ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... The mRNA was used in an RT reaction that was specifically selected for the barcoded mRNA (Transcriptor High Fidelity Roche). The RT product was PCR amplified and size selected using Agencourt AMPure beads (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: Validation of the RNA-Seq experiment was completed with the Universal Probe Library (UPL) RT-qPCR system (Roche Diagnostics ©). RNA samples analyzed were those used for RNAseq analyses (aphid collections from location 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Immunology 2020Quote: ... Knockdown of A3A is confirmed by RT-qPCR using allele-specific primers and SDHB probe on a Light Cycler 480 System (Roche), as previously described (Baysal et al ...
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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bioRxiv - Cell Biology 2021Quote: ... A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001, Roche) sequence detection system ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subsequently diluted by adding 150 μL of water and 4 μL was used for RT-qPCR performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Physiology 2023Quote: ... Each RT-qPCR assay was deposited in triplicates on a FrameStar® 384-well skirted qPCR plate (Roche Diagnostics, Switzerland). The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then pelleted and washed 3 times with PBS containing protease inhibitor (Roche, 11873580001). The pellets were snap-frozen and stored at -80°C for later use ...
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bioRxiv - Pathology 2020Quote: ... DAB incubation was done by diluting DAB concentrate 10 times in peroxide buffer (11718096001, Roche), after which slides were counterstained with haematoxylin for 30 seconds ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 10 times with PBSTx and the signal was developed in NBT/BCIP solution (Roche).
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed 4 times in wash buffer containing 25% formamide (Roche, 11814320001), 2x SSC (Ambion ...
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bioRxiv - Molecular Biology 2024Quote: ... The Percoll purified pellet was washed 2x times in PBS containing protease inhibitors (cOmplete, Roche) and frozen at −80 °C until used ...