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Citations for Roche :
2851 - 2900 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Input DNA was obtained following the sample phenol-chloroform purification steps post overnight de-crosslinking of 10 μg of sonicated sample at 65 °C in the presence of 0.2 M NaCl and 10 μg/μl of RNase A and 0.8 μg/μl of Proteinase K (Roche). DNA was quantified with the Qubit dsDNA HS Assay Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5 mM N-ethylmaleimide and Complete protease inhibitor cocktail (Roche, one tablet/10 ml of buffer). HA-tagged ubiquitin was purified using Pierce anti-HA magnetic beads (clone 2–2.2.14) ...
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bioRxiv - Molecular Biology 2023Quote: ... Two days after transfection cells were washed twice with ice-cold PBS and scraped in ice-cold Co-IP buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, protein inhibitor cocktail (Roche)) supplemented with 0.5% Nonident P40 ...
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bioRxiv - Molecular Biology 2023Quote: ... equilibrated in cold PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, 1X Roche cOmplete EDTA-free protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... spleens and were minced and digested in 5 mL of Iscove’s modified Dulbecco’s media (IMDM) + 10% fetal calf serum (FCS) (cIMDM) with 250 μg/mL collagenase B (Roche) and 30 U/mL DNaseI (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were spun down at 500 g for 2 min and washed three times with SpyTtag-Wash buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 1x complete protease inhibitor (Roche)) and resuspended as a 50% slurry in wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Triton X-100 (0.1% ...
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bioRxiv - Genomics 2023Quote: ... 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1mM DTT, 1x EDTA-free protease inhibitor cocktail [Roche]) was added to the tubes containing thawed parasites and incubated on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... pH 6.8, 10 mM DTT, 1 mM EDTA, 0.1% Tween, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche). The samples were centrifuged at 3000g for 6 min at 4°C using a tabletop centrifuge ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Then was added Triton X-100 at a final concentration of 0.1% and the cells were incubated for 5 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was removed and the cell pellet washed in lysis buffer 2 (200 mM NaCl, 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.5 mM EGTA, cOmplete protease inhibitor by Roche). Cells were taken up in sonication buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM MgCl2, 0.1 M EDTA, 10 % glycerol and 0.5 mM DTT, supplemented with a protease inhibitor cocktail from Roche) or Trap buffer (10 mM HEPES pH = 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 min) and the cell pellet was resuspended in 1 ml vPBS containing EDTA-free protease inhibitor (Roche). The cells were pelleted again by centrifugation (750 x g ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear pellets were resuspended in shearing buffer (0.1% SDS, 1 mM EDTA, 10 mM Tris-HCl pH 7.5, 1X Roche protease inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were lysed in HEPES buffer (50 mM HEPES pH 7.5, 800 mM KCL, 10% glycerol, 0.2 mM PMSF, 1x cOmplete ETDA- free [Roche]) including 10 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg/mL DNase I) containing a single dissolved cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche: 11873580001). The mixture was first gently homogenized with a Biospec Tissue Tearor (Dremel ...
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bioRxiv - Biochemistry 2023Quote: ... 10% glycerol and 0.1% NP-40 supplemented with 0.1 mg of lysozyme/ml and complete inhibitor cocktail (Roche)) and sonicated to disrupt the cells ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing chromatin fragments was then treated with 6-10 µg/g cells DNase-free RNase (Roche) at 37°C for 30 minutes and subsequently cleared by centrifugation at 30,000 rcf for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in a HBSS solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Physiology 2024Quote: ... samples were diluted using buffer D (20mM HEPES, pH7.9, 10% Glycerol, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10mM NaF, Roche PhosSTOP ...
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bioRxiv - Cancer Biology 2024Quote: ... protein samples were subjected to 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Roche, Mannheim, Germany). Afterwards ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
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bioRxiv - Plant Biology 2024Quote: ... supplemented with 1 tablet of protease inhibitor cocktail per 10 ml buffer (cOmplete™ Proteasehemmer-Cocktail, ©Roche) with an ultrasonic homogenizer (Hielscher Ultrasonics UP200St ...
