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Citations for Roche :
351 - 400 of 1071 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... The incorporated digoxigenin-labelled nucleotides were detected by anti-digoxigenin-POD conjugated antibodies (Dilution 1.5 IU/ml; Roche, Cat no. 11633716001). Fluorescent signals were obtained by peroxidase-mediated Alexa Fluor 488 tyramide labelling (Alexa fluor 488 Tyramide SuperBoost kit ...
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bioRxiv - Developmental Biology 2024Quote: Pitx1 WISH was performed on E12.5 embryos with a digoxigenin-labelled Pitx1 antisense probe designed from a cloned antisense probe (PCR DIG Probe Synthesis Kit, Roche 11636090910). Experimental procedure followed the protocol outlined in (Kragesteen et al. ...
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Synthetic Biology 2021Quote: ... the efficiency of biotinylation was checked in a Western blot revealed with POD-anti-biotin (horseradish peroxidase conjugated with Streptavidin, Roche).
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bioRxiv - Biophysics 2019Quote: ... The central fragment of the molecule is flanked by oligonucleotides labelled either with digoxigenin (3’ end) or biotin (5’ end) that specifically bind either to a glass surface covered with Anti-digoxigenin (Roche) or to superparamagnetic beads (MyOne ...
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bioRxiv - Genetics 2021Quote: ... TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) assay was performed on retinal sections following the manuals (Cat # 11684795910, Roche).
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bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein G beads (Roche) were added for 2 h ...
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bioRxiv - Genetics 2020Quote: ... Protein A beads (Roche) were added for 2h ...
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bioRxiv - Immunology 2020Quote: ... Protein A agarose (Roche) beads were equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.01 mg/mL human insulin (Roche, Indianapolis, IN, USA), 100 IU penicillin (Mediatech) ...
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bioRxiv - Cancer Biology 2023Quote: ... human PR rabbit monoclonal antibody (Roche Diagnostics, 790-4296), and human Ki67 rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... human interleukin-2 (IL-2) (10 IU/ml, Roche), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Genomics 2020Quote: ... Hybridization of the digoxegenin (DIG)-labelled 31bp oligonucleotide probe and detection was carried out following the DIG High-Prime Labelling and Detection Starter Kit (Roche SKU 11745832910).
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bioRxiv - Neuroscience 2019Quote: ... 30μm thaw-mounted sections were hybridized overnight at 62°C with a DIG-labelled riboprobe for TVAG or HM3 (approximately 1:500; Roche, Cat. 11277073910) and then incubated in Blocking solution (600µl MABT ...
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bioRxiv - Developmental Biology 2022Quote: Single color staining of embryos hybridized with DIG-labelled riboprobes was performed using anti-DIG antibody conjugated to alkaline phosphatase (Roche, 1:6000) and BM purple (Roche ...
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bioRxiv - Microbiology 2022Quote: ... by the capillary method and hybridised with digoxigenin-labelled blaOXA- 58 and blaNDM-1-specific probes with an NBT/BCIP colour detection kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufactureŕs instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, IN, USA) and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0-1000 ng ...
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bioRxiv - Physiology 2024Quote: ... RNA probes were synthesised and labelled using the T7-ScribeTM Standard RNA IVT Kit (Madison, Wisconsin, United States) and Digoxigenin-11-UTP (Roche, Basel, Switzerland). Embryos at various stages were fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: Total proteins were extracted using protein extraction reagents (Roche Diagnostics Ltd., Shanghai, China) from the myocardial tissue of rats ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labeled LPA1-HA was detected using streptavidin beads (Pierce) and conjugated anti-HA antibody (3F10 from Roche Diagnostics; 1:1000).
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: Antisense and sense digoxigenin- and biotin-labeled riboprobes of AcerOr11 were synthesized using linearized pGEMHE plasmids containing appropriate insertion sequences as a template using the DIG and Biotin RNA Labeling Mix (Roche, Germany) and T7 RNA Polymerase (Roche ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Plant Biology 2023Quote: ... Southern blotting was performed with the digoxigenin (DIG)-labelled PCR-amplified gene fragment of HPT probe as described in the DIG System Manual (Roche, Inc., Basel, Switzerland). Genomic DNA (20 μg ...
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bioRxiv - Pathology 2024Quote: ... The resulting PCR products were then used as a template for generation of sense and antisense DIG-labelled probes using a DIG-nucleotide labelling kit (Roche, Indianapolis, IN, USA). Hybridised probes within the nematode tissues were detected using an anti-DIG antibody conjugated to alkaline phosphatase and its substrate ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting PCR products were then used as a template for generation of sense and antisense DIG-labelled probes using a DIG-nucleotide labelling kit (Roche, Indianapolis, IN, USA). Hybridised probes within the nematode tissues were detected using an anti-DIG antibody conjugated to alkaline phosphatase and its substrate ...
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bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
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bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
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bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
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bioRxiv - Immunology 2019Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The A20 cells were grown in RPMI-1640 (Gibco ...