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Citations for Roche :
2751 - 2800 of 7428 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... using the KAPA HiFi ReadyMix PCR kit (Roche, Basel, Switzerland) with TKO outer forward and reverse primers (76) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA probes were synthesized using DIG-RNA labeling kit (Roche) [70] ...
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bioRxiv - Cancer Biology 2024Quote: ... library was prepared using KAPA HTP library kit (KAPA biosystems). ChIP libraries were sequenced to obtain 30-40 M reads for each sample on HiSeq 2500 or 4000 with either SR50 or PE50.
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using High Pure RNA Isolation Kit (Roche) according to manufacturer’s instructions and quantified with Nanodrop 2000 Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... were quantified using the KAPA Library Quantification Kit (KAPA Biosystems). Pooled libraries were sequenced by an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2 ...
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bioRxiv - Genomics 2020Quote: ... Note that this exome capture kit generates much less discordantly mapped read pairs than the kit from Roche (2 versus 22, compare with Figure 2). Accordingly ...
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bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Pellets were resuspended in nuclear lysis buffer (20mM HEPES pH 7.9, 0.4M NaCl, 1mM EDTA, 10% Glycerol, Protease Inhibitor (1X) (Roche)) and sonicated at amplitude 40 for 10 seconds twice ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA, 300 mM NaCl, 10 mM Tris.HCl [pH 8.0], 1 mM EDTA, 1x Complete protease inhibitor [Roche] ...
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bioRxiv - Biophysics 2022Quote: ... 300 mM NaCl and 10 mM imidazole pH 8.0) containing one tablet of cOmplete protease inhibitor cocktail (Roche), and lysed by sonication ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM MgCl2, 10 % v/v glycerol, 140 mM NaCl, 0.1 mM EDTA, 0.5 % Tween20, 1 mM DTT and Roche cOmplete-Ultra EDTA free protease inhibitor) ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected hippocampi were dissolved in lysis buffer (1M Tris-Cl, pH 7.5; 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP40 and 10 mM Tris (pH 7.5)) supplemented with cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland). Samples were incubated on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The brain tissue was then homogenized on ice using a dounce homogenizer and diluted to 5 mL/g in homogenization buffer with RNase inhibitors (10mM Tris-HCl pH7.4, 0.8M NaCl, 1mM EGTA, 10% sucrose, 1X Roche protease inhibitor ...
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bioRxiv - Developmental Biology 2019Quote: ... third-instar larvae were dissected in PBS and incubated with 5-bromo-2’-deoxy-uridine (10 μM, Roche) for 45 min ...
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bioRxiv - Microbiology 2019Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 × g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of 2× SYBR Green Real-time PCR Master Mix (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Biophysics 2019Quote: ... 10% glycerol) supplemented with 2mM β-mercaptoethanol and cOmplete EDTA-free protease inhibitor tablets (1tablet/50mL, Roche Diagnostics) for 15min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... then immersed in 0.1 M maleate buffer (pH 7.5) for 10 minutes before blocking in 1% Blocking Reagent (Roche) for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed by the addition of 1000 μL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.0, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice to generate an average DNA fragment size of ∼250 bp ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi were collected 24 h after KA treatment and protein extracts were obtained by processing samples with lysis buffer (Tris-Base 0.5 M, pH 7.4; SDS 10 %) containing phosphatase and proteinase inhibitors (Roche). Lysates were clarified by centrifugation at 14000 x g for 10 min and protein concentration was measured using the bicinchoninic acid assay ...
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bioRxiv - Molecular Biology 2020Quote: ... 110 mM KOAc, 0.5% Triton X-100, 0.1% Tween 20, 10 mM MnCl2, 1x proteinase inhibitors (complete, EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.9; 10 mM KCl; 0.1% Triton X-100; 20% Glycerol; 0.5mM spermidine; 1x complete protease inhibitor [Roche # 11836170001]), collecting by centrifugation (600 g ...
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bioRxiv - Neuroscience 2021Quote: Real-time PCR was carried out in a 10 μL reaction using SYBR Green PCR Master Mix (Roche) using 1ng/μL of cDNA according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM EDTA) supplemented with protease inhibitors (complete mini EDTA-free, Roche, 1 tablet / 50 mL lysis buffer). The lysed neurons were mixed with 4X Laemmli sample buffer and heated at 70°C for 10 minutes (stored at −20°C until use) ...
