Labshake search
Citations for Roche :
2801 - 2850 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL DNA (10 ng/μL) were amplified using Lightcycler® FastStart DNA Master HybProbe Mix (Roche, Germany) and the respective LightSNiP Assay (TIBMolBiol ...
-
bioRxiv - Immunology 2020Quote: ... The remaining tissue was incubated in digestion buffer (RPMI 1640, 10% FCS, 1.25 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma– Aldrich) ...
-
bioRxiv - Developmental Biology 2021Quote: ... washing the pellet in PBS and resuspending the cells in ice-cold 10 ml buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1% NP-40, c0mplete Mini Protease inhibitor EDTA free from Roche) and rotating for 10 min at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2, 0.5% Triton X-100, 0.25% Nonidet P-40 and 10% glycerol, supplemented with protease inhibitors [Roche]). After a 10 minute incubation on ice ...
-
bioRxiv - Pathology 2021Quote: ... were isotonically lysed using a dounce homogenizer in 0.25 ml of 10 mM Tris Maleate (pH 7.0) buffer plus protease inhibitors (Complete inhibitors from Roche). Samples were centrifuged at 8,000 x g and the protein concentration of the supernatants were determined by Bradford assay ...
-
bioRxiv - Plant Biology 2019Quote: ... Soluble proteins were extracted using 50 μl of protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT and 1×Complete protease inhibitors [Roche]), incubated on ice for 5 min ...
-
bioRxiv - Cell Biology 2019Quote: Whole-cell lysates were obtained by incubating cells in lysis buffer (1% Triton X-100, 50mM HEPES pH 7.4, 140mM NaCl, 1.5mM MgCl2, 1mM EGTA, 10% glycerol; supplemented with Roche protease/phosphatase inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2019Quote: ... and then slides were washed with PBS and incubated for 10 min with OptiView HQ Universal Linker (Roche) at RT ...
-
Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... BMDMs were activated overnight with 0.5 μg/mL lipopolysaccharide (LPS, MilliporeSigma) and 10 ng/mL IFN-γ (Roche) for M1 polarization ...
-
bioRxiv - Biochemistry 2020Quote: ... 250 mM imidazole) and incubated after addition of 1 or 10 mM phenylmethanesulfonyl fluoride (PMSF) (Roche, Basel, Switzerland), or 1 mM H2O2 (Sigma Aldrich ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... at 10 µg/ml for 40 minutes at 37° C before applying the TUNEL reaction mix (Roche Inc.) for 90 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Supernatant was separated from lysates by centrifugation at 20,000xg for 10 minutes and used for immunoprecipitation using 20μg of anti- GFP antibody (Roche) coupled to 50μl of Protein G Dynabeads (Life Technologies ...
-
bioRxiv - Genomics 2021Quote: Crosslinked MEFs per protocol above from 6-well dish were washed once in 2 mL PBS and collected in 1 mL cold NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 1 mM MgCl2, 1 mM DTT, 0.1% Triton X-100, Roche Protease Inhibitor Cocktail (Millipore (Burlington ...
-
bioRxiv - Molecular Biology 2021Quote: ... Nuclei isolated from approximately 2.5x107 cells were resuspended in one ml of extraction buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM DTT, 0.25%NP-40, 1x Roche Complete protease inhibitors ...
-
bioRxiv - Cancer Biology 2020Quote: ... containing 10 ml of DMEM media and 1 mg/ml collagenase D (Sigma-Aldrich, COLLD-RO Roche, #11088866001). The tubes were placed on a gentleMACS Dissociator (MACS Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2020Quote: ... and pellets underwent a second lysis in high salt buffer (25mM HEPES-KCl pH7.9, 350mM NaCl, 10% w/v sucrose, 0.01% NP-40, 1X protease inhibitor cocktail from Roche, 20mM N-ethylmaleimide ...
-
bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
-
bioRxiv - Immunology 2021Quote: ... 100 µL of saliva sample was mixed with 2 µL of 10 U/µL α2-3,6,8-Neuraminidase (Sialidase) from Arthrobacter ureafaciens (Roche) and reacted at 37°C for 30 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... wild-type or transgenic animals in an equal volume of Pedro’s buffer (30mM HEPES, pH 7.5, 100mM KOac, 10mM EDTA, 10% glycerol, 1 mM DTT, 1x Roche complete mini protease inhibitors ...
-
bioRxiv - Genetics 2020Quote: ... with fetal bovine serum (FBS, 10% v/v, Biowest) and Penicillin/Streptomycin (100 U/mL, Roche Diagnostics GmbH). Cells were cultured on 10 cm sterile plates in 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... 10% (v/v) glycerol and 0.007% (w/v) bromophenol blue] including 50 mM DTT and protease inhibitor (Roche) and then boiled (95ºC ...
-
bioRxiv - Cell Biology 2022Quote: ... Beads were incubated with immature and mature DC lysates prepared in RIPA buffer (10 mM HEPES, 50 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, protease inhibitor cocktail (Roche), 1 mM Na3VO4,1 mM DTT ...
-
bioRxiv - Cell Biology 2022Quote: ... Twenty uL qPCR reactions were prepared with 10 uL of the KAPA SYBR FAST Universal MasterMix (Roche, KK4602), 5 uL cDNA (representing 5 - 20 ng RNA per reaction) ...
