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Citations for Roche :
2601 - 2650 of 8038 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms ...
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bioRxiv - Molecular Biology 2020Quote: The Expand Long Template PCR system (Roche) with forward and reverse primers at nt 2,478–2,512 and nt 1,933–1,906 ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCRs were conducted with SybrGreen (Roche) on 384-well plates using a LightCycler 480 (Roche ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed using FastStart polymerase (Roche, 047384200010 under the following conditions ...
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bioRxiv - Genetics 2019Quote: ... A PCR reaction using KAPA polymerase (Roche), 10 ng template ...
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bioRxiv - Neuroscience 2021Quote: ... PCR-grade Proteinase K (Roche, Cat#03115828001) was added and incubated at 45°C and 650 rpm for another hour ...
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bioRxiv - Bioengineering 2022Quote: ... Quantitative PCR was performed with LightCyclerÒ480 (Roche) using TaqMan® Gene Expression Master Mix (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... with SYBR Green PCR Master Mix (Roche) using validated primers specific to each target each gene ...
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bioRxiv - Cell Biology 2022Quote: ... and LightCycler 480 quantitative PCR machine (Roche Diagnostics International AG ...
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bioRxiv - Pathology 2022Quote: ... on a PCR System (Roche, Basel, Switzerland). The PCR primers are listed in Table 1 ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification (Kapa Hot Start polymerase, Roche) produced a pooled library of amplicons with small overhangs homologous to a pUC19 plasmid containing the CD19 CAR HDR sequence ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... for PCRs KAPA HiFi HS RM (Roche) and different primer sets spanning regions out and/or inside expected deletions were used ...
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bioRxiv - Immunology 2023Quote: ... quantified by quantitative PCR (KK4824, KAPA Biosystems) and then sequenced on illumina NovaSeq 6000 with PE150 read length.
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bioRxiv - Cancer Biology 2023Quote: ... SYBR Green PCR master mix (Kapa Biosystems) was used for quantitative PCR in triplicate reactions with primers listed in Table S9 ...
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bioRxiv - Molecular Biology 2023Quote: ... Expand™ Long Template PCR System (Roche) was used with the following thermocycling program ...
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bioRxiv - Neuroscience 2024Quote: ... using SYBR Green PCR Master Mix (Roche) according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems).
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Neuroscience 2020Quote: ... SYBR green quantitative PCR (qRT-PCR) was performed using the LightCycler® 480 system (Software 3.5, Roche Molecular Biochemicals). To determine the relative concentration of mRNA expression ...
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bioRxiv - Molecular Biology 2023Quote: ... V3-V4 regions of 16S rRNA genes were amplified by PCR (first stage of PCR) using 12.5µL of 2X KAPA HiFi HotStart Ready Mix (Roche, France), 5µL of amplicon PCR Forward primer 1 µM (5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG –3’) ...
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bioRxiv - Cell Biology 2020Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with HRP-conjugated rabbit anti-sheep antibodies and detection using ECL reagents (Roche Diagnostic GmbH). A series of timed exposures were undertaken to ensure that densitometric analyses were performed at exposures within the linear range ...
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bioRxiv - Cell Biology 2019Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-DIG antibody conjugated to alkaline phosphatase allowed detection of hybridized riboprobes according to the manufacturer’s instructions (Roche).
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bioRxiv - Neuroscience 2022Quote: ... Staining of tissue and RNA detection was automated using a Discovery Ultra IHC/ISH staining platform (Roche Diagnostics). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Detection of DIG-labeled probe was performed with a fluorescein-conjugated sheep anti-DIG antibody (1:250, Roche). All buffers were made with RNase-free PBS or water.
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was tested for lactate dehydrogenase using the Cytotoxicity Detection KitPLUS (LDH) (Roche, Indianapolis, IN, USA).
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bioRxiv - Immunology 2019Quote: ... RT-qPCR was performed in triplicates using the one-step system Lightcycler 480 RNA Master Hydrolysis Probes (Roche), with the following PCR profile ...