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bioRxiv - Plant Biology 2021Quote: Root tissue was ground in IP buffer (50mM Tris-HCL, pH7.5, 150mM NaCL, 10% glycerol, 0.1% NP40) containing cOmplete protease inhibitor (Roche). After clearing lysates at 12,000x g (10 min) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 * 107 cells were pelleted and resuspended in 1 ml of lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) including 0.25x protease inhibitor (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 1 ml buffer (50 mM Tris, pH 8.0, 10 mM DTT, 0.1% SDS, 100 μM PMSF, phos-stop tablet [Roche]), and cells were lysed by 2 minutes of vortexing with zirconia beads (BioSpec Products) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded in 10 cm tissue-culture treated dishes and transfected with FuGENE 6® reagent (Roche) for 24 hr ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μl 1xTaqMan Gene Expression Assay primer and 10 μl 1x FastStart Universal Probe Master-mix (Roche, 04913957001) were applied ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were blocked with 10 mL of 1x blocking solution diluted in 1x Maleic Acid Buffer (Roche, 115857262001) with 0.3% TWEEN 20 for 30 minutes at room temperature ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: ... The cell pellet was resuspended in 1 mL ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-Cl, pH 8.0, and 0.25 x protease inhibitor cocktail [Roche]) and transferred to Beckman Coulter 4mL Polycarbonate Thick Wall Centrifuge Tubes (13 × 64 mm) ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: ... Cell pellet was resuspended in 1 mL ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-Cl, pH 8.0, and 0.25 x protease inhibitor cocktail [Roche]) and transferred to Beckman Coulter 4mL Polycarbonate Thick Wall Centrifuge Tubes (13 × 64 mm) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted then washed once with 250 uL TAP extraction buffer (40 mM HEPES pH 7.5, 10% Glycerol, 350 mM NaCl, 0.1% Tween-20, phosphatase inhibitor cocktail and proteinase inhibitor cocktail from Roche). Cell pellets were resuspended in 180 uL modified 2X SDS buffer then boiled at 100 °C for 4-5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting pure nuclear pellet was first resuspended with 70 μL low salt buffer (20 mM HEPES, pH 7.6, 1.5 mM MgCl2, 10 mM KCl, 25% glycerol, protease inhibitor (Roche)) and 2.5 μL benzonase (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... and 10% glycerol] containing the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche). The lysate was frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Neuroscience 2022Quote: ... and cells were dissociated by 10 passages through a glass pipette in the presence of 25U DNase (Roche). Cells were then collected by centrifugation for 5 min at 500xg ...
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bioRxiv - Genomics 2022Quote: ... twice 10 min with cold TET buffer (10 mM Tris/HCl pH7.5, 1 mM EDTA, 0.1% Tween-20, protease inhibitor cocktail (Roche)) and eluted with TE buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 1 M NaCl and protease inhibitor cocktail (cOmplete, Mini, Roche; two tablets per 10 mL of lysis buffer). The lysate was sonicated before AHA-labeled proteins were covalently coupled to alkyne-tagged agarose beads using reagents provided in the kit ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Tris, pH 7.5, 1 mM EDTA, 0.5% Nonidet P-40, protease and phosphatase inhibitor cocktail [Roche]). Cell lysates were denatured in Laemmli sample buffer and incubated for 5 min at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were blocked in Blocking Buffer (5% sheep serum with 10% Roche Blocking Reagent 10x in MABT) for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000 ...
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bioRxiv - Microbiology 2022Quote: ... were incubated at 4°C with hypotonic lysis buffer (10 mM Tris–HCl pH 8.0, 1 mM KCl, 1.5 mM MgCl2, with protease inhibitors (ROCHE)) for 2 hours while rotating ...
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bioRxiv - Plant Biology 2022Quote: ... Cell pellets were thawed on ice and resuspended in lysis buffer (20 mM sodium phosphate pH 6.8, 10 mM DTT, 1 mM EDTA, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice and centrifuged for 10 min at 500 g and 4 °C to remove intact cells and cell debris ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was homogenized in 200μl Tris buffer (pH 7.4) containing 10% sucrose and protease inhibitors (Complete; Roche Diagnostics), sonicated and kept at −80°C until use ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was diluted 1:10 before usage and amplified on a LightCycler 480 (Roche Diagnostics, Basel, Switzerland) using the Applied Biosystems SYBR Green Master Mix (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were resuspended in wash buffer [(10 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA pH 8.0, protease inhibitor (Roche)] and rotated for 10 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3.4 mM KCl,10 mM CaCl2, 2.4 mM NaHcO3, 2 mM HEPES, pH7.0 and a protease inhibitor tablet [Roche]). The cell suspension was incubated at 4 °C for 15 mins with rigorous end-over-end rotation ...