-
bioRxiv - Molecular Biology 2022Quote: Harvested cells were resuspended in 50 mL of lysis buffer (25mM HEPES 7.9, 150mM NaCl, 2.5mM MgCl2, 10%glycerol, 1X cOmplete EDTA-free protease inhibitor (Roche)) and sonicated for a total processing time of 5 minutes at 40% power ...
-
bioRxiv - Molecular Biology 2022Quote: ... A total of 20 µL reaction mixture was prepared comprising of 10 µL KAPA HiFi HotStart ReadyMix (Roche); 1 µL forward and reverse primers (5 pmol each) ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal quantities of protein were electrophoresed on 10% SDS-PAGE and transferred onto a nitrocellulose membrane (Roche Diagnostics). The membranes were incubated with the primary antibody overnight at 4°C after blocking with 5% milk dissolved in TBST buffer for another 2 h at room temperature (25°C) ...
-
bioRxiv - Plant Biology 2022Quote: ... Slides were washed 10 min in washing buffer and incubated 1 h in blocking buffer (both Roche #11585762001). Anti-DIG antibody was added (Roche #11093274910 - 1:1500 dilution ...
-
bioRxiv - Microbiology 2022Quote: ... cells were lysed at 4°C in 1 mL lysis buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.1% IGEPAL CA-630, EDTA-free protease inhibitor cocktail [Roche]). Protein concentration in the lysates was quantified by BCA assay (ThermoFisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5mL cold lysis buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; 1x complete protease inhibitor; 11836145001 Roche) for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... cells were washed 2x with ice-cold PBS and then incubated for 10 min on ice with swelling buffer (10mM Tris-HCl [pH 7.5], 10% glycerol, 3mM CaCl2, 2mM, MgCl22, 0.5mM DTT, protease inhibitors [Roche] and 4 U/ml RNase inhibitors [RNaseOUT ...
-
bioRxiv - Physiology 2022Quote: ... the muscles were digested in a solution of 10 g/L Collagenase B (11088831001 Roche Canada, Basel, Switzerland) and 4 g/L Dispase II (D4693 Millipore Sigma ...
-
bioRxiv - Microbiology 2022Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 x g for 10 min at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... the chromatin pellet was suspended with buffer B (20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 10 mM EDTA, and 1× complete protease inhibitor cocktail; Roche), and then mononucleosome was extracted ...
-
bioRxiv - Cell Biology 2022Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Triton X-100 (0.1% ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 min) and the cell pellet was resuspended in 1 ml vPBS containing EDTA-free protease inhibitor (Roche). The cells were pelleted again by centrifugation (750 x g ...
-
bioRxiv - Physiology 2024Quote: ... from two bees were pooled and homogenized in 250 µL of phosphate-buffered saline (7 mM NaCl, 2.7 mM KCl, and 10 mM PO4, pH 7.4) containing cOmplete protease inhibitors (Roche) at kept on ice until further processing ...
-
bioRxiv - Molecular Biology 2024Quote: ... primers and template in 10 μl of nuclease-free water were processed with LightCycler 480 Instrument II (Roche) as follows ...
-
bioRxiv - Developmental Biology 2024Quote: ... Nuclei were washed once in 1 mL Wash350 (20 mM HEPES pH 7.5, 350 mM NaCl, 10 mM NaButyrate, 0.025% Digitonin, protease inhibitor cocktail (Roche), 0.5 mM Sper-midine ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated in a HBSS solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... The purified parasites were lysed in 200 μl RIPA buffer (150mM NaCl, 10 mM TrisHCl, pH 7.5, 0.1% SDS, 1% TX100 containing 2x protease inhibitor cocktail (Roche), and 1 mM PMSF ...
-
bioRxiv - Immunology 2024Quote: ... Dissected meninges were incubated in digestion medium (RPMI/10% FBS/80μg/mL DNase I/40μg/mL Liberase TM (Roche)) ...
-
bioRxiv - Immunology 2024Quote: ... and gently digested at 4C in RPMI+10% FBS containing 100mg/mL soy trypsin inhibitor (Roche, cat #10109886001), 250U DNase I (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysis buffer (50 mM Tris-HCl, pH 7.5; 500 mM NaCl; 10 mM imidazole; 0.5 mM TCEP; 1X Roche cOmplete protease inhibitors ...
-
bioRxiv - Systems Biology 2023Quote: ... Cell pellets were resuspended in 400µL of ice-cold disruption buffer (PBS containing 10% Glycerol, 1mM MgSO4, Benzonase 0,05U/m, Roche complete cocktail EDTA free 1 tablet/10mL ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 mM EDTA) with the addition of 0.5% SDS and 200 μg/ml of RNase A (Roche, 10109169001). The samples were heated at 37 °C for 60 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were then resuspended in sonication buffer (50 mM Tris-HCl pH 8.1; 10 mm EDTA; 1% Triton-X; 0,1% deoxycholate sodium; 100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) and sonicated 6 times with 2 min cycles (Branson Sonifier 250 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then lysed and harvested in ChIP lysis buffer (50 mM Tris-HCl pH 8.1; 0.5% SDS; 10 mm EDTA;100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) by centrifugation for 6 min at 2,000 × g ...
-
bioRxiv - Microbiology 2023Quote: ... any contaminating DNA was removed by treating 25 μg of RNA with 10 U of DNase I (Roche) for 20 min and purified as previously described (Adams et al. ...