Labshake search
Citations for Roche :
201 - 250 of 1071 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Ultrathin sections (∼100 nm) were labelled using mouse anti-GFP antiserum (1:300, Roche 11814460001) and 18 nm Colloidal Gold AffiniPure Goat Anti-Mouse (Jackson ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were subsequently fixed with 4% paraformaldehyde (PFA) and labelled with TUNEL (Roche, cat# 11684795910). Cells were then immunostained (1:2000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... maxi- and minicircles were PCR amplified and labelled using PCR DIG Probe Synthesis Kit (Roche). The detection was done using DIG Luminiscent Detection Kit (Roche ...
-
bioRxiv - Microbiology 2021Quote: ... Bound primary antibodies were detected using biotin-conjugated anti-rat antibody (Roche, 1:1,000) and AlexaFluor594-conjugated streptavidin (Life Technologies ...
-
bioRxiv - Plant Biology 2020Quote: ... Digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen) ...
-
bioRxiv - Cancer Biology 2023Quote: ... OptiView DAB detection kit (proprietary, indirect, biotin free system, Roche Tissue Diagnostics, Indianapolis, IN) was used for primary antibody detection ...
-
bioRxiv - Developmental Biology 2023Quote: Anti-sense probes were synthesized using the DIG and Biotin-RNA labelling kit (Roche, 11277073910 and 11685597910 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Biotinylated RNAs were transcribed in vitro with Biotin-RNA Labeling Mix (Roche, Indianapolis, IN) and purified with quick spin RNA columns (Roche ...
-
bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ...
-
bioRxiv - Biochemistry 2020Quote: ... transfected with recombinant EMBacY BACs using X-tremeGENE DNA Transfection Reagent (Roche), and incubated for 72 h at 28 °C ...
-
bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse I (Dnase I recombinant RNAse-free, Roche Diagnostics). Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit ...
-
bioRxiv - Cell Biology 2020Quote: ... Genomic DNA was removed by digestion using DNase I Recombinant (Roche, 04716728001) and RiboLock RNase Inhibitor (Thermo Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... Recombinant baculovirus was generated by initial lipofection with Xtreme gene reagent (Roche) of Sf21 insect cells (Invitrogen) ...
-
bioRxiv - Neuroscience 2022Quote: ... and basic fibroblast growth factor (bFGF, 10 ng/ml; recombinant bovine, Roche). The cells were then plated in a 96-well plate in complete neurosphere medium containing DMEM/F-12 EGF and bFGF ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1x DNase buffer (500ul) and 200U recombinant DNase I (20ul, Roche, 04716728001) at 37 C in a shaker set at 100 RPM ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA contamination was removed through treatment with recombinant DNaseI (Roche Diagnostics, #04716728001) for 15 minutes at RT and column purification using Qiagen RNeasy Mini kit (#74106) ...
-
bioRxiv - Neuroscience 2023Quote: ... DNA contaminations were eliminated with DNase I recombinant (#04716728001; Roche Applied Science) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
-
bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
-
bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
-
bioRxiv - Microbiology 2020Quote: ... and then labelled using the DIG High Prime DNA Labelling and Detection Starter Kit I (Roche).
-
bioRxiv - Neuroscience 2021Quote: ... a fluorescein-labelled probe for GnRH3 mRNA was prepared using fluorescein RNA labelling mix (Roche Diagnostics), and it was used in combination with the DIG-labelled RNA probes for npff or gpr74-1 mRNA ...
-
bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin-labelled antisense RNA probes were produced using a DIG RNA labeling kit (Roche, #11277073910) according to the manufacturer’s protocol ...
-
Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... restriction endonucleases and RNA polymerases used for the generation of digoxigenin-labelled probes (Roche Applied Science) are provided in Table S2 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... labelled riboprobes were detected using anti-digoxigenin or anti-fluorescein antibodies conjugated to horseradish peroxidase (Roche). Fluorescent tagging was performed using Alexa Fluor 594 and 488 dyes with streptavidin Tyramide Signal Amplification (TSA ...
-
bioRxiv - Microbiology 2021Quote: ... Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche, 1603558) mix ...
-
bioRxiv - Plant Biology 2019Quote: ... using gene-specific digoxigenin-nucleotide-labelled DNA probes (Digoxigenin-11-dUTP alkali-labile, Roche, Basel, Switzerland) obtained using the same primer pairs and conditions as for the PCR ...
-
bioRxiv - Developmental Biology 2021Quote: ... and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
-
bioRxiv - Cell Biology 2023Quote: ... Dioxygenin (DIG)-labelled RNA probes were synthesized with T3 and T7 polymerase with reagents from Roche. Embryos were dechorionated with forceps and fixed in 4% paraformaldehyde in 1X PBS overnight ...
-
bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin-labelled probes were then detected by incubating with sheep Anti-Digoxigenin-POD Fab fragments (Roche) at a 1:50 dilution ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PlesSat01-48 and PlesSat02-200-B were labelled with digoxigenin-11-dUTP (both Roche, Mannheim, Germany).
-
bioRxiv - Developmental Biology 2023Quote: ... FITC-labelled probes were detected first by incubating with sheep Anti-Fluorescein-POD Fab fragments (Roche) at a 1:50 dilution ...
-
bioRxiv - Biophysics 2021Quote: ... at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche, Basel, Switzerland) coated coverglass surface at the other end through digoxigenin-antibody interactions ...
-
bioRxiv - Genomics 2020Quote: ... Probe DNA was nick-translated with either Digoxigenin-11-UTP or Biotin-16-UTP (Roche) using DNase I (Roche ...
-
bioRxiv - Plant Biology 2022Quote: ... The 5’-end biotin probes were generated using a DIG Gel Shift Kit (Roche, China) (Supplemental Table S8) ...
-
bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
-
bioRxiv - Plant Biology 2020Quote: ... 4 µg recombinant NAA50 was incubated with 100 µM Acetyl-Coenzyme A (Roche) in a 2X acetylation buffer (50mM Tris HCl pH 7.5 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The extracted RNA was then treated with DNase I recombinant RNase free (Roche) and the reverse transcription was done using the Super-Script IV (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 μg of total RNA was incubated with recombinant DNase I (Roche, 04716728001) for 30 min at 37°C and the reaction was stopped by incubation at 75°C for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... Residual genomic DNA contamination was removed by treatment with recombinant DNAse I (Roche). cDNA was generated using Superscript II reverse transcriptase and Oligo d(T ...
-
bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
-
bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
-
bioRxiv - Microbiology 2023Quote: DNase treatment was performed using recombinant RNase-free DNase I (Roche, Basel, Switzerland) according to the manufacturer’s protocols for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
-
bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... PAC-derived probes were extracted from bacterial cultures and fluorescently labelled by nick translation (Roche, Mannheim, Germany). Following hybridisation and washes according to published protocols (Federico et al. ...