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bioRxiv - Synthetic Biology 2021Quote: ... qPCR of the RT cDNA was performed according to the manufacturer’s instructions in a LightCycler 480 II (Roche Life Science using SYBR GREEN (Roche Life Science ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using SYBR Green qPCR Master Mix (TOYOBO, QST-100) in the LightCycler480 system (Roche). The constitutively expressed tubulin gene (MGG_00604 ...
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bioRxiv - Cell Biology 2024Quote: ... and the fixed cells were then resuspended in 1 ml RT vPBS containing EDTA-free protease inhibitor (Roche). The cells were attached to the coverslips by centrifugation (1000 x g ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed according to protocols provided by the manufacturer using FastStart Essential DNA Green Master (Roche) and LightCycler96 (Roche ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed three times with NBT/BCIP (Roche Diagnostics, Mannheim, Germany) before being incubated with alkaline phosphatase-labeled anti-rabbit IgG (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: The different CRE candidate sequences were amplified using PCR-specific primers (see Table S2) and the Expand High Fidelity PCR system (Roche). Amplified fragments were gel-purified and cloned into the pSK-lacZ vector as in (Beccari et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Primers were generated by www.leishgedit.net (Table S5) and all PCR reactions were performed using Expand High Fidelity PCR System (Roche, Basel, Switzerland), pooled and purified using PCR purification kits ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was performed using 96-well white PCR plates using the LightCycler® 480 II system (Roche Applied Science, Germany). The amplification started with an initial denaturation step at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: Four consecutive and non-overlapping fragments from the pSP64 HPV16 bacterial artificial chromosome were PCR amplified using the Expand High Fidelity PCR system (Roche). This resulted in complete coverage of the HPV16 genome ...
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bioRxiv - Neuroscience 2022Quote: ... The attB-flanked hFGD4 fragment was produced by PCR using the “Expand High Fidelity Plus PCR System” (#3300226001, Roche, Switzerland) and the following primers ...
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bioRxiv - Systems Biology 2022Quote: ... PCR was immediately performed by adding 6ul of PCR mix to each well (2ul Kapa HiFi Hotstart buffer (5x, Roche), dNTPs 0.12ul (25mM/each ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We generated standard PCR-free Illumina paired-end sequencing libraries using KAPA Hyper PCR-free library reagents (KK8505, KAPA Biosystems) in Beckman robotic workstations (Biomek FX and FXp models) ...
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bioRxiv - Genetics 2019Quote: ... The PCR reactions were performed on the Access Array System (Fluidigm) using KAPA2G 5X Fast Multiplex PCR Mix (Kapa Biosystems). Barcodes were added in a second round of PCR using Phusion DNA polymerase (NEB cat ...
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bioRxiv - Cancer Biology 2020Quote: A unique Fluidigm barcode was added to each sample by PCR in 10 μl reactions using the Fast Start High Fidelity PCR System (Roche) containing ...
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bioRxiv - Biochemistry 2019Quote: ... the collected cDNA solution was used for qRT-PCR using Power SYBR® Green PCR Master Mix (Roche, Indianapolis, USA) according to the schemes of the manufacturer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each of these pools was amplified in a 50 μl PCR reaction using Kapa HiFi HotStart PCR Mix (Kapa Biosystems), 40 ng of synthesized oligonucleotide as template and pool-specific primers ...
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bioRxiv - Genomics 2021Quote: ... The oligonucleotides were used as PCR templates and amplified by six rounds of PCR using KAPA HiFi polymerase (Kapa Biosystems) and primers F_N50_lib and R_N50_lib ...
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bioRxiv - Cell Biology 2020Quote: Global amplification of the cDNA by PCR was then performed by adding PCR mastermix consisting of: 12.5ul 2x KAPA HiFi HotStart ReadyMix (KAPA Biosystems), 0.25ul 10mM Smart PCR primer ...
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bioRxiv - Genomics 2021Quote: ... PCR products from the 5 reactions were mixed for library preparation using FastStart High Fidelity PCR System dNTPack (Roche, 3553400001) and Nextera XT Index Kit (illumina ...