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Citations for Roche :
1 - 50 of 1071 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... with biotin-labelled UTP (Roche). The RNA was then purified using the MEGAclear kit (Ambion ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Probes were labelled with biotin using Biotin-High Prime (Roche). Hybridization and detection of biotinylated probes was performed using the North2South™ Chemiluminescent Hybridization and Detection Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... and labelled with Biotin using Biotin RNA Labelling Mix (Roche, #11685597910) according to the manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The 18S rDNA probe labelled by biotin (Roche) has been prepared from genomic DNA of the slow worm Anguis fragilis ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin or biotin-labelled probes were synthesized using the labelling kit (Roche). The template DNA for the Osi10b RNA antisense probe was amplified with the primer set (Forward ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Genomics 2022Quote: ... Clone probes were PCR labelled with biotin-16-dUTP (from Roche Applied Science). Hybridization was performed over-night ...
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bioRxiv - Genomics 2021Quote: Probes 1-3 were labelled with digoxigenin-11-dUTP or biotin-11-dUTP (Roche) using BioPrime® Array CGH ...
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bioRxiv - Immunology 2021Quote: ... 10 ng/ml recombinant human EGF (Roche, Ireland), 1 μg/ml hydrocortisone (Sigma ...
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bioRxiv - Genomics 2020Quote: ... were labelled with biotin 16-dUTP using a nick translation kit (Roche Diagnostics, Basel, Switzerland) and hybridized to metaphase chromosomes overnight at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... The 18S rDNA probe was labelled either with biotin-16-dUTP (Roche Diagnostics, Basel, Switzerland) or DNP-11-dUTP (Jena Bioscience ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... and human recombinant IL-2 (30 U/ml, Roche) for 72 h.
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bioRxiv - Immunology 2023Quote: ... and 10 ng/ml recombinant human IL-2 (Roche). For activation of murine CD4+ T cells ...
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bioRxiv - Plant Biology 2019Quote: The satellite-specific probes were labelled by PCR in the presence of biotin-16-dUTP (Roche). We used nick translation to mark the probes for the ribosomal genes ...
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bioRxiv - Molecular Biology 2021Quote: ... BACPAC Resources) were labelled with digoxigenin-dUTP or biotin-dUTP using a nick translation kit (Roche), denatured (95°C ...
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bioRxiv - Immunology 2023Quote: ... 100 IU/ml of recombinant human IL-2 (Roche Diagnostics) or 10 μg/ml of SIVmac251 Env gp130 (ImmunoDx) ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA probes were labelled by nick translation with either biotin-16-dUTP or digoxigenin-11-dUTP (Roche). For simultaneous IF and RNA FISH ...
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bioRxiv - Molecular Biology 2020Quote: ... Probes were labelled using a nick translation reaction with the uridine analogues biotin-16-dUTP (Roche, CatNo 11093070910) or digoxigenin-11-dUTP (Roche ...
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bioRxiv - Genomics 2022Quote: ... Cycle Pure Kit (Omega) and then labelled with either biotin–16-dUTP or digoxigenin–11-dUTP (Roche Diagnostics) using the BioPrime Array CGH random priming kit (Invitrogen) ...
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bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...
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bioRxiv - Molecular Biology 2023Quote: For in vitro RNA transcription of LINC00941 the Sp6 RNA Polymerase was used according to the manufacturer’s instructions with the exception that LINC00941 was labelled with biotin-16-UTP (Roche). 2 µg of biotin-16-labelled LINC00941 were incubated with protein lysate of 3*107 keratinocytes ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative Western blot analysis was employed to determine concentration of recombinant protein using a recombinant GFP standard (Roche, 11814524001) with a defined concentration of 1 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Molecular Biology 2021Quote: ... and biotin (Biotin RNA Labeling Mix 10 × conc, Roche) were in vitro transcribed using in vitro Transcription T7 Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... or biotin (Biotin RNA Labeling Mix 10 × conc, Roche) were transcribed using in vitro Transcription T7 Kit (Takara) ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Genetics 2022Quote: ... MYC-tagged or FLAG-tagged recombinant proteins were captured using ~5μg antibody and Protein-Agarose beads (Roche Applied Science, 11243233001) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... DIG-labelled (Roche 11277073910) RNA probes were transcribed via the T3 promotor from 1ug of purified DNA template and cleaned via ethanol-salt purification with GenElute linear polyacrylamide (Sigma 56575-1ML ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labelled probes were detected with FITC-labelled antibodies (primary antibody from Roche, secondary antibodies from Jackson ImmunoResearch) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to PVDF membranes and labelled overnight with anti-HA (0.01 μg/mL, Roche clone 3F10), anti-VE-Cadherin (0.5 μg.ml−1 ...
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bioRxiv - Genomics 2020Quote: ... biotin-16-dUTP (Roche), and/or DNP-11-dUTP (PerkinElmer) ...
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bioRxiv - Biophysics 2022Quote: ... biotin-16-dUTP (Roche), biotin-14-dCTP (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Plant Biology 2021Quote: ... labelled with dig-UTP (Roche) was synthesized for the full-length cDNA for CyperusNTT using the Invitrogen SuperscriptIII kit according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... which are UPL labelled (Roche) and gene-specific primers were mixed with TaqMan Fast Universal PCR Master Mix (Appliued Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxigenin-labelled (DIG; Roche 11277073910) and fluorescein-labelled (Fluo ...
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bioRxiv - Neuroscience 2023Quote: ... using DIG labelled nucleotides (Roche). Following DNA digestion and RNA purification ...
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bioRxiv - Developmental Biology 2023Quote: ... and digoxigenin-labelled dUTPs (Roche). Alternatively ...
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bioRxiv - Bioengineering 2021Quote: ... pastoris culture medium containing the recombinant protein was purified by affinity chromatography Ni-NTA agarose (Roche).
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bioRxiv - Neuroscience 2023Quote: ... and NexCreERT2::R26R-tdT male mice received intraperitoneal injections (i.p.) of recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche) or PL (solvent solution ...