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bioRxiv - Developmental Biology 2024Quote: ... harvested and lysed 14 h later in 300 µl binding buffer (20 mM HEPES pH 7.6, 150 mM MgCl2, 10% glycerol, 0.05% NP-40, 1 mM DTT, ROCHE cOmpleteTM ULTRA-tablet Mini protease inhibitor) ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were washed 4x in Co-IP wash buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.05% Triton-X100, Roche Complete Protease Inhibitor cocktail (1 tablet/50 mL) ...
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bioRxiv - Molecular Biology 2024Quote: ... then Lysis Buffer 2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Roche cOmplete™ ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was diluted 10-fold with ChIP incubation buffer (70 mM NaCl, 10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 2 mM EDTA, 0.1% Triton, 1 x Roche Complete EDTA-free Protease inhibitor cocktail) ...
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bioRxiv - Microbiology 2024Quote: ... 500mL of Buffer 1 (20mM KHEPES pH 7.9, 50mM KCl, 10% Glycerol) with a protease inhibitor tablet (Roche) was added and samples were subject to ultrasonication in a cup horn sonifier (Branson ...
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bioRxiv - Microbiology 2020Quote: ... and further purified using the High Pure RNA Isolation kit (Roche). RNA quantity was assessed using the Quant-iT RiboGreen RNA Assay kit (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were constructed using the KAPA HyperPrep Kit (Kapa Biosystems, Switzerland) followed by sequencing on an Illumina NovaSeq6000 with 2×150 bp paired-ends.
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bioRxiv - Molecular Biology 2020Quote: ... Fibroblast proliferation was determined using colorimetric BrdU incorporation assay kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Assays were performed using the Luciferase Reporter Gene Assay Kit (Roche) to obtain data as relative light units (RLU) ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions with KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) were setup with 80ng genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... Exome libraries were prepared using a Hyper Prep Kit (KAPA Biosystems), pre-capture PCR and SureSelectXT Human All Exon V5+UTR baits (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was isolated using a KAPA Mouse Genotyping Kit (Roche). PCR amplification of the N-terminal region of TRIM1 was performed using Q5 High-Fidelity 2X Master Mix (New England BioLabs ...
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bioRxiv - Developmental Biology 2021Quote: ... The In Situ Cell Death Kit (TMR red; Roche Applied Science) was applied to fixed retinal sections as per the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Library concentration was determined using a KAPA Library Quantification Kit (Roche). Deep sequencing was performed on an Illumina NovaSeq 6000.
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bioRxiv - Genomics 2022Quote: ... two libraries were constructed using the Kapa Hyper Prep kit (Roche) according to Arima’s recommendation (Library Preparation using KAPA Hyper Prep Kit DOC A160108 v01) ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP library was constructed by KAPA Hyper Prep Kit (KAPA BIOSYSTEMS) and SeqCap Adapter Kit A (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared using the KAPA Library Preparation Kit (KAPA Biosystems), and were amplified for a total of 12 PCR cycles (15 s at 98°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and quantification performed with a KAPA Library Quantification Kit (Roche, KK4844). Libraries were sequenced by the Babraham Institute Next Generation Sequencing Facility on an Illumina HiSeq2500 in Rapid Run 50bp Single End mode.
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bioRxiv - Genomics 2020Quote: ... with KAPA Library Quantification Kit (Kapa Biosystems Inc., Wilmington, MA, USA) for concentration ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed using the KAPA Hyper Prep Kit (Roche) or NEBNext UltraII DNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Physiology 2019Quote: ... and the DISCOVERY ChromoMab DAB kit (RUO) (Ref. 760-159, Roche). Sections were incubated with the primary antibody anti-Pecam1 (Ref ...
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bioRxiv - Genomics 2019Quote: ... and quantity measured with KAPA Library Quantification Kits (KK4835, KAPA Biosystems).
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bioRxiv - Genomics 2019Quote: ... and quantity measured with KAPA Library Quantification Kits (KK4835, KAPA Biosystems)
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bioRxiv - Physiology 2019Quote: RNA was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Physiology 2019Quote: ... RNA was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Genomics 2019Quote: ... and quantified with the KAPA Library Quantification Kit (Kapa Biosystems #KK4844), and then sequenced in a paired-ended manner using the NextSeq 500 (Illumina ...