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Citations for Roche :
1851 - 1900 of 6956 citations for 6 IODO 2 PIPERAZIN 1 YL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized at 37°C for 2–4 hrs in digoxigenin-synthesis reaction mixture with T7 RNA polymerase (Roche). After synthesis ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended and homogenized in buffer B supplemented with 2 mM MgCl2 and 1x protease inhibitor (Roche). The protein was extracted by incubating with 1% GDN (Anatrace ...
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bioRxiv - Molecular Biology 2023Quote: ... uteri were harvested from pregnant mice at 4.5 days post coitus and washed with cold swelling buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 1X Protease Inhibitor Cocktail (PIC, Roche, 11836170001)) immediately after collection ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Neuroscience 2023Quote: Hippocampi were homogenized in 500µl of RIPA medium lysis buffer (Beyotime, #P0013E-2) in the presence of protease inhibitor cocktail (Roche, #11836170001). Samples were centrifuged at 10,000 × g for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 mL dissociation buffer (RPMI-1640 (Cytiva) with 25 mM HEPES and 10 µg/mL gentamicin sulfate) supplemented with 2 mg/mL Collagenase-D (Roche) and 100 U DNaseI (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Immunology 2024Quote: ... digested three times (DMEM high glucose with 2% FBS, 100 µg/ml DNase I, and 100 µg/ml Liberase TM, Roche) or until no tissue fragments remained ...
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bioRxiv - Neuroscience 2024Quote: ... for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Table S2) and measured with a LightCyclerTM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels.
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bioRxiv - Cell Biology 2024Quote: ... The cells were pelleted by centrifugation (750 x g, 2 min, 4 °C) and resuspended in 50 μl vPBS containing EDTA-free protease inhibitor (Roche). The cells were fixed by addition of 0.5 ml ice-cold fixation solution (4% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% Cholesteryl Hemissucinate (CHS) (Avanti Pola lipids) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl and 0.5% SMALP200 (CubeBiotech GmbH) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were lysed in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 01% SDS, 1 mM EDTA, supplemented with protease inhibitor cocktail [Complete Roche Mini, 1 tablet per 50 ml]) and spun at 20.000 g to eliminate cell debris ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT) supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 1 mM EDTA) containing EDTA-free protease inhibitor cocktail (mini-complete, Roche), 20 μM MG132 and 25 μg/ml ALLN followed by boiling in SDS sample buffer ...
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bioRxiv - Microbiology 2019Quote: ... 1% Sodium Deoxycholate and 1% Triton X-100) supplemented with Complete Protease Inhibitors without EDTA (Roche). Cellular debris were removed by centrifugation at 17,000g ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM NA3VO4 and 1 μg/ml leupeptin supplemented with a complete mini protease inhibitor (Roche). Proteins (30–40 μg ...
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bioRxiv - Genetics 2019Quote: ... rat polyclonal anti-Lid (1:500) [13] and mouse monoclonal anti-GFP (Roche #118144600001; 1:200). Secondary antibodies were goat anti-rabbit antibodies (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Protein translation was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, 11411446001, Roche Diagnostics) and cultures were incubated overnight at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg ml−1 lysozyme and a tablet of complete EDTA-free Protease Inhibitor Cocktail (Roche). Cells were lysed by sonication on ice for 3 min and centrifuged at 48,300 × g for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 mM dithiothreitol) containing 1% Triton X-100 and protease and phosphatase inhibitor cocktails (Roche) (HS-TX buffer ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000, Roche 11093274910) in Blocking Buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 1% deoxycholic acid and 1% Nonidet P-40 [NP-40]) containing protease inhibitor cocktail (Roche Diagnostics) and placed on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mg/ml bromophenol blue) and 1 μl proteinase K (14–22 mg/ml, Roche). The reaction was further incubated for 15 mins at 30°C for deproteination ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% NP40 substitute) with complete protease inhibitor cocktail (Roche Cat. #11836145001; 1 tablet per 50 mL). Samples were homogenized twice for 30 seconds at 6.5m/s in a fast-prep machine with an incubation at 4 °C for 5 min between runs ...
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bioRxiv - Genetics 2022Quote: ... 10 ul of Protease Inhibitor Cocktail (Roche; 1 tablet diluted in 1 ml of DEPC H2O), and 0.5 ul of Ribolock) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then blocked in TNT/block [0.5% blocking reagent (PerkinElmer FP1012)/TNT] for 1 hour at RT before ON incubation with anti-DIG (1:100, Roche 11207733910) or anti-Fluo (1:250 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP) supplemented with Protease Inhibitor Cocktail (1 tablet/30 ml; complete, EDTA-free, Roche) and sonicated on ice ...
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bioRxiv - Microbiology 2023Quote: ... 1% Triton X-100 & 1% Deoxycholate) supplemented with a protease mixture inhibitor (Roche Complete, EDTA free). Lysates were either immunoprecipitated using GFP-trap agarose beads (ChromoTek ref# gta-10 ...
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bioRxiv - Neuroscience 2023Quote: ... resuspension in 1 volume of TBS containing 1 mM PMSF and cOmplete protease inhibitor cocktail (Roche), and dropwise addition to liquid nitrogen to snap freeze ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium deoxycholate) supplemented with 1 mM PMSF and 1× protease inhibitor cocktail (Roche; Cat# 04693132001). The cells were broken using Zirconia/Silica beads (BioSpec ...
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bioRxiv - Microbiology 2023Quote: ... at a 1:1 ratio in a 96-well deep-well extraction plate (Roche Diagnostics GmbH), covered with a MagNA Pure Sealing Foil (06241603001 ...
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bioRxiv - Microbiology 2023Quote: ... 1% sodium deoxycholate and 1% Triton X-100) supplemented with Complete protease inhibitors without EDTA (Roche). Cellular debris was removed by centrifugation at 17,000 × g ...
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bioRxiv - Neuroscience 2023Quote: ... DRG were incubated for 1 hour at 37 °C with 1 mg/ml dispase II (Roche) and 2 mg/ml collagenase A (Roche ...
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bioRxiv - Genomics 2024Quote: ... Miniaturization involved testing libraries at 1/6th (IDT mini) or 1/8th (Roche mini, Illumina mini) of reaction volume tailoring input DNA to each kit between 24-45 ng total (Table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 s) in 1 ml lysis buffer [buffer A including 1 mM PMSF (phenylmethylsulfonyl fluoride, Roche), 20 U DNase I (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with DAPI (1/2000 in sterile PBS from 1 mg/ml stock, Roche). Sorted cells were re-suspended in medium to reach a 2.5 million cells/ml concentration ...
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bioRxiv - Biochemistry 2024Quote: ... 35 mM imidazole) + protease inhibitors (1 tablet / 50 ml Roche Complete EDTA-free, 1 mM AEBSF), lysozyme ...
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bioRxiv - Biophysics 2019Quote: ... 1 tablet of protease inhibitors (Roche) per 100 mL buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% BSA Fraction V (Roche) at pH 7.4] and seeded at a density of 50–75 × 103 cells/plate on laminin (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml Pefabloc SC (Roche), and EDTA-free protease inhibitor cocktail (cOmplete Tablets ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml Pefabloc SC (Roche)) using an EmulsiFlex C3 homogenizer (Avestin) ...